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26.05.2023 | Research

Loss of Caveolin-1 Expression in Tumor Cells is Associated with Increased Aggressiveness and Cell Invasion in Oral Squamous Cell Carcinoma

verfasst von: Rebeca Barros Nascimento, Katiúcia Batista Silva Paiva, Maija Risteli, Luiz Henrique Santos Silva, Camila Oliveira Rodini, Maria Fernanda Setúbal Destro Rodrigues, Rafael De Cicco, Rossana Verónica Mendoza Lopez, Tuula Anneli Salo, Fábio Daumas Nunes, Flávia Caló Aquino Xavier

Erschienen in: Head and Neck Pathology | Ausgabe 3/2023

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Abstract

Background

Changes in Caveolin-1 (CAV-1) expression are related to tumorigenesis. The aim of this study was to evaluate the role of CAV-1 in tumor progression in oral squamous cell carcinoma (SCC) tissue samples and the effect of CAV-1 silencing on two oral tongue SCC (OTSCC) cell lines (SCC-25, from a primary tumor, and HSC-3 from lymph node metastases).

Methods

Mycroarray hybridization, mRNA expression, and immunohistochemistry were performed on OSCC tissue samples and corresponding non-tumoral margin tissues. The effects of CAV-1 silencing (siCAV-1) on cell viability, membrane fluidity, on the expression of epithelial to mesenchymal transition (EMT) markers and on cell migration and invasion capacity of OTSCC cell lines were evaluated.

Results

Microarray showed a greater CAV-1 expression (1.77-fold) in OSCC tumors than in non-tumoral tissues and 2.0-fold more in less aggressive OSCCs. However, significant differences in CAV-1 gene expression were not seen between tumors and non-tumoral margins nor CAV-1 with any clinicopathological parameters. CAV-1 protein was localized both in carcinoma and in spindle cells of the tumor microenvironment (TME), and CAV-1 positive TME cells were associated with smaller/more aggressive tumors, independent of the carcinoma cells’ expression. Silencing of CAV-1 increased cell viability only in SCC-25 cells. It also stimulated the invasion of HSC-3 cells and increased ECAD and BCAT mRNA in these cells; however, the protein levels of the EMT markers were not affected.

Conclusion

Decreased expression of CAV-1 by tumor cells in OSCC and an increase in the TME were associated with increased cell invasiveness and tumor aggressiveness.

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Titel: Loss of Caveolin-1 Expression in Tumor Cells is Associated with Increased Aggressiveness and Cell Invasion in Oral Squamous Cell Carcinoma
verfasst von: Rebeca Barros Nascimento
Katiúcia Batista Silva Paiva
Maija Risteli
Luiz Henrique Santos Silva
Camila Oliveira Rodini
Maria Fernanda Setúbal Destro Rodrigues
Rafael De Cicco
Rossana Verónica Mendoza Lopez
Tuula Anneli Salo
Fábio Daumas Nunes
Flávia Caló Aquino Xavier
Publikationsdatum: 26.05.2023
Verlag: Springer US
Erschienen in: Head and Neck Pathology / Ausgabe 3/2023
Elektronische ISSN: 1936-0568
DOI: https://doi.org/10.1007/s12105-023-01562-w

Neu im Fachgebiet Pathologie

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Loss of Caveolin-1 Expression in Tumor Cells is Associated with Increased Aggressiveness and Cell Invasion in Oral Squamous Cell Carcinoma

Abstract

Background

Methods

Results

Conclusion

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Abstract

Background

Methods

Results

Conclusion

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