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Erschienen in: Lasers in Medical Science 4/2021

22.08.2020 | Original Article

Lycium barbarum polysaccharide fraction associated with photobiomodulation protects from epithelium thickness and collagen fragmentation in a model of cutaneous photodamage

verfasst von: Lia Mara Grosso Neves, Carla Roberta Tim, Elaine Medeiros Floriano, Lucimar Retto da Silva de Avó, João Batista Fernandes, Nivaldo Antonio Parizotto, Marcia Regina Cominetti

Erschienen in: Lasers in Medical Science | Ausgabe 4/2021

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Abstract

Ultraviolet radiation (UVR) is the major etiologic agent of cutaneous photoaging, and different strategies are used to prevent and treat this condition. The polysaccharide fraction (LBPF) isolated from Lycium Barbarum fruits (goji berry) contains several active ingredients with antioxidant, immune system modulation, and antitumor effects. In addition, the photobiomodulation (PBM) is widely applied in photoaging treatment. This study investigated the effects of LBPF and PBM against the UVR-induced photodamage in the skin of hairless mice. The mice were photoaged for 6 weeks in a chronic and cumulative exposure regimen using a 300-W incandescent lamp that simulates the UVR effects. From the third to the sixth week of photoaging induction, the animals received topical applications of LBPF and PBM, singly or combined, in different orders (first LBPF and then PBM and inversely), three times per week after each session of photoaging. After completion of experiments, the dorsal region skin was collected for the analysis of thickness, collagen content, and metalloproteinases (MMP) levels. A photoprotective potential against the increase of the epithelium thickness and the fragmentation of the collagen fibers was achieved in the skin of mice treated with LBPF or PBM singly, as well as their combination. All treatments maintained the skin collagen composition, except when PBM was applied after the LBPF. However, no treatment protected against the UVR-induced MMP increase. Taken together, we have shown that the LBPF and PBM promote a photoprotective effect in hairless mice skin against epidermal thickening and low collagen density. Both strategies, singly and combined, can be used to reduce the UVR-induced cutaneous photoaging.
Literatur
4.
Zurück zum Zitat Armstrong BK, Kricker A (2001) The epidemiology of UV induced skin cancer. J Photochem Photobiol B 63(1-3):8–18CrossRef Armstrong BK, Kricker A (2001) The epidemiology of UV induced skin cancer. J Photochem Photobiol B 63(1-3):8–18CrossRef
10.
Zurück zum Zitat Zhang Q, Lv X, Wu T, Ma Q, Teng A, Zhang Y, Zhang M (2015) Composition of Lycium barbarum polysaccharides and their apoptosis-inducing effect on human hepatoma SMMC-7721 cells. Food Nutr Res 59:28696CrossRef Zhang Q, Lv X, Wu T, Ma Q, Teng A, Zhang Y, Zhang M (2015) Composition of Lycium barbarum polysaccharides and their apoptosis-inducing effect on human hepatoma SMMC-7721 cells. Food Nutr Res 59:28696CrossRef
12.
Zurück zum Zitat Migliato KF, Chiosini MA, Mendonca FA, Esquisatto MA, Salgado HR, Santos GM (2011) Effect of glycolic extract of Dillenia indica L. combined with microcurrent stimulation on experimental lesions in Wistar rats. Wounds 23(5):111–120PubMed Migliato KF, Chiosini MA, Mendonca FA, Esquisatto MA, Salgado HR, Santos GM (2011) Effect of glycolic extract of Dillenia indica L. combined with microcurrent stimulation on experimental lesions in Wistar rats. Wounds 23(5):111–120PubMed
16.
Zurück zum Zitat Avci P, Gupta A, Sadasivam M, Vecchio D, Pam Z, Pam N, Hamblin MR (2013) Low-level laser (light) therapy (LLLT) in skin: stimulating, healing, restoring. Semin Cutan Med Surg 32(1):41–52PubMedPubMedCentral Avci P, Gupta A, Sadasivam M, Vecchio D, Pam Z, Pam N, Hamblin MR (2013) Low-level laser (light) therapy (LLLT) in skin: stimulating, healing, restoring. Semin Cutan Med Surg 32(1):41–52PubMedPubMedCentral
20.
Zurück zum Zitat Cheng J, Zhou ZW, Sheng HP, He LJ, Fan XW, He ZX, Sun T, Zhang X, Zhao RJ, Gu L, Cao C, Zhou SF (2015) An evidence-based update on the pharmacological activities and possible molecular targets of Lycium barbarum polysaccharides. Drug Des Dev Ther 9:33–78. https://doi.org/10.2147/dddt.s72892CrossRef Cheng J, Zhou ZW, Sheng HP, He LJ, Fan XW, He ZX, Sun T, Zhang X, Zhao RJ, Gu L, Cao C, Zhou SF (2015) An evidence-based update on the pharmacological activities and possible molecular targets of Lycium barbarum polysaccharides. Drug Des Dev Ther 9:33–78. https://​doi.​org/​10.​2147/​dddt.​s72892CrossRef
21.
Zurück zum Zitat Fisher GJ (2005) The pathophysiology of photoaging of the skin. Cutis 75(2 Suppl):5–8 discussion 8-9PubMed Fisher GJ (2005) The pathophysiology of photoaging of the skin. Cutis 75(2 Suppl):5–8 discussion 8-9PubMed
Metadaten
Titel
Lycium barbarum polysaccharide fraction associated with photobiomodulation protects from epithelium thickness and collagen fragmentation in a model of cutaneous photodamage
verfasst von
Lia Mara Grosso Neves
Carla Roberta Tim
Elaine Medeiros Floriano
Lucimar Retto da Silva de Avó
João Batista Fernandes
Nivaldo Antonio Parizotto
Marcia Regina Cominetti
Publikationsdatum
22.08.2020
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 4/2021
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-020-03132-w

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