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30.04.2021 | Original Article

Microbicidal effect of 405-nm blue LED light on Candida albicans and Streptococcus mutans dual-species biofilms on denture base resin

verfasst von: Chiaki Tsutsumi-Arai, Yuki Arai, Chika Terada-Ito, Takahiro Imamura, Seiko Tatehara, Shinji Ide, Noriyuki Wakabayashi, Kazuhito Satomura

Erschienen in: Lasers in Medical Science | Ausgabe 2/2022

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Abstract

This study investigated: (1) the microbicidal effect of 405-nm blue LED light irradiation on biofilm formed by Candida albicans hyphae and Streptococcus mutans under dual-species condition on denture base resin, (2) the generation of intracellular reactive oxygen species (ROS) induced by irradiation, and (3) the existence of intracellular porphyrins, which act as a photosensitizer. Denture base resin specimens were prepared and C. albicans and S. mutans dual-species biofilms were allowed to form on the specimens. The biofilms were irradiated with 405-nm blue LED light and analyzed using the colony-forming unit assay, fluorescence microscopy, and scanning electron microscopy (SEM). Single-species biofilms of C. albicans and S. mutans formed on the specimens were irradiated with 405-nm blue LED light. After the irradiation, the intracellular ROS levels in C. albicans and S. mutans cells were measured. In addition, the level of intracellular porphyrins in C. albicans and S. mutans were measured. Irradiation for more than 30 min significantly inhibited the colony formation ability of C. albicans and S. mutans. Fluorescence microscopy revealed that almost all C. albicans and S. mutans cells were killed by irradiation. SEM images showed various cell damage patterns. Irradiation led to the generation of intracellular ROS and porphyrins were present in both C. albicans and S. mutans cells. In conclusion, irradiation with 405-nm blue light-emitting diode light for 40 min effectively disinfect C. albicans hyphae and S. mutans dual-species biofilms and possibly react with intracellular porphyrins resulting in generation of ROS in each microorganism.

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Titel: Microbicidal effect of 405-nm blue LED light on Candida albicans and Streptococcus mutans dual-species biofilms on denture base resin
verfasst von: Chiaki Tsutsumi-Arai
Yuki Arai
Chika Terada-Ito
Takahiro Imamura
Seiko Tatehara
Shinji Ide
Noriyuki Wakabayashi
Kazuhito Satomura
Publikationsdatum: 30.04.2021
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 2/2022
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-021-03323-z

Springer Medizin

Abstract

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