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Erschienen in: Lasers in Medical Science 3/2022

17.11.2021 | Original Article

Photobiomodulation therapy upregulates the growth kinetics and multilineage differentiation potential of human dental pulp stem cells—an in vitro Study

verfasst von: Lama E. Dawoud, Enas M. Hegazy, Rania A. Galhom, Mervat M. Youssef

Erschienen in: Lasers in Medical Science | Ausgabe 3/2022

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Abstract

This study aims to evaluate the impact of red LED irradiation on the viability, proliferation, colonogenic potential, markers expression along with osteogenic and chondrogenic differentiation of dental pulp stem cells. DPSCs were isolated from sound human permanent teeth using enzymatic digestion method and seeded with regular culture media. Cells at P4 were irradiated using red LED Light (627 nm, 2 J/cm2) and examined for growth kinetics, and multilineage differentiation using the appropriate differentiation media. The irradiated groups showed an increase in cellular growth rates, cell viability, clonogenic potential, and decrease in population doubling time compared to the control group. Cells of the irradiated groups showed enhanced differentiation towards osteogenic and chondrogenic lineages as revealed by histochemical staining using alizarin red and alcian blue stains. Photobiomodulation is an emerging promising element of tissue engineering triad besides stem cells, scaffolds, and growth factors.

Graphical abstract

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Metadaten
Titel
Photobiomodulation therapy upregulates the growth kinetics and multilineage differentiation potential of human dental pulp stem cells—an in vitro Study
verfasst von
Lama E. Dawoud
Enas M. Hegazy
Rania A. Galhom
Mervat M. Youssef
Publikationsdatum
17.11.2021
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 3/2022
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-021-03461-4

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