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Erschienen in: Cardiovascular Toxicology 9/2022

09.07.2022

Salvianolic Acid B Alleviates Myocardial Ischemia Injury by Suppressing NLRP3 Inflammasome Activation via SIRT1-AMPK-PGC-1α Signaling Pathway

verfasst von: Qingju Li, Zhi Zuo, Yunzheng Pan, Qi Zhang, Li Xu, Baoping Jiang

Erschienen in: Cardiovascular Toxicology | Ausgabe 9/2022

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Abstract

Salvianolic acid B (SalB) has been extensively investigated in our laboratory for myocardial ischemia (MI) disease. This study mainly aimed to illustrate the relationship between SIRT1 and the therapeutic effect of SalB on MI in rats and hypoxia damage in H9c2 cells. Furthermore, whether the antagonism of NLRP3 by SalB in the injuries mentioned above is related to SIRT1-AMPK-PGC-1α pathway-mediated mitochondrial biogenesis was further investigated. In vivo, 24 h after MI surgery, we found that SalB effectively reduced ST-segment elevation, myocardial infarct size enlargement, cardiac injury markers, myocardial structural abnormalities, and myocardial apoptotic cells in MI injury rats. In vitro, after 4 h of hypoxia exposure, SalB alleviated cell injury, inhibited the production of ROS and IL-1β, and prevented the loss of mitochondrial membrane potential (MMP). Besides, SalB downregulated the critical components of the NLRP3 inflammasome and upregulated the SIRT1-AMPK-PGC-1α signaling pathway-related molecules in myocardial tissues and H9c2 cells. However, all the above protective effects of SalB on MI could be offset by EX527. Taken together, our findings indicated that SalB could attenuate MI injury by targeting NLRP3, which is at least partially dependent on the SIRT1/AMPK/PGC-1α signaling pathway.
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Literatur
2.
Zurück zum Zitat Nanasato, M., Matsumoto, N., Nakajima, K., Chikamori, T., Moroi, M., Takehana, K., Momose, M., Nishina, H., Kasai, T., Yoda, S., Kiso, K., Yamamoto, H., Nishimura, S., Yamashina, A., Kusuoka, H., Hirayama, A., & Nishimura, T. (2018). Prognostic impact of reducing myocardial ischemia identified using ECG-gated myocardial perfusion SPECT in Japanese patients with coronary artery disease: J-ACCESS 4 study. International Journal of Cardiology, 267, 202–207. https://​doi.​org/​10.​1016/​j.​ijcard.​2018.​05.​090 CrossRefPubMed Nanasato, M., Matsumoto, N., Nakajima, K., Chikamori, T., Moroi, M., Takehana, K., Momose, M., Nishina, H., Kasai, T., Yoda, S., Kiso, K., Yamamoto, H., Nishimura, S., Yamashina, A., Kusuoka, H., Hirayama, A., & Nishimura, T. (2018). Prognostic impact of reducing myocardial ischemia identified using ECG-gated myocardial perfusion SPECT in Japanese patients with coronary artery disease: J-ACCESS 4 study. International Journal of Cardiology, 267, 202–207. https://​doi.​org/​10.​1016/​j.​ijcard.​2018.​05.​090 CrossRefPubMed
6.
Zurück zum Zitat Girerd, N., Cleland, J., Anker, S. D., Byra, W., Lam, C. S. P., Lapolice, D., Mehra, M. R., van Veldhuisen, D. J., Bresso, E., Lamiral, Z., Greenberg, B., & Zannad, F. (2022). Inflammation and remodeling pathways and risk of cardiovascular events in patients with ischemic heart failure and reduced ejection fraction. Scientific Reports, 12(1), 022–12385. https://​doi.​org/​10.​1038/​s41598-022-12385-0 CrossRef Girerd, N., Cleland, J., Anker, S. D., Byra, W., Lam, C. S. P., Lapolice, D., Mehra, M. R., van Veldhuisen, D. J., Bresso, E., Lamiral, Z., Greenberg, B., & Zannad, F. (2022). Inflammation and remodeling pathways and risk of cardiovascular events in patients with ischemic heart failure and reduced ejection fraction. Scientific Reports, 12(1), 022–12385. https://​doi.​org/​10.​1038/​s41598-022-12385-0 CrossRef
12.
18.
Zurück zum Zitat Chao, Y. M., Wu, K. L. H., Tsai, P. C., Tain, Y. L., Leu, S., Lee, W. C., & Chan, J. Y. H. (2020). Anomalous AMPK-regulated angiotensin AT(1)R expression and SIRT1-mediated mitochondrial biogenesis at RVLM in hypertension programming of offspring to maternal high fructose exposure. Journal of Biomedical Science, 27(1), 020–00660. https://​doi.​org/​10.​1186/​s12929-020-00660-z CrossRef Chao, Y. M., Wu, K. L. H., Tsai, P. C., Tain, Y. L., Leu, S., Lee, W. C., & Chan, J. Y. H. (2020). Anomalous AMPK-regulated angiotensin AT(1)R expression and SIRT1-mediated mitochondrial biogenesis at RVLM in hypertension programming of offspring to maternal high fructose exposure. Journal of Biomedical Science, 27(1), 020–00660. https://​doi.​org/​10.​1186/​s12929-020-00660-z CrossRef
27.
Zurück zum Zitat Liu, H., Liu, W., Qiu, H., Zou, D., Cai, H., Chen, Q., Zheng, C., & Xu, D. (2020). Salvianolic acid B protects against myocardial ischaemia-reperfusion injury in rats via inhibiting high mobility group box 1 protein expression through the PI3K/Akt signalling pathway. Naunyn-Schmiedeberg’s Archives of Pharmacology, 393(8), 1527–1539. https://​doi.​org/​10.​1007/​s00210-019-01755-7 CrossRefPubMed Liu, H., Liu, W., Qiu, H., Zou, D., Cai, H., Chen, Q., Zheng, C., & Xu, D. (2020). Salvianolic acid B protects against myocardial ischaemia-reperfusion injury in rats via inhibiting high mobility group box 1 protein expression through the PI3K/Akt signalling pathway. Naunyn-Schmiedeberg’s Archives of Pharmacology, 393(8), 1527–1539. https://​doi.​org/​10.​1007/​s00210-019-01755-7 CrossRefPubMed
42.
Zurück zum Zitat Yang, X. Y., He, K., Pan, C. S., Li, Q., Liu, Y. Y., Yan, L., Wei, X. H., Hu, B. H., Chang, X., Mao, X. W., Huang, D. D., Wang, L. J., Hu, S. W., Jiang, Y., Wang, G. C., Fan, J. Y., Fan, T. P., & Han, J. Y. (2015). 3, 4-dihydroxyl-phenyl lactic acid restores NADH dehydrogenase 1 α subunit 10 to ameliorate cardiac reperfusion injury. Science Reports. https://​doi.​org/​10.​1038/​srep10739 CrossRef Yang, X. Y., He, K., Pan, C. S., Li, Q., Liu, Y. Y., Yan, L., Wei, X. H., Hu, B. H., Chang, X., Mao, X. W., Huang, D. D., Wang, L. J., Hu, S. W., Jiang, Y., Wang, G. C., Fan, J. Y., Fan, T. P., & Han, J. Y. (2015). 3, 4-dihydroxyl-phenyl lactic acid restores NADH dehydrogenase 1 α subunit 10 to ameliorate cardiac reperfusion injury. Science Reports. https://​doi.​org/​10.​1038/​srep10739 CrossRef
Metadaten
Titel
Salvianolic Acid B Alleviates Myocardial Ischemia Injury by Suppressing NLRP3 Inflammasome Activation via SIRT1-AMPK-PGC-1α Signaling Pathway
verfasst von
Qingju Li
Zhi Zuo
Yunzheng Pan
Qi Zhang
Li Xu
Baoping Jiang
Publikationsdatum
09.07.2022
Verlag
Springer US
Erschienen in
Cardiovascular Toxicology / Ausgabe 9/2022
Print ISSN: 1530-7905
Elektronische ISSN: 1559-0259
DOI
https://doi.org/10.1007/s12012-022-09760-8

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