Supratentorial extra-axial RELA fusion-positive ependymoma misdiagnosed as meningioma by intraoperative histological and cytological examinations: a case report

Ependymoma is a glial tumor in the central nervous system and corresponds to World Health Organization (WHO) grade II category [1]. Anaplastic ependymoma is histologically a higher-grade tumor than classic ependymoma, corresponding to WHO grade III category, characterized by features such as a high nuclear-to-cytoplasmic (N/C) ratio, elevated mitotic counts, high cell density, widespread microvascular proliferation, and necrosis [2]. However, a definite association between histological grade and biological behavior/survival of ependymomas has not been established [1, 2].

Molecular classification of tumor has been proposed as a prognostic tool for ependymal tumors [3]. Poor prognosis is mainly associated with supratentorial classic/anaplastic ependymomas with RELA fusion gene [3]. RELA fusion-positive ependymomas have become a distinct entity in the classification of ependymal tumors since 2006 [4], and are now classified as grade II or III, depending on histopathological anaplastic features. Recent studies have shown that L1CAM immunohistochemistry can be an alternative for RELA fusion gene detection [5].

Ependymomas usually arise in or near the ventricular system [1]. Intracranial extra-axial ependymoma is a rare tumor, and to date, only 27 cases have been reported. Among them, 21 were confirmed as supratentorial extra-axial ependymomas (SEAEs), 9 of which were reported as SEAE attached to the dura mater (Table 1) [6‐13]. Dura-attached SEAEs are often preoperatively misdiagnosed as meningiomas on the basis of radiological findings (Table 1).

A 26-year-old Japanese male presented with severe headache and nausea for approximately 3 weeks. He had a prior episode of suspected convulsion of which magnetic resonance imaging (MRI) revealed a cystic nodular lesion measuring approximately 60 mm in diameter, located in the left parietal lobe of the cerebrum. The patient was administered medication, and symptoms were subsequently relieved. However, 12 days later, he experienced worsened symptoms and was transferred and admitted to the emergency department of the University of Miyazaki Hospital.

MRI showed a mass of 70 × 53 × 57 mm in the left temporoparietal lobe (Fig. 1a–c). A solid element with slight hypointensity on T1-weighted and hyperintensity on T2-weighted images along the dura mater was noted. Cystic changes were likely associated with the sanguineous fluid. A peritumoral band with hyperintensity on T2-weighted image was observed at the periphery of the lesion, suggesting an extra-axial lesion (Fig. 1b). A dural tail sign was also noted on the gadolinium-enhanced T1-weighted image (Fig. 1c). The lesion appeared to be focally infiltrative to the brain surface, but no apparent connection to the ventricle was observed (Fig. 1a–c). Preoperative clinical diagnosis was meningioma, but hemangiopericytoma and ependymoma were included as differential diagnoses. Left parietotemporal craniotomy was performed. The tumor was an extra-axial mass and had partially infiltrated the dura mater and surface of the brain parenchyma in the left temporal lobe. Small pieces of tumor tissue were removed for intraoperative diagnosis. Under an emergency setting, near-total resection was achieved even if the lesion was in the eloquent area. The patient received postoperative adjuvant radiotherapy (total dose 54 Gy). However, within 1 year postsurgery, the tumor recurred and a second resection surgery was performed. Four years after the first surgery, the tumor metastasized to the spinal cord and the patient eventually died (total postoperative follow-up period was 4 years and 4 months).

Intraoperative histological specimens were immersed in optimal cutting temperature compound (YUAIKASEI, Japan), fixed in liquid nitrogen, and cryosectioned. Intraoperative cytological specimens were either squashed or imprinted and immediately fixed in 95% ethanol prior to hematoxylin and eosin (HE) staining.

Histological findings of the frozen tumor tissue revealed sheet-like proliferation of tumor cells with branching blood vessels (Fig. 1d). Perivascular rosettes were ambiguous, with no apparent fine fibrillary processes in the perivascular anucleate zones (Fig. 1e). Tumor cells exhibited enlarged hyperchromatic nuclei and condensed eosinophilic cytoplasm, giving a rhabdoid appearance (Fig. 1f). In cytological specimens, irregular clusters of tumor cells were observed in the perivascular and nonperivascular areas (Fig. 1g). The tumor cells had oval hyperchromatic nuclei and uniform eosinophilic cytoplasm with fibrillary processes (Fig. 1h). Mitotic cells were observed (Fig. 1h, inset). Whorl formation or intranuclear cytoplasmic pseudo-inclusions were not evident. Additionally, no apparent microvascular proliferation or necrosis was observed. The lesion was intraoperatively diagnosed as a meningioma, suspected to be a rhabdoid meningioma.

In FFPE tissues, high cellularity was observed, with evidence of tumor cell proliferation and branching vessels (Fig. 2a). Perivascular rosettes were present (Fig. 2a), with distinct fine fibrillary processes in the perivascular anucleate zones (Fig. 2b). The tumor cells exhibited oval hyperchromatic nuclei and eosinophilic cytoplasm with a high N/C ratio (Fig. 2b, c), inconspicuous rhabdoid appearance in the cytoplasm, and high mitotic rates (36 mitotic counts per ten high-power fields) (Fig. 2c). Microvascular proliferation (Fig. 2d), necrosis (Fig. 2e), and dural invasion (Fig. 2f) were observed.

Immunohistochemistry was performed as previously reported [14]. The tumor cells were positive for GFAP, EMA (Fig. 2g), and L1CAM (Fig. 2h). The Ki-67 labeling index was 43% (215/500). Collectively, the lesion was histologically confirmed as an anaplastic ependymoma. Details of the primary antibodies used for the immunohistochemistry are provided in Table 2.

A portion of the intraoperative specimen kept at 4 °C was used for ultrastructural analysis [14]. The tumor cells exhibited microlumen formation with microvilli indicating ependymal differentiation, without whorled bundles of intermediate filaments in the cytoplasm (Fig. 2i).

To detect RELA fusion gene, DNA was extracted from five FFPE sections of 5 μm thickness using a blackPREP FFPE DNA kit (Analitik Jena AG, Jena, Germany) [15]. Polymerase chain reaction (PCR) and DNA sequencing assays were performed, and C11orf95-RELA fusion gene was detected (Fig. 3a, b).

We report a case of intraoperatively misdiagnosed rare dura-attached SEAE. The tumor was confirmed as anaplastic ependymoma with dural invasion on the basis of the evaluation of the postoperative histological diagnosis of FFPE specimens. A similar case was reported by Salunke et al. [9] as the intraoperative diagnosis “meningioma, favor” (Table 1). However, details about the intraoperative findings were not provided.

An accurate intraoperative diagnosis for dura-attached SEAEs is often challenging, owing to bias by the preoperative information, insufficient time, and lacking ancillary techniques. In our case, to minimize the effect of freezing/drying artifacts during intraoperative diagnosis [16], tissue was snap-fixed in liquid nitrogen. Nevertheless, perivascular rosettes were inconspicuous owing to obscure fine fibrillary processes in the anucleate zones in the intraoperative frozen sections. In the FFPE tissue, perivascular rosettes were evident, and the tumor cells demonstrated eosinophilic cytoplasm without rhabdoid appearance. Electron microscopic examination revealed no apparent whorled bundles of intermediate filaments, usually seen in rhabdoid meningiomas.

RELA fusion-positive ependymoma is considered the worst prognosis category of the three molecular subgroups of supratentorial ependymoma [3]. However, a few studies have reported favorable outcomes in patients diagnosed with RELA fusion-positive ependymoma and who had undergone with gross total resection [17, 18]. C11orf95-RELA fusion gene was previously reported in one SEAE case with a history of 6-month follow-up and no recurrence [10]. The present case with the same fusion gene detected had two recurrences during the postoperative follow-up period of 1 and 4 years, and the patient died 4 months after the second recurrence. Therefore, the biological significance of the RELA fusion gene as a prognostic factor remains ambiguous in supratentorial ependymoma.

We report a case of dura-attached SEAE misdiagnosed as meningioma on the basis of intraoperative diagnosis using frozen tissue sections and cytology. Neuropathologists should take great precaution in intraoperatively diagnosing this rare subtype of ependymoma to avoid misdiagnosis of the lesion as other common dura-attached tumors.