Skip to main content
main-content

20.07.2019 | Basic Science | Ausgabe 9/2019

Graefe's Archive for Clinical and Experimental Ophthalmology 9/2019

Deciphering the role of circulating lncRNAs: RNCR2, NEAT2, CDKN2B-AS1, and PVT1 and the possible prediction of anti-VEGF treatment outcomes in diabetic retinopathy patients

Zeitschrift:
Graefe's Archive for Clinical and Experimental Ophthalmology > Ausgabe 9/2019
Autoren:
Eman A. Toraih, Ahmed A. Abdelghany, Noha M Abd El Fadeal, Essam Al Ageeli, Manal S. Fawzy
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1007/​s00417-019-04409-9) contains supplementary material, which is available to authorized users.

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Abstract

Purpose

Putative roles of long non-coding RNAs (lncRNAs) as indicators for diabetic retinopathy (DR) and associated complications are beginning to emerge. We aimed to evaluate a panel of circulating hyperglycemia-related lncRNAs: RNCR2, NEAT2, CDKN2B-AS1, and PVT1 in type 2 diabetes patients with/without DR and to correlate their levels with the clinical characteristics and response to aflibercept intravitreal injection in terms of visual acuity (VA) improvement, central macular thickness (CMT) decline, and macular edema resolution after 4 weeks of the initial injection.

Methods

Pre-treatment plasma relative expression levels of the specified lncRNAs were quantified in 130 consecutive patients with diabetes (75 and 55 with/without DR, respectively) and 108 controls using quantitative real-time PCR.

Results

One month after aflibercept injection, significant reductions in CMT and VA were observed in DR cohorts. The four lncRNAs were over-expressed in DM compared with those in controls. However, downregulated baseline plasma levels of RNCR2 and NEAT2 were observed in glycemic-controlled DR patients. None of the lncRNAs showed a correlation with the severity of retinopathy or drug response.

Conclusion

Though circulating levels of the analyzed lncRNAs did not show an association with DR progression or aflibercept therapy response, the expression pattern demonstrated good diagnostic performance in differentiating DM from controls and DR.

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

★ PREMIUM-INHALT
e.Med Interdisziplinär

Mit e.Med Interdisziplinär erhalten Sie Zugang zu allen CME-Fortbildungen und Fachzeitschriften auf SpringerMedizin.de. Zusätzlich können Sie eine Zeitschrift Ihrer Wahl in gedruckter Form beziehen – ohne Aufpreis.

Jetzt e.Med zum Sonderpreis bestellen!

Zusatzmaterial
ESM 1 (DOCX 1516 kb)
417_2019_4409_MOESM1_ESM.docx
ESM 2 (DOCX 33 kb)
417_2019_4409_MOESM2_ESM.docx
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 9/2019

Graefe's Archive for Clinical and Experimental Ophthalmology 9/2019 Zur Ausgabe


 

Neu im Fachgebiet Augenheilkunde

24.09.2019 | Der Ophthalmologe | CME | Ausgabe 10/2019

Konjunktivale Malignome

18.09.2019 | Leitthema | Ausgabe 10/2019

Toxizität von schweren Flüssigkeiten

Bildnachweise