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International Journal of Diabetes in Developing Countries
Erschienen in: International Journal of Diabetes in Developing Countries 1/2014

01.03.2014 | Original Article

Dendritic cells frequency and phenotype in Egyptian type 1 diabetic patients

verfasst von: Asmaa Mohamad Zahran, Khalid Ibrahim Elsayh, Hekma Saad Farghaly

Erschienen in: International Journal of Diabetes in Developing Countries | Ausgabe 1/2014

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Abstract

This study was performed to investigate changes in the dendritic cells (DCs) frequency and phenotype in the peripheral blood in Egyptian Type 1 diabetic children. Also to study the level of B, T lymphocytes, activated T lymphocytes, and costimulatory molecules expression on B lymphocytes. Twenty five children with T1DM and 25 healthy controls were enrolled. Flow cytometric detection of DCs, B-lymphocytes and T-lymphocytes, CD19+CD80+, CD19+CD86+, CD19+HLA-DR+ and CD3+ HLA-DR+ was preformed. The frequencies of monocytoid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs) were significantly decreased in diabetic patients than the controls and the mDCs/pDCs ratio was significantly higher in diabetic patients. The expression of costimulatory molecules CD80 and CD86 on the entire DCs was significantly higher in diabetic children. The frequency of pDCs was negatively correlated with the age in diabetic patients and positively correlated with the level of insulin C-peptide. The percentage of CD80 expressing B lymphocytes and of activated T lymphocytes was significantly higher in the patients. Dendritic cells are reduced in number and display more mature phenotype in T1DM children. The higher expression of CD80 on B lymphocytes and activation of T lymphocytes may reflect the ongoing autoimmune process in this disease. Modulation of the DCs could have beneficial effect in T1DM.
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Metadaten
Titel
Dendritic cells frequency and phenotype in Egyptian type 1 diabetic patients
verfasst von
Asmaa Mohamad Zahran
Khalid Ibrahim Elsayh
Hekma Saad Farghaly
Publikationsdatum
01.03.2014
Verlag
Springer India
Erschienen in
International Journal of Diabetes in Developing Countries / Ausgabe 1/2014
Print ISSN: 0973-3930
Elektronische ISSN: 1998-3832
DOI
https://doi.org/10.1007/s13410-013-0143-1

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