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Journal of Assisted Reproduction and Genetics

29.10.2019 | Commentary

Density gradient centrifugation and swim-up for ICSI: useful, unsafe, or just unsuitable?

verfasst von: Hamilton De Martin, Eduardo P. Miranda, Marcello S. Cocuzza, Pedro A. A. Monteleone

Erschienen in: Journal of Assisted Reproduction and Genetics

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Excerpt

Intracytoplasmic sperm injection (ICSI) revolutionized the treatment of male factor infertility [1]. ICSI eliminates all natural selection barriers, since the spermatozoon is directly injected into the oocyte cytoplasm [2]. While visual inspection permits a trained embryologist to identify immature spermatozoa, the damage in sperm DNA itself does not result in any morphological changes, making its identification nearly impossible [3]. Therefore, semen processing prior to ICSI procedures is of utmost importance. …

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Quinn MM, Jalalian L, Ribeiro S, Ona K, Demirci U, Cedars MI, et al. Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples. Hum Reprod. 2018.

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De Martin H, Cocuzza MS, Tiseo BC, Wood GJA, Miranda EP, Monteleone PAA, et al. Positive rheotaxis extended drop: a one-step procedure to select and recover sperm with mature chromatin for intracytoplasmic sperm injection. J Assist Reprod Genet. 2017;34(12):1699–708.CrossRef

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Titel: Density gradient centrifugation and swim-up for ICSI: useful, unsafe, or just unsuitable?
verfasst von: Hamilton De Martin
Eduardo P. Miranda
Marcello S. Cocuzza
Pedro A. A. Monteleone
Publikationsdatum: 29.10.2019
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-019-01602-x

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Density gradient centrifugation and swim-up for ICSI: useful, unsafe, or just unsuitable?

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Neu im Fachgebiet Gynäkologie und Geburtshilfe

Hochrisiko-Eingriffe für postoperative Übelkeit

Adjuvante Brustkrebstherapie: Doppelt hält besser

Manche Gestagene können das Risiko für Meningeome erhöhen

Stillende Frauen haben geringeres kardiovaskuläres Risiko

Update Gynäkologie