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Erschienen in: International Journal of Legal Medicine 1/2017

17.08.2016 | Original Article

Deposition of diazepam and its metabolites in hair following a single dose of diazepam

verfasst von: Xin Wang, Sys Stybe Johansen, Yurong Zhang, Jingying Jia, Yulan Rao, Fengli Jiang, Kristian Linnet

Erschienen in: International Journal of Legal Medicine | Ausgabe 1/2017

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Abstract

Only sporadic data are available on hair concentrations of diazepam and some of its metabolites (nordazepam, oxazepam, and temazepam) following a single controlled dose. The aim of this study was to investigate the deposition of diazepam and its metabolites in human hair after eight healthy volunteers (four women and four men, ages 24–26, East Asian) consumed 10 mg of diazepam. Hair was collected from all volunteers 1 month after exposure, and also 2 months post-exposure from men and 10 months post-exposure from women. Diazepam and the complete metabolite profile, including oxazepam glucuronide and temazepam glucuronide, were measured by ultra-high pressure liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) with limits of quantifications (LOQs) of 0.5–2.5 pg/mg for diazepam, nordazepam, oxazepam, and temazepam, and of 10 pg/mg for oxazepam glucuronide and temazepam glucuronide. There were no differences by gender in the amounts of diazepam or metabolites found. The concentration of the main metabolite nordazepam was consistently higher than that of diazepam at both 1 and 2 months after consumption. Oxazepam and temazepam traces were found in some volunteers’ hair, but the glucuronides were not detected. Diazepam and nordazepam levels at 10 months post-exposure were extremely low (near the LOQ), indicating drug loss by personal hygiene and physical handling. To our knowledge, this is the first single-dose diazepam study using black hair and the first study to include measurements of oxazepam glucuronide and temazepam glucuronide in human hair.
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Metadaten
Titel
Deposition of diazepam and its metabolites in hair following a single dose of diazepam
verfasst von
Xin Wang
Sys Stybe Johansen
Yurong Zhang
Jingying Jia
Yulan Rao
Fengli Jiang
Kristian Linnet
Publikationsdatum
17.08.2016
Verlag
Springer Berlin Heidelberg
Erschienen in
International Journal of Legal Medicine / Ausgabe 1/2017
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI
https://doi.org/10.1007/s00414-016-1429-x

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