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Journal of Assisted Reproduction and Genetics

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01.12.2014 | Gamete Biology

Depression of HspA2 in human testis is associated with spermatogenic impairment and fertilization rate in ICSI treatment for azoospermic individuals

verfasst von: Yonghong Tian, Fengbin Zhang, Xinzong Zhang, Lejun Li, Liquang Wang, Biwei Shi, Jian Xu

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 12/2014

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Abstract

Purpose

Heat shock protein A2 (HspA2) expression was quantitatively measured in human testis and its relationship with the spermatogenetic status and laboratory outcomes of intracytoplasmic sperm injection (ICSI) was investigated.

Methods

Testicular tissues of azoospermia men were divided into four groups according to histopahtology: normal spermatogenesiss, hypospermatogenesis, maturation arrest and Sertoli cell-only syndrome (SCOS). HspA2 immunostaining was measured by Image Pro-Plus (IPP) and laboratory outcomes were calculated. The regression analysis between HspA2 expression and Johnsen score of as well as fertilization, cleavage and high quality embryo rate was performed.

Results

HspA2 was strongly present in the cytoplasm of spermatocytes and spermatides in normal testis. However, hypospermatogenesis and maturation arrest testicular tissues demonstrated light staining and no staining for SCOS. Quantitative image analysis showed that there were significant differences among groups (P = 0.000 & P = 0.001). HspA2 exspression was founded significantly correlated spermatogenetic status (R² = 0.726, P = 0.000) as well as fertilization rate in ICSI (R² = 0.569, P = 0.000).

Conclusions

The fertilization rate with ICSI is associated with HspA2 expression in the testis from which sperm retrieved and the alteration of HspA2 expression has been involved in spermatogenic impairment.

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Titel: Depression of HspA2 in human testis is associated with spermatogenic impairment and fertilization rate in ICSI treatment for azoospermic individuals
verfasst von: Yonghong Tian
Fengbin Zhang
Xinzong Zhang
Lejun Li
Liquang Wang
Biwei Shi
Jian Xu
Publikationsdatum: 01.12.2014
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 12/2014
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-014-0360-7

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Depression of HspA2 in human testis is associated with spermatogenic impairment and fertilization rate in ICSI treatment for azoospermic individuals

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Purpose

Methods

Results

Conclusions

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Methods

Results

Conclusions

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