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Comparative Clinical Pathology

Erschienen in:

16.08.2017 | Original Article

Detection of Brucella abortus by immunofluorescence assay using anti-16-kDa outer membrane protein (OMP16) antibody

verfasst von: Elham Mohammadi, Mehdi Golchin

Erschienen in: Comparative Clinical Pathology | Ausgabe 6/2017

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Abstract

Brucellosis in humans is one of the most important zoonotic diseases worldwide. This disease causes weakness, fever, pain, sweats, and complications in the liver, spleen, and lymphatic system; Brucellosis requires long-term antibiotic therapy. This study attempts to identify Brucella abortus via an immunofluorescence (IF) test considering antibody against an outer membrane protein with 16-kDa molecular weight (OMP16). The B. abortus OMP16 protein was synthesized through cloning and expressing of the related gene and finally was purified to immunize rabbit in order to produce a polyclonal antibody. Fluorescein isothiocyanate (FITC) was attached to the antibody and the prepared complex was applied to screening of Brucella spp., as an IF test. Polyclonal antibody obtained from the sera of immunized rabbit with the purified OMP16 showed a strong reactivity in ELISA and properly identified the B. abortus strain in IF test. So, this high titer of antibody could be helpful for tracing of B. abortus via laboratory diagnostic tests.

Baldi PC, Giambartolomei GH, Goldbaum FA, Abdon LF, Velikovsky CA, Kittelberger R, Fossati CA (1996) Humoral immune response against lipopolysaccharide and cytoplasmic proteins of Brucella abortus in cattle vaccinated with B. abortus S19 or experimentally infected with Yersinia enterocolitica serotype 0: 9. Clin Diagn Lab Immunol 3:472–476PubMedPubMedCentral

Cassataro J et al (2007) A DNA vaccine coding for the chimera BLSOmp31 induced a better degree of protection against B. ovis and a similar degree of protection against B. melitensis than Rev. 1 vaccination. Vaccine 25:5958–5967CrossRefPubMed

Christopher S, Umapathy B, Ravikumar K (2010) Brucellosis: review on the recent trends in pathogenicity and laboratory diagnosis. J Lab Physicians 2:55CrossRefPubMedPubMedCentral

Cloeckaert A, Baucheron S, Vizcaino N, Zygmunt MS (2001) Use of recombinant BP26 protein in serological diagnosis of Brucella melitensis infection in sheep. Clin Diagn Lab Immunol 8:772–775PubMedPubMedCentral

Cloeckaert A, De Wergifosse P, Dubray G, Limet J (1990) Identification of seven surface-exposed Brucella outer membrane proteins by use of monoclonal antibodies: immunogold labeling for electron microscopy and enzyme-linked immunosorbent assay. Infect Immun 58:3980–3987PubMedPubMedCentral

Cloeckaert A, Jacques I, Bosseray N, Limet J, Bowden R, Dubray G, Plommet M (1991) Protection conferred on mice by monoclonal antibodies directed against outer-membrane-protein antigens of Brucella. J Med Microbiol 34:175–180CrossRefPubMed

Cloeckaert C, Jacques I, Limet J, Dubray G (1995) Immunogenic properties of Brucella melitensis cell-wall fractions in BALB/c mice. J Med Microbiol 42:200–208CrossRefPubMed

Corbel M (1997) Recent advances in brucellosis. J Med Microbiol 46:101–103CrossRefPubMed

de Bagüés MJ, Elzer P, Blasco J, Marin C, Gamazo C, Winter A (1994) Protective immunity to Brucella ovis in BALB/c mice following recovery from primary infection or immunization with subcellular vaccines. Infect Immun 62:632–638

de Bagüés MJ, Marin C, Blasco J, Moriyon I, Gamazo C (1992) An ELISA with Brucella lipopolysaccharide antigen for the diagnosis of B. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival B. melitensis strain Rev 1 vaccination. Vet Microbiol 30:233–241CrossRef

de Wergifosse P, Lintermans P, Limet JN, Cloeckaert A (1995) Cloning and nucleotide sequence of the gene coding for the major 25-kilodalton outer membrane protein of Brucella abortus. J Bacteriol 177:1911–1914CrossRefPubMedPubMedCentral

Delpino MV, Estein SM, Fossati CA, Baldi PC, Cassataro J (2007) Vaccination with Brucella recombinant DnaK and SurA proteins induces protection against Brucella abortus infection in BALB/c mice. Vaccine 25:6721–6729CrossRefPubMed

Dubray G (1973) Le peptidoglycane des Brucella: mise en évidence d’une structure atriple feuillet. C R Acad Sci Paris 277:2281–2283

Dubray G, Bezard G (1980) Isolation of three Brucella abortus cell-wall antigens protective in murine experimental brucellosis. Ann Rech Vet 11:367–373PubMed

Eskra L, Canavessi A, Carey M, Splitter G (2001) Brucella abortus genes identified following constitutive growth and macrophage infection. Infect Immun 69:7736–7742CrossRefPubMedPubMedCentral

Farshad S, Mehrabanpour M, Namavari M, Hosseini S, Tavakkoli A, Ghaderi A (2002) Production and characterization of monoclonal antibodies against Brucella abortus S (99) surface antigens. Iran Biomed J 6:7–12

Ficht TA, Bearden S, Sowa B, Adams L (1988) A 36-kilodalton Brucella abortus cell envelope protein is encoded by repeated sequences closely linked in the genomic DNA. Infect Immun 56:2036–2046PubMedPubMedCentral

Ghasemi A, Salari MH, Zarnani AH, Pourmand MR, Ahmadi H, Mirshafiey A, Jeddi-Tehrani M (2013) Immune reactivity of Brucella melitensis-vaccinated rabbit serum with recombinant Omp31 and DnaK proteins. I J M 5:19–23

Giambartolomei GH, Zwerdling A, Cassataro J, Bruno L, Fossati CA, Philipp MT (2004) Lipoproteins, not lipopolysaccharide, are the key mediators of the proinflammatory response elicited by heat-killed Brucella abortus. J Immunol 173:4635–4642CrossRefPubMed

Gomez-Miguel MJ, Moriyon I (1986) Demonstration of a peptidoglycan-linked lipoprotein and characterization of its trypsin fragment in the outer membrane of Brucella spp. Infect Immun 53:678–684PubMedPubMedCentral

Gomez-Miguel MJ, Moriyon I, Lopez J (1987) Brucella outer membrane lipoprotein shares antigenic determinants with Escherichia coli Braun lipoprotein and is exposed on the cell surface. Infect Immun 55:258–262PubMedPubMedCentral

Gomez G, Pei J, Mwangi W, Adams LG, Rice-Ficht A, Ficht TA (2013) Immunogenic and invasive properties of Brucella melitensis 16M outer membrane protein vaccine candidates identified via a reverse vaccinology approach. PLoS One 8:e59751CrossRefPubMedPubMedCentral

Gupta V, Kumari R, Vohra J, Singh S, Vihan V (2010) Comparative evaluation of recombinant BP26 protein for serological diagnosis of Brucella melitensis infection in goats. Small Ruminant Res 93:119–125CrossRef

Ibañez AE et al. (2013) Unlipidated outer membrane protein Omp16 (U-Omp16) from Brucella spp. as nasal adjuvant induces a Th1 immune response and modulates the Th2 allergic response to cow’s milk proteins. PloS one 8:e69438

Limet J, Cloeckaert A, Bezard G, Van Broeck J, Dubray G (1993) Antibody response to the 89-kDa outer membrane protein of Brucella in bovine brucellosis. J Med Microbiol 39:403–407CrossRefPubMed

Lindler LE et al (1996) Cloning of a Brucella melitensis group 3 antigen gene encoding Omp28, a protein recognized by the humoral immune response during human brucellosis. Infect Immun 64:2490–2499PubMedPubMedCentral

Morgan W, MacKinnon D, Lawson J, Cullen G (1969) The rose bengal plate agglutination test in the diagnosis of brucellosis. Vet Rec 85:636–641CrossRefPubMed

Munir R, Afzal M, Hussain M, Naqvi S, Khanum A (2010) Outer membrane proteins of Brucella abortus vaccinal and field strains and their immune response in buffaloes. Pak Vet J 30:110–114

Pasquevich KA et al (2009) Immunization with recombinant Brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD4+ and CD8+ T cells as well as systemic and oral protection against Brucella abortus infection. Infect Immun 77:436–445CrossRefPubMed

Pasquevich KA et al (2010) The protein moiety of Brucella abortus outer membrane protein 16 is a new bacterial pathogen-associated molecular pattern that activates dendritic cells in vivo, induces a Th1 immune response, and is a promising self-adjuvanting vaccine against systemic and oral acquired brucellosis. J Immunol 184:5200–5212CrossRefPubMed

Raybould T (1982) Antigens of diagnostic significance in Brucella abortus. Can J Microbiol 28:557–566CrossRefPubMed

Riezu-Boj JI, Moriyon I, Blasco J, Gamazo C, Diaz R (1990) Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis. Infect Immun 58:489–494PubMedPubMedCentral

Ruiz-Mesa J, Sánchez-Gonzalez J, Reguera J, Martin L, Lopez-Palmero S, Colmenero J (2005) Rose Bengal test: diagnostic yield and use for the rapid diagnosis of human brucellosis in emergency departments in endemic areas. Clin Microbiol Infect 11:221–225CrossRefPubMed

Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Coldspring-Harbour Laboratory Press, New York

Smirnova EA et al (2013) Current methods of human and animal brucellosis diagnostics. Adv Infec Dis 3:177–184

Sowa B, Kelly K, Ficht T, Frey M, Adams L (1991) SDS-soluble and peptidoglycan-bound proteins in the outer membrane-peptidoglycan complex of Brucella abortus. Vet Microbiol 27:351–369CrossRefPubMed

Sung KY, Jung M, Shin M-K, Park H-E, Lee JJ, Kim S, Yoo HS (2014) Induction of immune responses by two recombinant proteins of brucella abortus, outer membrane proteins 2b porin and Cu/Zn superoxide dismutase, in mouse model. J Microbiol Biotechnol 24:854–861CrossRefPubMed

Tabynov K et al (2014) Influenza viral vectors expressing the Brucella OMP16 or L7/L12 proteins as vaccines against B. abortus infection. Virol J 11:1CrossRef

Tibor A, Decelle B, Letesson J-J (1999) Outer membrane proteins Omp10, Omp16, and Omp19 of Brucella spp. are lipoproteins. Infect Immun 67:4960–4962PubMedPubMedCentral

Verstreate D, Creasy M, Caveney N, Baldwin C, Blab M, Winter A (1982) Outer membrane proteins of Brucella abortus: isolation and characterization. Infect Immun 35:979–989PubMedPubMedCentral

Watarai M et al (2007) A rapid agglutination assay for canine brucellosis using antigen coated beads. J Vet Med Sci 69:477–480CrossRefPubMed

Zheng W, Wang Y, Zhang Z, Yan F (2015) Immunological characteristics of outer membrane protein omp31 of goat Brucella and its monoclonal antibody. Genet Mol Res 14:11965CrossRefPubMed

Titel: Detection of Brucella abortus by immunofluorescence assay using anti-16-kDa outer membrane protein (OMP16) antibody
verfasst von: Elham Mohammadi
Mehdi Golchin
Publikationsdatum: 16.08.2017
Verlag: Springer London
Erschienen in: Comparative Clinical Pathology / Ausgabe 6/2017
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-017-2529-9

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