Detection of glioblastoma multiforme using quantitative molecular magnetic resonance imaging based on 5-aminolevulinic acid: in vitro and in vivo studies
- 08.12.2021
- Research Article
Erschienen in:
- Verfasst von
- Anita Ebrahimpour
- Fatemeh Tirgar
- Behnam Hajipour-Verdom
- Ardeshir Abbasi
- Mahmoudreza Hadjighassem
- Parviz Abdolmaleki
- Saereh Hosseindoost
- Seyed Amir Hossein Javadi
- Hassan Hashemi
- Abbas Rahimi Foroushani
- Nader Riyahi Alam
- Mehdi Khoobi
Abstract
Objectives
We demonstrated a novel metabolic method based on sequential administration of 5-aminolevulinic acid (ALA) and iron supplement, and ferric ammonium citrate (FAC), for glioblastoma multiforme (GBM) detection using R2’ and quantitative susceptibility mapping (QSM).
Materials and methods
Intra-cellular iron accumulation in glioblastoma cells treated with ALA and/or FAC was measured. Cell phantoms containing glioblastoma cells and Wistar rats bearing C6 glioblastoma were imaged using a 3 T MRI scanner after sequential administration of ALA and FAC. The relaxivity and QSM analysis were performed on the images.
Results
The intra-cellular iron deposition was significantly higher in the glioma cells with sequential treatment of ALA and FAC for 6 h compared to those treated with the controls. The relaxivity and magnetic susceptibility values of the glioblastoma cells and rat brain tumors treated with ALA + FAC (115 ± 5 s-1 for R2’, and 0.1 ± 0.02 ppm for magnetic susceptibility) were significantly higher than those treated with the controls (55 ± 18 (FAC), 45 ± 15 (ALA) s-1 for R2’, p < 0.05, and 0.03 ± 0.03 (FAC), 0.02 ± 0.02 (ALA) ppm for magnetic susceptibility, p < 0.05).
Discussion
Sequential administration of ALA and iron supplements increases the iron deposition in glioblastoma cells, enabling clinical 3 T MRI to detect GBM using R2’ or QSM.
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- Titel
- Detection of glioblastoma multiforme using quantitative molecular magnetic resonance imaging based on 5-aminolevulinic acid: in vitro and in vivo studies
- Verfasst von
-
Anita Ebrahimpour
Fatemeh Tirgar
Behnam Hajipour-Verdom
Ardeshir Abbasi
Mahmoudreza Hadjighassem
Parviz Abdolmaleki
Saereh Hosseindoost
Seyed Amir Hossein Javadi
Hassan Hashemi
Abbas Rahimi Foroushani
Nader Riyahi Alam
Mehdi Khoobi
- Publikationsdatum
- 08.12.2021
- Verlag
- Springer International Publishing
- Erschienen in
-
Magnetic Resonance Materials in Physics, Biology and Medicine / Ausgabe 1/2022
Print ISSN: 0968-5243
Elektronische ISSN: 1352-8661 - DOI
- https://doi.org/10.1007/s10334-021-00978-1
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