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07.11.2019 | Original Contribution

Detection of Leptospira spp. in Captive Broad-Snouted Caiman (Caiman latirostris)

verfasst von: Lucas Nogueira Paz, Camila Hamond, Carla Silva Dias, Vitor P. Curvelo, Marco Alberto Medeiros, Arianne Pontes Oriá, Melissa Hanzen Pinna

Erschienen in: EcoHealth | Ausgabe 4/2019

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Abstract

Leptospira sp. is an important waterborne zoonotic bacterium, known to cause infection in animals and humans worldwide. The role of reptiles in the transmission of this microorganism is poorly understood and historically neglected. This study aimed to investigate the presence of anti-Leptospira spp. antibodies and leptospiral DNA in captive Caiman latirostris (broad-snouted caiman). Of the 23 reptiles studied by microscopic agglutination test (MAT), 22/23 (95.65%) were considered reactive (titers ≥ 100) and 1/23 (4.35%) non-reactive (titer < 100). The serogroup with highest occurrence was Grippotyphosa (68.18%, n = 15/22) followed by serogroup Djasiman (18.18%, n = 4/22). Specific amplification of Leptospira spp. gene lipL32 was observed in six (26.09%, n = 6/23) blood samples. Five of six samples, previously detected as pathogenic leptospira by PCR, were amplified and sequenced. All the samples corresponded to the pathogenic species Leptospira interrogans (presented 100% of identity) using the PCR targeting to secY gene. We demonstrated high detection of DNA of L. interrogans in crocodilians, and the authors suggest that further research is needed to elucidate the impact of Leptospira spp. infection in health broad-snouted caimans as well as the pathophysiology of leptospirosis in crocodilians.

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Titel: Detection of Leptospira spp. in Captive Broad-Snouted Caiman (Caiman latirostris)
verfasst von: Lucas Nogueira Paz
Camila Hamond
Carla Silva Dias
Vitor P. Curvelo
Marco Alberto Medeiros
Arianne Pontes Oriá
Melissa Hanzen Pinna
Publikationsdatum: 07.11.2019
Verlag: Springer US
Erschienen in: EcoHealth / Ausgabe 4/2019
Print ISSN: 1612-9202
Elektronische ISSN: 1612-9210
DOI: https://doi.org/10.1007/s10393-019-01452-0

Springer Medizin

Abstract

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