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Erschienen in: Journal of Parasitic Diseases 4/2016

13.01.2016 | Original Article

Detection of Toxoplasma gondii in small ruminants in Chennai using PCR and modified direct agglutination test

verfasst von: Ajay Suryakant Satbige, M. Vijaya Bharathi, P. I. Ganesan, C. Sreekumar, C. Rajendran

Erschienen in: Journal of Parasitic Diseases | Ausgabe 4/2016

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Abstract

A total of 193 sera samples, along with tissues (lung, heart, and brain) collected from 136 sheep and 57 goats from the Corporation slaughter house, Madras Veterinary College teaching hospital, and private mutton shops from Chennai were tested for Toxoplasma gondii. All the sera samples were tested using modified direct agglutination test. Of the 193 sera samples, 57 (29.5 %) had a minimum titre of 1:20, with 30.14 % (41/136) of sheep and 28.07 % (16/57) of goats being seropositive. Tissue samples from all 193 animals, when subjected to B1 based PCR to detect T. gondii DNA, showed 3.67 and 3.50 % to be positive in sheep and goats, respectively. In the present investigation B1 based PCR detected T. gondii in low numbers, possibly due to limitation of the sample size. The presence of T. gondii in tissues of sheep and goats slaughtered for human consumption in Chennai indicates the role of these food animals as potential sources of infection to human.
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Metadaten
Titel
Detection of Toxoplasma gondii in small ruminants in Chennai using PCR and modified direct agglutination test
verfasst von
Ajay Suryakant Satbige
M. Vijaya Bharathi
P. I. Ganesan
C. Sreekumar
C. Rajendran
Publikationsdatum
13.01.2016
Verlag
Springer India
Erschienen in
Journal of Parasitic Diseases / Ausgabe 4/2016
Print ISSN: 0971-7196
Elektronische ISSN: 0975-0703
DOI
https://doi.org/10.1007/s12639-015-0713-x

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