Erschienen in:
21.03.2016 | Brief Report
Detection of West Nile virus and tick-borne encephalitis virus in birds in Slovakia, using a universal primer set
verfasst von:
Tomáš Csank, Katarína Bhide, Elena Bencúrová, Saskia Dolinská, Petra Drzewnioková, Peter Major, Ľuboš Korytár, Eva Bocková, Mangesh Bhide, Juraj Pistl
Erschienen in:
Archives of Virology
|
Ausgabe 6/2016
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Abstract
West Nile virus (WNV) is a mosquito-borne neurotropic pathogen that presents a major public health concern. Information on WNV prevalence and circulation in Slovakia is insufficient. Oral and cloacal swabs and bird brain samples were tested for flavivirus RNA by RT-PCR using newly designed generic primers. The species designation was confirmed by sequencing. WNV was detected in swab and brain samples, whereas one brain sample was positive for tick-borne encephalitis virus (TBEV). The WNV sequences clustered with lineages 1 and 2. These results confirm the circulation of WNV in birds in Slovakia and emphasize the risk of infection of humans and horses.