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12.11.2021 | Original Article

Development and validation of a sensitive and high throughput UPLC–MS/MS method for determination of paraquat and diquat in human plasma and urine: application to poisoning cases at emergency departments of hospitals

verfasst von: Zhengsheng Mao, Youjia Yu, Hao Sun, Weirang Xie, Hongmei Zhao, Qiaoyan Jiang, Yang Sun, Yue Cao, Feng Chen

Erschienen in: Forensic Toxicology | Ausgabe 1/2022

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Abstract

Purpose

Paraquat and diquat are well-known toxic herbicides, at least responsible for hundreds of fatal poisoning events worldwide. However, the determination of diquat and paraquat in plasma and urine is very challenging because of their high polarity and double charge characteristics. In this study, we aim to develop a rapid and reliable method for the determination of paraquat and diquat in human plasma and urine by ultraperformance liquid chromatography–tandem mass spectrometry.

Method

The chromatographic separation of paraquat and diquat was tested with different chromatographic columns and different mobile phase conditions. The mass parameters were optimized by product ions, source gas flow, cone flow, desolvation temperature, and capillary voltage. The isocratic elution mode gave rapid appearance of peak of paraquat and diquat.

Results

The sharp peak shapes for paraquat and diquat were achieved with CORTECS^® UPLC^® HILIC (100 × 2.1 mm, 1.6 μm) column by adding formic acid and ammonium acetate in mobile phase in isocratic elution mode. The lower limit of quantification of 1.0 ng/mL for paraquat and diquat were achieved using only 50 μL of human plasma or urine. The running time for analysis of both paraquat and diquat was as short as 3.5 min per sample.

Conclusions

A rapid and reliable method for the determination of paraquat and diquat was developed and applied to 387 clinical poisoning cases and 22 poisoning cases were found to be paraquat or diquat poisoning.

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Titel: Development and validation of a sensitive and high throughput UPLC–MS/MS method for determination of paraquat and diquat in human plasma and urine: application to poisoning cases at emergency departments of hospitals
verfasst von: Zhengsheng Mao
Youjia Yu
Hao Sun
Weirang Xie
Hongmei Zhao
Qiaoyan Jiang
Yang Sun
Yue Cao
Feng Chen
Publikationsdatum: 12.11.2021
Verlag: Springer Singapore
Erschienen in: Forensic Toxicology / Ausgabe 1/2022
Print ISSN: 1860-8965
Elektronische ISSN: 1860-8973
DOI: https://doi.org/10.1007/s11419-021-00603-9

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01.04.2024 | Forensische Traumatologie | CME

Springer Medizin

Development and validation of a sensitive and high throughput UPLC–MS/MS method for determination of paraquat and diquat in human plasma and urine: application to poisoning cases at emergency departments of hospitals

Abstract

Purpose

Method

Results

Conclusions

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Abstract

Purpose

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Results

Conclusions

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