nach oben

Erschienen in:

21.02.2018 | Original Article

Development of a nanogold slot blot inhibition assay for the detection of antibodies against bovine herpesvirus type 1

verfasst von: Greice Japolla, Jair Pereira Cunha-Junior, Ana Claudia Arantes Marquez Pajuaba, Ernesto Akio Taketomi, Samira Bührer-Sékula, Luiz Artur Mendes Bataus, Guilherme Rocha Lino de Souza

Erschienen in: Archives of Virology | Ausgabe 6/2018

Einloggen, um Zugang zu erhalten

Abstract

Bovine herpesvirus type 1 (BoHV-1) is recognized as an important pathogen causing respiratory, reproductive, and neurological disorders in cattle and is associated with economic losses to animal industry. Accurate diagnostic methods are needed for prevention of disease transmission. While the virus neutralization test is considered the gold standard method, it requires maintenance of the virus and cell cultures, which is time consuming and expensive. Serological techniques such as enzyme-linked immunosorbent assay (ELISA) are widely applied, as these are easy to perform and provide quick results. In the present study, a nanogold slot blot inhibition assay was developed for the serological diagnosis of BoHV-1 and compared with standard ELISA and horseradish peroxidase (HRP) slot blot assays. Of 42 serum samples tested by ELISA, 32 (76.2%) were positive and 10 (23.8%), were negative. The sensitivity and specificity of the nanogold slot blot inhibition assay was similar to that observed for ELISA and HRP slot blot assays, and a strong correlation was observed between the tests. Thus, the nanogold slot blot inhibition assay may serve as an efficient and rapid alternative to ELISA in settings, where plate-reading equipment is lacking.

Nur mit Berechtigung zugänglich

Marin MS, Leunda MR, Verna AE, Morán PE, Odeón AC, Pérez SE (2016) Distribution of bovine herpesvirus type 1 in the nervous system of experimentally infected calves. Vet J 209:82–86. https://doi.org/10.1016/j.tvjl.2015.10.034 CrossRefPubMed

Rissi DR, Pierezan F, Sa e Silva M, Flores EF, de Barros CS (2008) Neurological disease in cattle in southern Brazil associated with Bovine herpesvirus infection. J Vet Diagn Investig 20(3):346–349. https://doi.org/10.1177/104063870802000315 CrossRef

Engels M, Ackermann M (1996) Pathogenesis of ruminant herpesvirus infections. Vet Microbiol 53(1–2):3–15CrossRefPubMed

Fulton RW (2009) Bovine respiratory disease research (1983–2009). Anim Health Res Rev 10(2):131–139. https://doi.org/10.1017/s146625230999017x CrossRefPubMed

Takiuchi E, Alfieri AF, Alfieri AA (2001) Bovine herpervirus type 1: infection and diagnosis methods. Semina: Ciências Agrarias 22(2):203–209

Mahajan V, Banga HS, Deka D, Filia G, Gupta A (2013) Comparison of diagnostic tests for diagnosis of infectious bovine rhinotracheitis in natural cases of bovine abortion. J Comp Pathol 149(4):391–401. https://doi.org/10.1016/j.jcpa.2013.05.002 CrossRefPubMed

van Drunen Littel-van den Hurk S (2006) Rationale and perspectives on the success of vaccination against bovine herpesvirus-1. Vet Microbiol 113(3–4):275–282. https://doi.org/10.1016/j.vetmic.2005.11.002 CrossRefPubMed

van Oirschot JT, Kaashoek MJ, Rijsewijk FA, Stegeman JA (1996) The use of marker vaccines in eradication of herpesviruses. J Biotechnol 44(1–3):75–81. https://doi.org/10.1016/0168-1656(95)00129-8 CrossRefPubMed

Raaperi K, Orro T, Viltrop A (2015) Effect of vaccination against bovine herpesvirus 1 with inactivated gE-negative marker vaccines on the health of dairy cattle herds. Prev Vet Med 118(4):467–476. https://doi.org/10.1016/j.prevetmed.2015.01.014 CrossRefPubMed

10.

Tignon M, De Baere M, Hanon JB, Goolaerts A, Houtain JY, Delooz L, Cay AB (2017) Characterization of three commercial ELISA kits for detection of BOHV-1 gE specific antibodies in serum and milk samples and applicability of bulk milk for determination of herd status. J Virol Methods 245:66–72. https://doi.org/10.1016/j.jviromet.2017.03.015 CrossRefPubMed

11.

Muratore E, Bertolotti L, Nogarol C, Caruso C, Lucchese L, Iotti B, Ariello D, Moresco A, Masoero L, Nardelli S, Rosati S (2017) Surveillance of infectious bovine rhinotracheitis in marker-vaccinated dairy herds: application of a recombinant gE ELISA on bulk milk samples. Vet Immunol Immunopathol 185:1–6. https://doi.org/10.1016/j.vetimm.2017.01.003 CrossRefPubMed

12.

Walz PH, Givens MD, Rodning SP, Riddell KP, Brodersen BW, Scruggs D, Short T, Grotelueschen D (2017) Evaluation of reproductive protection against bovine viral diarrhea virus and bovine herpesvirus-1 afforded by annual revaccination with modified-live viral or combination modified-live/killed viral vaccines after primary vaccination with modified-live viral vaccine. Vaccine 35(7):1046–1054. https://doi.org/10.1016/j.vaccine.2017.01.006 CrossRefPubMed

13.

Cortese VS, Woolums A, Hurley DJ, Berghaus R, Bernard JK, Short TH (2017) Comparison of interferon and bovine herpesvirus-1-specific IgA levels in nasal secretions of dairy cattle administered an intranasal modified live viral vaccine prior to calving or on the day of calving. Vet Immunol Immunopathol 187:35–41. https://doi.org/10.1016/j.vetimm.2017.04.003 CrossRefPubMed

14.

Silva MS, Brum MCS, Loreto ELS, Weiblen R, Flores EF (2007) Molecular and antigenic characterization of Brazilian bovine herpesvirus type 1 isolates recovered from the brain of cattle with neurological disease. Virus Res 129(2):191–199. https://doi.org/10.1016/j.virusres.2007.07.014 CrossRefPubMed

15.

Marin MS, Leunda MR, Verna AE, Faverín C, Pérez SE, Odeón AC (2012) In vitro replication of bovine herpesvirus types 1 and 5. J Virol Methods 181(1):80–85. https://doi.org/10.1016/j.jviromet.2012.01.016 CrossRefPubMed

16.

House JA, Baker JA (1971) Bovine herpesvirus IBR-IPV. The antibody virus neutralization reaction. Cornell Vet 61(2):320–335PubMed

17.

Choudhary N, Roy A, Leon MG, Wei G, Nakhla MK, Levy L, Brlansky RH (2017) Production of mono- and polyclonal antibodies to Citrus leprosis virus C2 and their application in triple antibody sandwich ELISA and immunocapture RT-PCR diagnostic assays. J Virol Methods 243:177–181. https://doi.org/10.1016/j.jviromet.2017.02.012 CrossRefPubMed

18.

Hauck TS, Giri S, Gao Y, Chan WCW (2010) Nanotechnology diagnostics for infectious diseases prevalent in developing countries. Adv Drug Deliv Rev 62(4–5):438–448. https://doi.org/10.1016/j.addr.2009.11.015 CrossRefPubMed

19.

Wang Y, Wang L, Zhang J, Wang G, Chen W, Chen L, Zhang X (2014) Preparation of colloidal gold immunochromatographic strip for detection of Paragonimiasis skrjabini. PLoS One 9(3):e92034. https://doi.org/10.1371/journal.pone.0092034 CrossRefPubMedPubMedCentral

20.

Ju Y, Hao H-J, Xiong G-H, Geng H-R, Zheng Y-L, Wang J, Cao Y, Yang Y-H, Cai X-H, Jiang Y-Q (2010) Development of colloidal gold-based immunochromatographic assay for rapid detection of Streptococcus suis serotype 2. Vet Immunol Immunopathol 133(2–4):207–211. https://doi.org/10.1016/j.vetimm.2009.08.010 CrossRefPubMed

21.

Bastús NG, Comenge J, Puntes V (2011) Kinetically controlled seeded growth synthesis of citrate-stabilized gold nanoparticles of up to 200 nm: size focusing versus ostwald ripening. Langmuir 27(17):11098–11105. https://doi.org/10.1021/la201938u CrossRefPubMed

22.

Tram DT, Wang H, Sugiarto S, Li T, Ang WH, Lee C, Pastorin G (2016) Advances in nanomaterials and their applications in point of care (POC) devices for the diagnosis of infectious diseases. Biotechnol Adv 34(8):1275–1288. https://doi.org/10.1016/j.biotechadv.2016.09.003 CrossRefPubMed

23.

Wen-de W, Min L, Ming C, Li-ping L, Rui W, Hai-lan C, Fu-Yan C, Qiang M, Wan-wen L, Han-zhong C (2017) Development of a colloidal gold immunochromatographic strip for rapid detection of Streptococcus agalactiae in tilapia. Biosens Bioelectron 91:66–69. https://doi.org/10.1016/j.bios.2016.11.038 CrossRefPubMed

24.

Meng K, Sun W, Zhao P, Zhang L, Cai D, Cheng Z, Guo H, Liu J, Yang D, Wang S, Chai T (2014) Development of colloidal gold-based immunochromatographic assay for rapid detection of Mycoplasma suis in porcine plasma. Biosens Bioelectron 55:396–399. https://doi.org/10.1016/j.bios.2013.12.048 CrossRefPubMed

25.

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefPubMed

26.

Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nat Phys Sci 241:20–22CrossRef

27.

Bright RM, Musick MD, Natan MJ (1998) Preparation and characterization of Ag colloid monolayers. Langmuir 14(20):5695–5701. https://doi.org/10.1021/la980138j CrossRef

28.

Santos VO, Pelegrini PB, Mulinari F, Moura RS, Cardoso LPV, Buhrer-Sekula S, Miller RNG, Pinto ERC, Grossi-de-Sa MF (2015) A novel immunochromatographic strip test for rapid detection of Cry1Ac and Cry8Ka5 proteins in genetically modified crops. Anal Methods 7(21):9331–9339. https://doi.org/10.1039/C5AY02051D CrossRef

29.

Greiner M (1996) Two-graph receiver operating characteristic (TG-ROC): update version supports optimisation of cut-off values that minimise overall misclassification costs. J Immunol Methods 191(1):93–94CrossRefPubMed

30.

Akobeng AK (2007) Understanding diagnostic tests 1: sensitivity, specificity and predictive values. Acta Paediatr (Oslo, Norway: 1992) 96(3):338–341. https://doi.org/10.1111/j.1651-2227.2006.00180.x CrossRef

31.

van Stralen KJ, Stel VS, Reitsma JB, Dekker FW, Zoccali C, Jager KJ (2009) Diagnostic methods I: sensitivity, specificity, and other measures of accuracy. Kidney Int 75(12):1257–1263. https://doi.org/10.1038/ki.2009.92 CrossRefPubMed

32.

Medeiros LS, Carvalho YK, Maciel RCG, Lilenbaum W (2016) Análise de custo-efetividade de protocolos no diagnóstico da tuberculose bovina em um rebanho naturalmente infectado. Pesquisa Veterinária Brasileira 36:485–491CrossRef

33.

Komorowski M, Raffa J (2016) Markov models and cost effectiveness analysis: applications in medical research. In: Secondary analysis of electronic health records, edn. Springer International Publishing, Cham, Swizerland, pp. 351–367. https://doi.org/10.1007/978-3-319-43742-2_24

34.

Raaperi K, Bougeard S, Aleksejev A, Orro T, Viltrop A (2012) Association of herd BRSV and BHV-1 seroprevalence with respiratory disease and reproductive performance in adult dairy cattle. Acta Vet Scand 54:4. https://doi.org/10.1186/1751-0147-54-4 CrossRefPubMedPubMedCentral

35.

Statham JME, Randall LV, Archer SC (2015) Reduction in daily milk yield associated with subclinical bovine herpesvirus 1 infection. Vet Rec 177(13):339. https://doi.org/10.1136/vr.103105 CrossRefPubMed

36.

Sayers RG, Byrne N, O’Doherty E, Arkins S (2015) Prevalence of exposure to bovine viral diarrhoea virus (BVDV) and bovine herpesvirus-1 (BoHV-1) in Irish dairy herds. Res Vet Sci 100:21–30. https://doi.org/10.1016/j.rvsc.2015.02.011 CrossRefPubMed

37.

Rola J, Larska M, Socha W, Rola JG, Materniak M, Urban-Chmiel R, Thiry E, Żmudziński JF (2017) Seroprevalence of bovine herpesvirus 1 related alphaherpesvirus infections in free-living and captive cervids in Poland. Vet Microbiol 204:77–83. https://doi.org/10.1016/j.vetmic.2017.04.006 CrossRefPubMed

38.

Raaperi K, Orro T, Viltrop A (2014) Epidemiology and control of bovine herpesvirus 1 infection in Europe. Vet J 201(3):249–256. https://doi.org/10.1016/j.tvjl.2014.05.040 CrossRefPubMed

39.

Jones C, Geiser V, Henderson G, Jiang Y, Meyer F, Perez S, Zhang Y (2006) Functional analysis of bovine herpesvirus 1 (BHV-1) genes expressed during latency. Vet Microbiol 113(3–4):199–210. https://doi.org/10.1016/j.vetmic.2005.11.009 CrossRefPubMed

40.

Pierotti S, Albano C, Milandri A, Callegari F, Poletti R, Rossini GP (2007) A slot blot procedure for the measurement of yessotoxins by a functional assay. Toxicon 49(1):36–45. https://doi.org/10.1016/j.toxicon.2006.09.008 CrossRefPubMed

41.

Dias JA, Alfieri AA, Ferreira-Neto JS, Goncalves VS, Muller EE (2013) Seroprevalence and risk factors of bovine herpesvirus 1 infection in cattle herds in the state of Parana, Brazil. Transbound Emerg Dis 60(1):39–47. https://doi.org/10.1111/j.1865-1682.2012.01316.x CrossRefPubMed

42.

Zhu D, Saul AJ, Miles AP (2005) A quantitative slot blot assay for host cell protein impurities in recombinant proteins expressed in E. coli. J Immunol Methods 306(1–2):40–50. https://doi.org/10.1016/j.jim.2005.07.021 CrossRefPubMed

43.

Pawar SS, Meshram CD, Singh NK, Saini M, Mishra BP, Gupta PK (2014) Development of a SYBR Green I based duplex real-time PCR for detection of bovine herpesvirus-1 in semen. J Virol Methods 208:6–10. https://doi.org/10.1016/j.jviromet.2014.07.027 CrossRefPubMed

44.

Marin MS, Quintana S, Leunda MR, Recavarren M, Pagnuco I, Späth E, Pérez S, Odeón A (2016) A new method for simultaneous detection and discrimination of bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) using real time PCR with high resolution melting (HRM) analysis. J Virol Methods 227:14–22. https://doi.org/10.1016/j.jviromet.2015.10.005 CrossRefPubMed

45.

Casarin E, Lucchese L, Grazioli S, Facchin S, Realdon N, Brocchi E, Morpurgo M, Nardelli S (2016) A new ELISA using the ANANAS technology showing high sensitivity to diagnose the bovine rhinotracheitis from individual sera to pooled milk. PLoS One 11(1):e0145912CrossRefPubMedPubMedCentral

46.

Al-Yousif Y, Anderson J, Chard-Bergstrom C, Kapil S (2002) Development, evaluation, and application of lateral-flow immunoassay (immunochromatography) for detection of rotavirus in bovine fecal samples. Clin Diagn Lab Immunol 9(3):723–724. https://doi.org/10.1128/CDLI.9.3.723-724.2002 PubMedPubMedCentralCrossRef

47.

Zhang H-C, Liu C-Y, Liu G-Y, Chen X-L, Ye Y-D, Chai C-Y, Wang Y-R (2012) A portable photoelectric sensor based on colloidal gold immunochromatographic strips for rapid determination of clenbuterol in pig urine. Chin J Anal Chem 40(6):852–856. https://doi.org/10.1016/S1872-2040(11)60553-7 CrossRef

Titel: Development of a nanogold slot blot inhibition assay for the detection of antibodies against bovine herpesvirus type 1
verfasst von: Greice Japolla
Jair Pereira Cunha-Junior
Ana Claudia Arantes Marquez Pajuaba
Ernesto Akio Taketomi
Samira Bührer-Sékula
Luiz Artur Mendes Bataus
Guilherme Rocha Lino de Souza
Publikationsdatum: 21.02.2018
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 6/2018
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-3763-4

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

24.04.2024 | DGIM 2024 | Kongressbericht | Nachrichten

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Development of a nanogold slot blot inhibition assay for the detection of antibodies against bovine herpesvirus type 1

Abstract

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Metformin rückt in den Hintergrund

Myokarditis nach Infekt – richtig schwierig wird es bei Profisportlern

Thrombophiliescreening – Wenn, dann richtig und nur bei therapeutischer Konsequenz

Bei Senioren mit Prostatakarzinom auf Anämie achten!

Update Innere Medizin

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2018

Detection of the antiviral activity of epicatechin isolated from Salacia crassifolia (Celastraceae) against Mayaro virus based on protein C homology modelling and virtual screening

Immune responses of mature chicken bone-marrow-derived dendritic cells infected with Newcastle disease virus strains with differing pathogenicity

Viral shedding and clinical status of feline-norovirus-infected cats after reinfection with the same strain

Molecular evolution and phylodynamics of hepatitis B virus infection circulating in Iran

Myxovirus resistance protein A inhibits hepatitis C virus replication through JAK-STAT pathway activation

Development of a reverse transcription recombinase-aided amplification assay for the detection of coxsackievirus A10 and coxsackievirus A6 RNA

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Metformin rückt in den Hintergrund

Myokarditis nach Infekt – richtig schwierig wird es bei Profisportlern

Thrombophiliescreening – Wenn, dann richtig und nur bei therapeutischer Konsequenz

Bei Senioren mit Prostatakarzinom auf Anämie achten!

Update Innere Medizin