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01.12.2015 | Technical advance | Ausgabe 1/2015 Open Access

BMC Oral Health 1/2015

Development of a point-of-care-device for fast detection of periodontal pathogens

Zeitschrift:
BMC Oral Health > Ausgabe 1/2015
Autoren:
Cornelia Gaertig, Katja Niemann, Jana Berthold, Lisa Giel, Nadine Leitschuh, Christoph Boehm, Liudmila Roussak, Katja Vetter, Dirk Kuhlmeier
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​s12903-015-0155-y) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

CG drafted the manuscript, carried out lyophilization experiments and PCR-on-a-chip together with KN. LG contributed to Southern Blot analysis and JB, NL, LR have been involved in real-time PCR experiments. CB helped with the device’s assembly and developed the software for PCR analysis. KV and DK conceived the project, participated in the design and coordination. All authors read and approved the final manuscript.

Abstract

Background

A number of pathogens can cause severe destruction of the periodontal apparatus during the course of periodontitis. The aim of this work was the development of a diagnostic device for the use at the point-of-need for the detection of periodontal pathogens to enable a personalized therapy for treatment of periodontitis.

Methods

This test system is based on the polymerase chain reaction of DNA isolated from periodontal pathogens and was examined to precisely detect species-specific sequences on a rotating chip with lyophilized reagents for polymerase chain reaction. The preservation of the reagents was optimized to ensure their stability during the storage.

Results

In the current work, we have developed a model point-of-care device and showed a proof of concept. It requires low sample volume, is timesaving and can therefore facilitate early diagnosis and treatment of periodontal diseases.

Conclusions

The developed device can provide fast diagnosis of the composition and amount of patients’ oral flora and might help to assess the stage of periodontitis infection. This can facilitate an optimization of therapeutic approaches in order to prevent some of the more serious consequences of the disease.
Zusatzmaterial
Additional file 1: Table S1. Organisms and cultivation conditions. (XLSX 11 kb)
12903_2015_155_MOESM1_ESM.xlsx
Additional file 2: Table S2. Primer sequences and product size. (XLSX 12 kb)
12903_2015_155_MOESM2_ESM.xlsx
Literatur
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