Electronic supplementary material
Chemicals and reagents
Real-time assessment of HER2 signaling network activity
Data analysis and statistics
For determining the magnitude of the stimulus, CF-C was used.
For determining the absolute amount of HER2 involvement in a particular stimulus in the CELx HSF test, (CF-C)-(CDF-C) was used, combining the EGF and NRG1b stimulus data to arrive at a comparative total amount of HER2 signaling response for a particular cell sample.
Percentage of stimulus signal reduction by drug inhibition was calculated by [1-[(CF-C)-(CDF-C)]/ (CF-C)]*100.