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Erschienen in: Forensic Toxicology 1/2021

10.09.2020 | Original Article

Development of the “selective concentration” analytical method for drug-containing hair regions based on micro-segmental analysis to identify a trace amount of drug in hair: hair analysis following single-dose ingestion of midazolam

verfasst von: Kenji Kuwayama, Hajime Miyaguchi, Tatsuyuki Kanamori, Kenji Tsujikawa, Tadashi Yamamuro, Hiroki Segawa, Yuki Okada, Yuko T. Iwata

Erschienen in: Forensic Toxicology | Ausgabe 1/2021

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Abstract

Purpose

Hair can be the only available specimen to prove drug-facilitated crimes (DFCs). However, it is difficult to identify drugs abused in DFCs, particularly benzodiazepines ingested in a single dose, using the conventional segmental hair analysis. In this study, an analytical procedure to identify a trace amount of drug in hair based on micro-segmental analysis was developed.

Methods

Hair was donated from a subject who had been administered midazolam in a single dose. First, tens of hair strands were analyzed according to our routine procedure. Drug screening and identification were performed using a quadrupole-Orbitrap liquid chromatography–tandem mass spectrometry (LC–MS/MS) instrument. Next, two hair strands were segmented at 0.4-mm intervals and multi-target selected reaction monitoring of each segment (micro-segmental analysis) was performed using a tandem quadrupole LC–MS/MS instrument. After distribution curves of midazolam-derived ions in each hair strand were constructed, the residual extracts from segments corresponding to drug-containing regions were collected selectively into one tube. The extract was concentrated using solid-phase extraction and then injected into the LC–MS/MS instrument for drug identification.

Results

We failed to identify drugs using our routine procedure, although a slight peak, predicted to arise from midazolam, was detected, while the peak detected in the concentrated extract from drug-containing hair regions (named “selective concentration”) was identified as midazolam.

Conclusions

The “selective concentration” enabled the identification of a trace amount of drug using several hair strands. The method would be helpful in proving DFCs even after it was impossible to identify drugs using conventional segmental hair analysis.
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Literatur
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Zurück zum Zitat Kintz P, Salomone A, Vincenti M (2015) Hair analysis in clinical and forensic toxicology. Academic Press, Cambridge Kintz P, Salomone A, Vincenti M (2015) Hair analysis in clinical and forensic toxicology. Academic Press, Cambridge
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Zurück zum Zitat World Anti-Doping Agency Laboratory Expert Group (2015) WADA technical document—TD2015IDCR: minimum criteria for chromatographic-mass spectrometric confirmation of the identity of analytes for doping control purposes. WADA, Montreal World Anti-Doping Agency Laboratory Expert Group (2015) WADA technical document—TD2015IDCR: minimum criteria for chromatographic-mass spectrometric confirmation of the identity of analytes for doping control purposes. WADA, Montreal
Metadaten
Titel
Development of the “selective concentration” analytical method for drug-containing hair regions based on micro-segmental analysis to identify a trace amount of drug in hair: hair analysis following single-dose ingestion of midazolam
verfasst von
Kenji Kuwayama
Hajime Miyaguchi
Tatsuyuki Kanamori
Kenji Tsujikawa
Tadashi Yamamuro
Hiroki Segawa
Yuki Okada
Yuko T. Iwata
Publikationsdatum
10.09.2020
Verlag
Springer Singapore
Erschienen in
Forensic Toxicology / Ausgabe 1/2021
Print ISSN: 1860-8965
Elektronische ISSN: 1860-8973
DOI
https://doi.org/10.1007/s11419-020-00553-8

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