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Erschienen in: Journal of Clinical Immunology 6/2011

01.12.2011

Developmental, Malignancy-Related, and Cross-Species Analysis of Eosinophil, Mast Cell, and Basophil Siglec-8 Expression

verfasst von: Sherry A. Hudson, Harald Herrmann, Jian Du, Paul Cox, El-Bdaoui Haddad, Barbara Butler, Paul R. Crocker, Steven J. Ackerman, Peter Valent, Bruce S. Bochner

Erschienen in: Journal of Clinical Immunology | Ausgabe 6/2011

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Abstract

Objective

The aim of this study is to determine when during hematopoiesis Siglec-8 gets expressed, whether it is expressed on hematologic malignancies, and if there are other non-human species that express Siglec-8.

Methods

Siglec-8 mRNA and cell surface expression was monitored during in vitro maturation of human eosinophils and mast cells. Flow cytometry was performed on human blood and bone marrow samples, and on blood samples from dogs, baboons, and rhesus and cynomolgus monkeys.

Results

Siglec-8 is a late maturation marker. It is detectable on eosinophils and basophils from subjects with chronic eosinophilic leukemia, chronic myelogenous leukemia, and on malignant and non-malignant bone marrow mast cells, as well as the HMC-1.2 cell line. None of the Siglec-8 monoclonal antibodies tested recognized leukocytes from dogs, baboons, and rhesus and cynomolgus monkeys.

Conclusions

Siglec-8-based therapies should not target immature human leukocytes but should recognize mature and malignant eosinophils, mast cells, and basophils. So far, there is no suitable species for preclinical testing of Siglec-8 monoclonal antibodies.
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Metadaten
Titel
Developmental, Malignancy-Related, and Cross-Species Analysis of Eosinophil, Mast Cell, and Basophil Siglec-8 Expression
verfasst von
Sherry A. Hudson
Harald Herrmann
Jian Du
Paul Cox
El-Bdaoui Haddad
Barbara Butler
Paul R. Crocker
Steven J. Ackerman
Peter Valent
Bruce S. Bochner
Publikationsdatum
01.12.2011
Verlag
Springer US
Erschienen in
Journal of Clinical Immunology / Ausgabe 6/2011
Print ISSN: 0271-9142
Elektronische ISSN: 1573-2592
DOI
https://doi.org/10.1007/s10875-011-9589-4

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