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18.01.2016 | General Gynecology | Ausgabe 1/2016

Archives of Gynecology and Obstetrics 1/2016

Diagnosis of aerobic vaginitis by quantitative real-time PCR

Zeitschrift:
Archives of Gynecology and Obstetrics > Ausgabe 1/2016
Autoren:
T. A. Rumyantseva, G. Bellen, Y. A. Savochkina, A. E. Guschin, G. G. G. Donders
Wichtige Hinweise
This work was an award winning presentation at the 1st ISIDOG/9th ESIDOG European conference in Riga, 29th Oct–1st Nov 2015.

Abstract

Purpose

To evaluate a real-time PCR-based technique to quantify bacteria associated with aerobic vaginitis (AV) as a potential test.

Methods

Vaginal samples from 100 women were tested by wet-mount microscopy, gram stain and quantitative real-time PCR targeting Enterobacteriacea, Staphylococcus spp., Streptococcus spp., Enterococcus spp., Escherichia coli, Streptococcus agalactiae, S. aureus; Lactobacillus spp. AV diagnosis obtained by wet-mount microscopy was used as reference.

Results

Some level of AV was diagnosed in 23 (23.7 %) cases. Various concentrations of Enterobacteriacea, Staphylococcus spp., Streptococcus spp. were detected an all patients. Enterococcus spp. were detected in 76 (78.3 %) cases. Summarized concentrations of aerobes were tenfold higher in AV-positive compared to AV-negative cases [7.30lg vs 6.06lg (p = 0.02)]. Concentrations of aerobes in severe, moderate and light AV cases did not vary significantly (p = 0.14). Concentration of lactobacilli was 1000-fold lower in AV-positive cases compared to normal cases (5.3lg vs 8.3lg, p < 0.0001). Streptococcus spp. dominated in the majority of AV-positive cases [19/22 (86.4 %) samples]. The relation of high loads of aerobes to the low numbers of Lactobacilli are a reliable marker for the presence of AV and could substitute microscopy as a test.

Conclusions

PCR may be a good standardized substitution for AV diagnosis in settings where well-trained microscopists are lacking.

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