Background
Disease burden due to persistent digestive disorders in the tropics
Neglected tropical diseases and their contribution to persistent digestive disorders
NIDIAG: developing diagnosis-treatment algorithms for neglected clinical syndromes
Goal, aims and objectives
Aims
Specific objectives and activities
Methods/Design
Study area
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Côte d’Ivoire
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Hôpital Méthodiste de Dabou, a regional reference hospital based in Dabou, approximately 30 km west of Abidjan, the country’s economic capital.
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Indonesia
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Tulehu Hospital, based in Tulehu in Maluku province, approximately 100 km northeast of Ambon City, the province’s capital; and
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Tulehu Health Centre, also situated in Maluku province in close proximity (approximately 1 km) to the Tulehu Hospital.
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Mali
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Niono District Reference Health Centre, based in Niono, approximately 300 km northeast of the country’s capital Bamako; and
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Institut National de Recherche en Santé Publique (INRSP) in Bamako.
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Nepal
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B P Koirala Institute of Health Sciences, situated in Dharan, approximately 350 km southeast of the country’s capital Kathmandu; and
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Dhankuta District Hospital in Dhankuta, approximately 50 km north of Dharan.
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Study design
Study participants
Sample size
Patient recruitment and clinical management
Diagnostic tests and laboratory procedures
Diagnostic test | Targeted pathogens | Reference(s) |
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Microscopy | ||
Direct faecal smear | Helminths, intestinal protozoa | [55] |
Kato-Katz thick smear | Helminths | [56] |
Acid-fast staining | Cryptosporidium spp., Cyclospora cayetanensis, Cystoisospora belli | [57] |
Baermann funnel concentration technique | Strongyloides stercoralis, hookworm | |
Formalin-ether concentration technique | Helminths, intestinal protozoa | |
Mini-FLOTAC | Helminths | |
Culture | ||
Bacteriological stool culture | Salmonella spp., Shigella spp., Campylobacter spp., Yersinia spp. | [9] |
Koga agar culture | Strongyloides stercoralis, hookworm | |
Rapid diagnostic tests (RDTs) | ||
Crypto/Giardia DuoStrip | Cryptosporidium spp., Giardia intestinalis | [37] |
Circulating cathodic antigen (CCA)a |
Schistosoma mansoni
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Molecular post-hoc testing on ethanol-fixed stool samples | ||
Multiplex PCR | Helminths, intestinal protozoa (all samples); diarrhoeagenic bacteria and viruses (selected sub-sample) |