Diagnostic and treatment modalities for patients with cervical lymph node metastases of unknown primary site – current status and challenges

If the patient history reflects excessive use of alcohol and tobacco, the primary site is unlikely to be situated in the nasopharynx, whereas promiscuity and orogenital contact suggest findings within the oropharynx. Also a history of skin lesions of the head and neck can guide the search [44]. The patient usually presents with a painless, unilateral cervical mass. Affliction of the levels 1–3 indicates the primary site to be located in the head and neck region, whereas a mass in levels 4–5 suggests the primary tumor situated at the lower neck (e.g., thyroid gland) or below the clavicles [31‐33] (Fig. 1). Further examination is performed through exploring the head and upper aerodigestive tract using a nasopharyngoscope.

FNAB (Fine-needle aspiration biopsy) of the cervical mass is the first and most commonly used diagnostic procedure, as it is minimal invasive and associated with a negligible risk of spreading the tumor along the needle’s path. After routine staining, the diagnostic sensitivity for metastatic neck lymph nodes ranges from 83 to 97% with a specifity of 91–100% when performed by an experienced histopathologist [45].

Immunohistochemistry (IHC) is an important tool to identify the tissue’s origin. General staining identifies cell morphologies and abnormal/malignant cell populations. Afterwards, an initial IHC-panel for broad cancer types including epithelial, melanocytic and lymphoid markers is used. General markers for carcinomas are the cytokeratins, i.e., intermediate filaments specific to epithelium. Markers for lymphomas are CLA – common leukocyte antigen, ALK1 – anaplastic lymphoma kinase, CD30 and CD43. For melanomas there are S-100, HMB45 – anti-human melanosome, and Melan-A [46]. In case of carcinoma, its subtype is evaluated by considering morphological aspects followed by specific antibodies, such as CK5, CK6, CK7 or TTF-1 [46]. The most common tumor types for HNCUP are squamous cell carcinomas SCC and adenocarcinomas.

Available imaging techniques for patients with HNCUP are CT- and MRI- as well as 18F-fluoro2-deoxyglucose positron emission tomography (FDG-PET)-scans (Fig. 1). A quick, inexpensive, procedure with high spatial resolution is the contrast-enhanced CT-scan from the skull base to clavicles, complemented or substituted by a gadolinium contrast-enhanced MRI with superior soft tissue resolution [43]. In case of a cervical lymph node metastasis, the chance for CT, MRI or both to detect the primary site ranges from 9 to 23% [7, 47‐49]. When suspicious findings on imaging are used to guide biopsy, the chance to find the primary tumor rises up to 60% [50]. For lymph nodes located in levels 4–5, additional chest/abdominal/pelvic CT-scans are recommended [51]. FDG-PET is a useful diagnostic tool when standard radiological work-up is completed with negative or inconclusive results and should be performed before any invasive procedures, which possibly hamper the evaluation of the scans due to iatrogenic induced tissue alteration [52‐56]. Its capability for tumor detection is down to a size of ≥5 mm. Several studies and reviews adressed the additional benefits of FDG-PET for patients with HNCUP (Table 1) [54‐56]. The extent of pre-FDG-PET diagnostic workup differs between the studies, so that it becomes difficult to compare the reported sensitivities and specifities and to quantify the additional value of PET [57].

Panendoscopy of the upper aerodigestive tract (P-UADT), including naso-, oro- and hypopharynx as well as laryngoscopy and esophagoscopy, is performed under general anesthesia. Biopsies are taken from radiologically or clinically suspicious sites [43]. Additional bronchoscopy is recommended when indicated by imaging [44]. Repetition of panendoscopy is only indicated when directed biospy failed during the first procedure [50, 58]. Ipsilateral tonsillectomy leads to primary tumor detection in 18–44.6% of the cases. Waltonen et al. [47] reported the highest success rate for detection of the primary tumor by PET-CT scans plus panendoscopy with directed biopsies, with or without tonsillectomy (59.6%).

HPV DNA, when found in metastasis, directs the search for the primary tumor to the oropharynx, as the prevalence of HPV in non-oropharyngeal squamous cell cancers currently is only 22%. HPV status can be determined by in-situ hybridization (ISH) or polymerase chain reaction (PCR), dectecting HPV DNA or by HPV E6/E7 RNA expression detected by quantitative reverse transcriptase-PCR (qRT-PCR). As a HPV surrogate marker, immunohistochemical staining of p16, a human tumor-suppressor protein [59‐65], is also widely used. Despite showing a significantly improved disease-free survival, some authors like Dixon et al. could not find an improved overall survival for p16-positive HNCUP patients in their studies [66]. Other reports showed a significant positive impact of HPV/p16 only when combined with other factors like (non-)smoking [67]. A meta-analysis published in 2007 regarding non-oropharyngeal HNSCC shows congruent results [68]. However, most of the published literature agrees that HPV/p16 is a positive prognostic indicator for HNCUP [69, 70].

TP53 (protein name: p53) is a tumor-suppressor gene which is altered in about 50% of human malignancies, either by mutation or inactivation due to viral or cellular protein interactions leading to p53 degradation [71]. Significantly impaired outcome for patients with mutated p53 status or overexpression of p53 (whose expression directly correlates with the mutated protein, as the second tends to accumulate) in HNCUP and HNSCC has been demonstrated before [72, 73]. Some of the authors also examined the impact of the combined HPV/p53 status on survival and came to the conclusion that p53 could be an independent prognostic factor regardless of the HPV status [74].

Epstein-Barr virus (EBV) is consistently associated with nasopharyngeal carcinoma (NPC), especially with poorly or undifferentiated and nonkeratinizing types [75]. NPC is much more common in southern China and southeast Asia than in Europe or north America [74]. EBVs latent membrane protein 1 is highly suspicious of having a central role in both initiation and progression of the tumor [76‐78]. EBV-DNA is routinely detected by PCR with sensitivity and specifity close to 90% from FNAB samples [79‐82].

The data above suggest that the importance of detecting the HPV and EBV-DNA (or their surrogate proteins) in a metastatic lymph node in CUP-disease is high, as it can guide both further diagnostics and treatment (e.g., new directed biopsies or a radiotherapy-boost directed to the assumed primary tumor site) and also predict the patient’s outcome. These assays should be implemented in clinical routine for every HNCUP case. Immunohistochemistry for p53 is a simple and inexpensive method for further prognostic stratification and could be used as an additional prognostic parameter.

Early-stage HNCUP is defined as pN1 or mobile pN2a without extracapsular extention (ECE). Adequate regional control was reported both by studies performing surgery or radiotherapy (RT) as monotherapy. A bias exists, since usually patients with greater neck burden are treated more likely with RT primarily [10]. Although policy-dependend approaches prefer surgery alone with the option of salvage-RT [16, 84] or vice versa [15], there is some evidence for primary surgery: only pathology after surgery reliably proves ECE, which then necessitates RT with concomitant chemotherapy (CTx) and the vast majority of the patients in the published series implemented this approach (Table 2). In pN1 or pN2a situations without ECE, postoperative RT has not proven clear benefit regarding locoregional control or survival [85, 86]. However, some of the few studies addressing this topic are biased, due to their retrospective nature and the simplified statistics used [87, 88], so that the role of postoperative RT in these situations remains unclear. However, when RT was postponed and used for salvage treatment only, ultimate control above the clavicles still reached more than 90% in pN1 situations without ECE [84]. Surgery should also be followed by adjuvant RT in cases of connective tissue invasion (ECE), more than one involved node and a likelihood of residual microscopic disease in the neck (R1) [87, 88]. In cases without these risk factors postoperative RT could be considered.

In advanced-stage neck disease (N2b-N3) a multimodal approach consisting of surgery and RT with or witout CTx is most common and seems to provide superior results regarding survival when compared to single-modality treatment [15, 18, 23]. This is true for the combination of surgery and RT compared to RT alone [25, 27, 89], as well as for surgery and RT compared to surgery alone, at least regarding the subsequent emergence of a primary tumor [15]. In cases of an unresectable bulk or unambiguously anticipated ECE/incomplete resection, primary chemoradiotherapy (CRT) is the treatment of choice in order to avoid excess toxicity from surgery and postoperative chemoradiotherapy. In most of the retrospective studies above, early-stage disease (1 small node involved) was surgically treated and unresectable masses primary irradiated, which could have biased the results and makes data interpretation difficult. However, due to the lack of prospective data, many crucial questions regarding the optimal radiotherapeutical treatment remain: uni- versus bilateral neck treatment, mucosal irradiation and the use of concomitant chemotherapy.

In 2001, Nieder et al. [90] reviewed the management of HNCUP and reported results of various groups regarding ipsilateral versus mucosal and bilateral irradiation. Some results showed decreased tumor control and survival for ipsilateral therapy, while others failed to show any significant differences in outcomes between sole ipsilateral RT and comprehensive treatment of both neck sides and mucosa. When disease control was examined, there was no evidence supporting extended volume treatment over ipsilateral RT. The authors recommended a randomized trial between both options, but a similar trial was never accomplished: a prospective randomized trial (EORTC-24001-22005) starting in 2002 to compare ipsilateral versus bilateral plus mucosal irradiation in HNCUP failed to provide any results, due to very limited patient enrollment. Table 2 demonstrates that most of the larger studies included unilateral as well as bilateral treatment in varying proportions. However, no obvious outcome differences exist between those that treated predominantely unilateral (e.g., Straetmans et al., Patel et al.) and those who preffered a unilateral irradiation (e.g., Wallace et al., Fakhrian et al.), at least regarding overall survival. A recent large meta-analysis revealed no significant differences in 5-year-overall- and disease free survival (OS and DFS) between ipsi- and bilateral RT, but improved locoregional control and lower recurrence rates in favor of bilateral treatment [89]. When considering additional mucosal treatment (“presumed primary tumor”), recurrence rates were significantly lower and DFS better when extended radiation volumes where used, but no benefit for OS could be found and the improved locoregional control was associated with significantly higher severe toxicity [89].

In the current NCCN guidelines [91] no clear statement about the treatment volumes is being made, the approaches found in the literature vary and many of these data originate from the time before the routine use of PET and tonsillectomy. As diagnostic workup became more comprehensive, it could be shown that the numbers of patients developing primary site tumor is lower than indicated in previous literature [15] and also about twofold lower than the risk for nodal recurrence or distant metastases [90]. It seems that metastatic disease in general is nowadays the most common pattern of failure [92, 93], so that a possible benefit of a slightly improved locoregional control through extended volume radiotherapy can not be translated in an improved survival [84].

The advent of intensity modulated radiotherapy (IMRT) made the more sophisticated selection of the irradiation volumes essential, as it allows both sparing of organs at risk as well as missing the primary that would had been accidentally treated using older techniques. Previously, HNSCC/HNCUP was treated using a three-field technique including all mucosal sites and both sites of the neck [94], whereas today’s standard is intensity- IMRT preserving salivatory tissues [95]. The vast majority of the data presented here (Table 2) have been generated with older, non-conformal techniques. However, a possible strategy in modern series treating HNCUP could be the irradiation of selected mucosal sites, e.g., base of tongue for HPV-positive non-smokers or nasopharynx for EBV-positive non-smokers with nonkeratinizing subtypes and/or patients with Asiatic origin. Such approaches have become more common in the IMRT-era and the first data are encouraging [84, 96].

An overview of the radiation doses and treatment volumes in the greatest series published can be found in Table 2.

The value of adding chemotherapy to RT both in the definitive as in the postoperative setting for treating HNCUP patients remains unclear, despite its common use in many institutions [27, 29, 67, 97, 98]. Cisplatin (e.g., 100 mg/m², days 1, 22 and 43) is the agent most frequently used in these cases [24, 99]. Established indications for concomitant chemotherapy in HNSCC are the definitive treatment of locally advanced tumors (e.g., a cT2cN2b tumor) or the postoperative treatment of high-risk tumors (e.g., a pT1pN3b tumor: extracapsular extension). Implementing chemotherapy for a HNCUP with one or more involved nodes after neck dissection would assume that it has a similar prognosis with such cases. This does not seem justified, since a cT1 tumor (in this case not detected, therefore CUP) generally has an excellent prognosis with RT alone [84, 100]. A recent study by Hosni et al. revealed an almost identical prognosis for patients with HNCUP and those with T1 base-of-tongue carcinoma [101]. These data would imply that both diseases may be treated the same way, i.e., without the use of chemotherapy. In a retrospective analysis examining the effect of concomitant cisplatin, involving 60 HNCUP-patients, no clear advantage could be found for the addition of chemotherapy and severe toxicities (grade 3+) occurred significantly more often [24]. Furthermore, in the era of HPV/p16 stratification a de-escalation of treatment and an alternative staging for positive tumors are already under discussion because of the distinct improved outcomes of this collective [102, 103]. The current paradigm for the indications for postoperative chemo-irradiation (R1, pN3b) originates from the pre-HPV-stratification era [104, 105]. Keller et al. [70] have conducted an analysis of clinicopathological data, including p16 and extracapsular extension (ECE), in HNCUP and could demonstrate a very similar prognosis in patients with or without ECE, even without chemotherapy, but the patient numbers in this analysis where very limited and so no safe conclusions can be drawn. A treatment-deescalation for HPV/p16 non-smokers could be imaginable, either through omitting chemotherapy or even by using chemotherapy in order to reduce RT-dose, following the paradigm of current HNSCC trials [102]. Table 3 shows the largest published studies implementing chemotherapy and the agents used in each case [10, 11, 53, 76, 77, 79‐85].