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Erschienen in:

26.08.2020 | Original Article

Diagnostic criteria of sentinel lymph node micrometastasis or macrometastasis based on tissue rinse liquid-based cytology in gynecological cancer

verfasst von: Hiroko Matsumiya, Yukiharu Todo, Hiroyuki Yamazaki, Ryutaro Yamada, Kaoru Minowa, Tomohiko Tsuruta, Hiroyuki Kurosu, Shinichiro Minobe, Hidenori Kato, Hiroaki Suzuki, Kiyomi Taira, Katsushige Yamashiro

Erschienen in: International Journal of Clinical Oncology | Ausgabe 12/2020

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Abstract

Objective

This study aimed to establish intraoperative diagnostic criteria of sentinel lymph node (SLN) micro-/macrometastasis on the basis of tissue rinse liquid-based cytology (TRLBC) in gynecological cancer.

Methods

We enrolled 214 patients with gynecological cancer who underwent rapid diagnosis of SLN metastasis on the basis of TRLBC from a total of 490 SLNs. For slides that were classified as positive for atypical cells on cytological inspection, we counted the number of clusters (an atypical cell mass consisted of three or more cells) and the number of single cells (an atypical cell other than clusters). Receiver operating characteristic (ROC) analysis was applied to determine the efficiency of predicting SLN micro-/macrometastasis.

Results

On cytological inspection, 36 slides were classified as positive for atypical cells, while 21 slides (4.3%) were true positive, 15 (3.1%) were false positive, and 454 (92.6%) were true negative. There were no false negative results in this study. The area under the ROC curve for the number of cluster was superior to that for the number of single cells for distinguishing micro-/macrometastasis from negative/isolated tumor cells (0.86 vs. 0.67, P = 0.032). The optimum cut-off value of the number of clusters was 5 for distinguishing these two categories.

Conclusions

TRLBC is a highly sensitive alternative for detecting SLN metastasis as a rapid intraoperative diagnosis. Counting the number of atypical cell clusters might be useful for distinguishing micro-/macrometastasis from isolated tumor cells.
Literatur
1.
Zurück zum Zitat Niikura H, Tsuji K, Tokunaga H et al (2019) Sentinel node navigation surgery in cervical and endometrial cancer: a review. Jpn J Clin Oncol 49:495–500CrossRef Niikura H, Tsuji K, Tokunaga H et al (2019) Sentinel node navigation surgery in cervical and endometrial cancer: a review. Jpn J Clin Oncol 49:495–500CrossRef
2.
Zurück zum Zitat Kadkhodayan S, Hasanzadeh M, Treglia G et al (2015) Sentinel node biopsy for lymph nodal staging of uterine cervix cancer: a systematic review and meta-analysis of the pertinent literature. Eur J Surg Oncol 41:1–20CrossRef Kadkhodayan S, Hasanzadeh M, Treglia G et al (2015) Sentinel node biopsy for lymph nodal staging of uterine cervix cancer: a systematic review and meta-analysis of the pertinent literature. Eur J Surg Oncol 41:1–20CrossRef
3.
Zurück zum Zitat Altgassen C, Hertel H, Brandstädt A et al (2008) Multicenter validation study of the sentinel lymph node concept in cervical cancer: AGO Study Group. J Clin Oncol 26:2943–2951CrossRef Altgassen C, Hertel H, Brandstädt A et al (2008) Multicenter validation study of the sentinel lymph node concept in cervical cancer: AGO Study Group. J Clin Oncol 26:2943–2951CrossRef
4.
Zurück zum Zitat Lécuru F, Mathevet P, Querleu D et al (2011) Bilateral negative sentinel nodes accurately predict absence of lymph node metastasis in early cervical cancer: results of the SENTICOL study. J Clin Oncol 29:1686–1691CrossRef Lécuru F, Mathevet P, Querleu D et al (2011) Bilateral negative sentinel nodes accurately predict absence of lymph node metastasis in early cervical cancer: results of the SENTICOL study. J Clin Oncol 29:1686–1691CrossRef
5.
Zurück zum Zitat Ballester M, Dubernard G, Lécuru F et al (2011) Detection rate and diagnostic accuracy of sentinel-node biopsy in early stage endometrial cancer: a prospective multicentre study (SENTI-ENDO). Lancet Oncol 12:469–476CrossRef Ballester M, Dubernard G, Lécuru F et al (2011) Detection rate and diagnostic accuracy of sentinel-node biopsy in early stage endometrial cancer: a prospective multicentre study (SENTI-ENDO). Lancet Oncol 12:469–476CrossRef
6.
Zurück zum Zitat Rossi EC, Kowalski LD, Scalici J et al (2017) A comparison of sentinel lymph node biopsy to lymphadenectomy for endometrial cancer staging (FIRES trial): a multicentre, prospective, cohort study. Lancet Oncol 18:384–392CrossRef Rossi EC, Kowalski LD, Scalici J et al (2017) A comparison of sentinel lymph node biopsy to lymphadenectomy for endometrial cancer staging (FIRES trial): a multicentre, prospective, cohort study. Lancet Oncol 18:384–392CrossRef
7.
Zurück zum Zitat Frumovitz M, Plante M, Lee PS et al (2018) Near-infrared fluorescence for detection of sentinel lymph nodes in women with cervical and uterine cancers (FILM): a randomised, phase 3, multicentre, non-inferiority trial. Lancet Oncol 19:1394–1403CrossRef Frumovitz M, Plante M, Lee PS et al (2018) Near-infrared fluorescence for detection of sentinel lymph nodes in women with cervical and uterine cancers (FILM): a randomised, phase 3, multicentre, non-inferiority trial. Lancet Oncol 19:1394–1403CrossRef
8.
Zurück zum Zitat Van Diest PJ, Torrenga H, Borgstein PJ et al (1999) Reliability of intraoperative frozen section and imprint cytological investigation of sentinel lymph nodes in breast cancer. Histopathology 35:14–18CrossRef Van Diest PJ, Torrenga H, Borgstein PJ et al (1999) Reliability of intraoperative frozen section and imprint cytological investigation of sentinel lymph nodes in breast cancer. Histopathology 35:14–18CrossRef
9.
Zurück zum Zitat Veronesi U, Paganelli G, Galimberti V et al (1997) Sentinel-node biopsy to avoid axillary dissection in breast cancer with clinically negative lymph-nodes. Lancet 349:1864–1867CrossRef Veronesi U, Paganelli G, Galimberti V et al (1997) Sentinel-node biopsy to avoid axillary dissection in breast cancer with clinically negative lymph-nodes. Lancet 349:1864–1867CrossRef
10.
Zurück zum Zitat Motomura K, Inaji H, Komoike Y et al (2000) Intraoperative sentinel lymph node examination by imprint cytology and frozen sectioning during breast surgery. Br J Surg 87:597–601CrossRef Motomura K, Inaji H, Komoike Y et al (2000) Intraoperative sentinel lymph node examination by imprint cytology and frozen sectioning during breast surgery. Br J Surg 87:597–601CrossRef
11.
Zurück zum Zitat Zgajnar J, Frkovic-Grazio S, Besic N et al (2004) Low sensitivity of the touch imprint cytology of the sentinel lymph node in breast cancer patients–results of a large series. J Surg Oncol 85:82–86CrossRef Zgajnar J, Frkovic-Grazio S, Besic N et al (2004) Low sensitivity of the touch imprint cytology of the sentinel lymph node in breast cancer patients–results of a large series. J Surg Oncol 85:82–86CrossRef
12.
Zurück zum Zitat Contractor K, Gohel M, Al-Salami E et al (2009) Intra-operative imprint cytology for assessing the sentinel node in breast cancer: results of its routine use over 8 years. Eur J Surg Oncol 35:16–20CrossRef Contractor K, Gohel M, Al-Salami E et al (2009) Intra-operative imprint cytology for assessing the sentinel node in breast cancer: results of its routine use over 8 years. Eur J Surg Oncol 35:16–20CrossRef
13.
Zurück zum Zitat Pugliese MS, Kohr JR, Allison KH et al (2006) Accuracy of intraoperative imprint cytology of sentinel lymph nodes in breast cancer. Am J Surg 192:516–519CrossRef Pugliese MS, Kohr JR, Allison KH et al (2006) Accuracy of intraoperative imprint cytology of sentinel lymph nodes in breast cancer. Am J Surg 192:516–519CrossRef
14.
Zurück zum Zitat Weiser MR, Montgomery LL, Susnik B et al (2000) Is routine intraoperative frozen-section examination of sentinel lymph nodes in breast cancer worthwhile? Ann Surg Oncol 7:651–655CrossRef Weiser MR, Montgomery LL, Susnik B et al (2000) Is routine intraoperative frozen-section examination of sentinel lymph nodes in breast cancer worthwhile? Ann Surg Oncol 7:651–655CrossRef
15.
Zurück zum Zitat Tsujimoto M, Nakabayashi K, Yoshidome K et al (2007) One-step nucleic acid amplification for intraoperative detection of lymph node metastasis in breast cancer patients. Clin Cancer Res 13:4807–4816CrossRef Tsujimoto M, Nakabayashi K, Yoshidome K et al (2007) One-step nucleic acid amplification for intraoperative detection of lymph node metastasis in breast cancer patients. Clin Cancer Res 13:4807–4816CrossRef
16.
Zurück zum Zitat Tamaki Y, Akiyama F, Iwase T et al (2009) Molecular detection of lymph node metastases in breast cancer patients: results of a multicenter trial using the one-step nucleic acid amplification assay. Clin Cancer Res 15:2879–2884CrossRef Tamaki Y, Akiyama F, Iwase T et al (2009) Molecular detection of lymph node metastases in breast cancer patients: results of a multicenter trial using the one-step nucleic acid amplification assay. Clin Cancer Res 15:2879–2884CrossRef
17.
Zurück zum Zitat Tamaki Y (2017) One-step nucleic acid amplification (OSNA): where do we go with it? Int J Clin Oncol 22:3–10CrossRef Tamaki Y (2017) One-step nucleic acid amplification (OSNA): where do we go with it? Int J Clin Oncol 22:3–10CrossRef
18.
Zurück zum Zitat Tamaki Y, Sato N, Homma K et al (2012) Routine clinical use of the one-step nucleic acid amplification assay for detection of sentinel lymph node metastases in breast cancer patients: results of a multicenter study in Japan. Cancer 118:3477–3483CrossRef Tamaki Y, Sato N, Homma K et al (2012) Routine clinical use of the one-step nucleic acid amplification assay for detection of sentinel lymph node metastases in breast cancer patients: results of a multicenter study in Japan. Cancer 118:3477–3483CrossRef
19.
Zurück zum Zitat Rossi ED, Mulè A, Russo RM et al (2008) Application of liquidbased preparation to non-gynaecologic exfoliative cytology. Pathologica 100:461–465PubMed Rossi ED, Mulè A, Russo RM et al (2008) Application of liquidbased preparation to non-gynaecologic exfoliative cytology. Pathologica 100:461–465PubMed
20.
Zurück zum Zitat Laucirica R, Bentz JS, Souers RJ et al (2010) Do liquid-based preparations of urinary cytology perform differently than classically prepared cases? Observations from the College of American Pathologists Interlaboratory Comparison Program in Nongynecologic Cytology. Arch Pathol Lab Med 134:19–22PubMed Laucirica R, Bentz JS, Souers RJ et al (2010) Do liquid-based preparations of urinary cytology perform differently than classically prepared cases? Observations from the College of American Pathologists Interlaboratory Comparison Program in Nongynecologic Cytology. Arch Pathol Lab Med 134:19–22PubMed
21.
Zurück zum Zitat Yamashiro K, Taira K, Nakajima M et al (2012) Tissue rinse liquid-based cytology: a feasible tool for the intraoperative pathological evaluation of sentinel lymph nodes in breast cancer patients. Cytopathology 23:263–269CrossRef Yamashiro K, Taira K, Nakajima M et al (2012) Tissue rinse liquid-based cytology: a feasible tool for the intraoperative pathological evaluation of sentinel lymph nodes in breast cancer patients. Cytopathology 23:263–269CrossRef
23.
Zurück zum Zitat Esselen KM, Terry KL, Samuel A et al (2016) Endosalpingiosis: more than just an incidental finding at the time of gynecologic surgery? Gynecol Oncol 142:255–260CrossRef Esselen KM, Terry KL, Samuel A et al (2016) Endosalpingiosis: more than just an incidental finding at the time of gynecologic surgery? Gynecol Oncol 142:255–260CrossRef
24.
Zurück zum Zitat Prentice L, Stewart A, Mohiuddin S et al (2012) What is endosalpingiosis? Fertil Steril 98:942–947CrossRef Prentice L, Stewart A, Mohiuddin S et al (2012) What is endosalpingiosis? Fertil Steril 98:942–947CrossRef
25.
Zurück zum Zitat Salehi AH, Omeroglu G, Kanber Y et al (2013) Endosalpingiosis in axillary lymph nodes simulating metastatic breast carcinoma: a potential diagnostic pitfall. Int J Surg Pathol 21:610–612CrossRef Salehi AH, Omeroglu G, Kanber Y et al (2013) Endosalpingiosis in axillary lymph nodes simulating metastatic breast carcinoma: a potential diagnostic pitfall. Int J Surg Pathol 21:610–612CrossRef
26.
Zurück zum Zitat Shiino S, Yoshida M, Jimbo K et al (2019) Two rare cases of endosalpingiosis in the axillary sentinel lymph nodes: evaluation of immunohistochemical staining and one-step nucleic acid amplification (OSNA) assay in patients with breast cancer. Virchows Arch 474:633–638CrossRef Shiino S, Yoshida M, Jimbo K et al (2019) Two rare cases of endosalpingiosis in the axillary sentinel lymph nodes: evaluation of immunohistochemical staining and one-step nucleic acid amplification (OSNA) assay in patients with breast cancer. Virchows Arch 474:633–638CrossRef
Metadaten
Titel
Diagnostic criteria of sentinel lymph node micrometastasis or macrometastasis based on tissue rinse liquid-based cytology in gynecological cancer
verfasst von
Hiroko Matsumiya
Yukiharu Todo
Hiroyuki Yamazaki
Ryutaro Yamada
Kaoru Minowa
Tomohiko Tsuruta
Hiroyuki Kurosu
Shinichiro Minobe
Hidenori Kato
Hiroaki Suzuki
Kiyomi Taira
Katsushige Yamashiro
Publikationsdatum
26.08.2020
Verlag
Springer Singapore
Erschienen in
International Journal of Clinical Oncology / Ausgabe 12/2020
Print ISSN: 1341-9625
Elektronische ISSN: 1437-7772
DOI
https://doi.org/10.1007/s10147-020-01774-y

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