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Erschienen in: The journal of nutrition, health & aging 8/2019

30.08.2019

Diet Modulation Restores Autophagic Flux in Damaged Skeletal Muscle Cells

verfasst von: F. M. Giordano, S. Burattini, F. Buontempo, B. Canonico, A. M. Martelli, S. Papa, M. Sampaolesi, E. Falcieri, Sara Salucci

Erschienen in: The journal of nutrition, health & aging | Ausgabe 8/2019

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Abstract

Objectives

Autophagy is a physiological and highly regulated mechanism, crucial for cell homeostasis maintenance. Its impairment seems to be involved in the onset of several diseases, including muscular dystrophies, myopathies and sarcopenia. According to few papers, chemotherapeutic drug treatment is able to trigger side effects on skeletal muscle tissue and, among these, a defective autophagic activation, which leads to the persistence of abnormal organelles within cells and, finally, to myofiber degeneration. The aim of this work is to find a strategy, based on diet modulation, to prevent etoposide-induced damage, in a model of in vitro skeletal muscle cells.

Methods

Glutamine supplementation and nutrient deprivation have been chosen as pre-treatments to counteract etoposide effect, a chemotherapeutic drug known to induce oxidative stress and cell death. Cell response has been evaluated by means of morpho-functional, cytofluorimetric and molecular analyses.

Results

Etoposide treated cells, if compared to control, showed dysfunctional mitochondria presence, ER stress and lysosomal compartment damage, confirmed by molecular investigations.

Conclusions

Interestingly, both dietary approaches were able to rescue myofiber from etoposide-induced damage. Glutamine supplementation, in particular, seemed to be a good strategy to preserve cell ultrastructure and functionality, by preventing the autophagic impairment and partially restoring the normal lysosomal activity, thus maintaining skeletal muscle homeostasis.
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Metadaten
Titel
Diet Modulation Restores Autophagic Flux in Damaged Skeletal Muscle Cells
verfasst von
F. M. Giordano
S. Burattini
F. Buontempo
B. Canonico
A. M. Martelli
S. Papa
M. Sampaolesi
E. Falcieri
Sara Salucci
Publikationsdatum
30.08.2019
Verlag
Springer Paris
Erschienen in
The journal of nutrition, health & aging / Ausgabe 8/2019
Print ISSN: 1279-7707
Elektronische ISSN: 1760-4788
DOI
https://doi.org/10.1007/s12603-019-1245-3

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