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Erschienen in: The journal of nutrition, health & aging 10/2018

04.08.2018

Dietary Ingestion of Calories and Micronutrients Modulates the DNA Methylation Profile of Leukocytes from Older Individuals

verfasst von: J. Passador, L. V. Toffoli, K. B. Fernandes, R. D. Neves-Souza, Gislaine Garcia Pelosi, M. V. Gomes

Erschienen in: The journal of nutrition, health & aging | Ausgabe 10/2018

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Abstract

Objective

Several lines of evidence from the last decade support the connection between nutrition and epigenetic mechanisms. In the present study we evaluated the impact of the daily dietary intake of calories and the micronutrients vitamin A, D, B1, B2, B5, C, E, copper, calcium, phosphorus, iron, iodine, selenium, manganese, potassium and sodium on the global DNA methylation profile of blood cells from older individuals.

Research Methods & Procedures

The study enrolled 126 physically independent elderly of both sexes (60 men and 66 women). For the molecular analysis, DNA samples were extracted from leukocytes and global DNA methylation was evaluated using a high throughput Elisa-based method. Correlations between global DNA methylation and the daily intake of calorie or micronutrients were evaluated using Prism5 GraphPad Software.

Results

A statistically significant correlation was observed between global DNA methylation and the daily caloric value (p=0.019, r=-0.21), and the intake of vitamin A (p=0.03, r=-0.18), Vitamin E (p=0.027, r=-0.20) and copper (p=0.04, r=-0.18). No correlation was observed between global DNA methylation and the daily intake of vitamin D, B1, B2, B5, C, calcium, phosphorus, iron, iodine, selenium, manganese and potassium (p>0.05).

Conclusion

Our data demonstrate that the daily intake of calories or the micronutrients vitamin A, vitamin E and copper can potentially modulate the global DNA methylation profile of leukocytes in older adults and corroborate the notion of nutritional influences on epigenetic mechanisms.
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Metadaten
Titel
Dietary Ingestion of Calories and Micronutrients Modulates the DNA Methylation Profile of Leukocytes from Older Individuals
verfasst von
J. Passador
L. V. Toffoli
K. B. Fernandes
R. D. Neves-Souza
Gislaine Garcia Pelosi
M. V. Gomes
Publikationsdatum
04.08.2018
Verlag
Springer Paris
Erschienen in
The journal of nutrition, health & aging / Ausgabe 10/2018
Print ISSN: 1279-7707
Elektronische ISSN: 1760-4788
DOI
https://doi.org/10.1007/s12603-018-1085-6

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