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Journal of Gastroenterology

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01.09.2009 | Original Article—Liver, Pancreas, and Biliary Tract

Dietary olive oil prevents carbon tetrachloride-induced hepatic fibrosis in mice

verfasst von: Nobuyuki Tanaka, Hiroshi Kono, Kenichi Ishii, Naohiro Hosomura, Hideki Fujii

Erschienen in: Journal of Gastroenterology | Ausgabe 9/2009

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Abstract

Aim

The specific purpose of this study was to investigate the effects of dietary olive oil on hepatic fibrosis induced by chronic administration of carbon tetrachloride (CCl₄) in the mouse. In addition, the effects of oleic acid, a major component of olive oil, on activation of hepatic stellate cells (HSCs) were investigated in vitro.

Methods

Mice were fed liquid diets containing either corn oil (control, AIN-93) or olive oil (6.25 g/L) throughout experiments. Animals were treated with CCl₄ for 4 weeks intraperitoneally. The mRNA expression of TGF-β1 and collagen 1α2 (col1α2) in the liver was assessed by reverse transcriptase-polymerase chain reaction (RT-PCR). The HSCs were isolated from mice, and co-cultured with either oleic acid (100 μM) or linoleic acid (100 μM) for 2 days. The expression of alpha-smooth muscle actin (α-SMA) was assessed by immunohistochemistry. In addition, the production of hydroxyproline was determined.

Results

Serum alanine aminotransferase levels and the mRNA expression of TGF-β and collα2 were significantly reduced by treatment of olive oil. Dietary olive oil blunted the expression of α-SMA in the liverand liver injury and hepatic fibrosis were prevented by treatment of olive oil. The number of α-SMA positive cells was significantly lower in HSCs co-cultured with oleic acid than in those co-cultured with linoleic acid. Concentration of hydroxyproline in culture medium was significantly lower in cells co-cultured with oleic acid than in the control.

Conclusions

Dietary olive oil prevents CCl₄-induced tissue injury and fibrosis in the liver. Since oleic acid inhibited activation of HSCs, oleic acid may play a key role on this mechanism.

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Titel: Dietary olive oil prevents carbon tetrachloride-induced hepatic fibrosis in mice
verfasst von: Nobuyuki Tanaka
Hiroshi Kono
Kenichi Ishii
Naohiro Hosomura
Hideki Fujii
Publikationsdatum: 01.09.2009
Verlag: Springer Japan
Erschienen in: Journal of Gastroenterology / Ausgabe 9/2009
Print ISSN: 0944-1174
Elektronische ISSN: 1435-5922
DOI: https://doi.org/10.1007/s00535-009-0088-9

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Dietary olive oil prevents carbon tetrachloride-induced hepatic fibrosis in mice

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