Skip to main content
Erschienen in: European Archives of Oto-Rhino-Laryngology 1/2017

15.07.2016 | Rhinology

Differential protein expression in the secretory fluids of maxillary sinusitis and maxillary retention cyst

verfasst von: Soung Min Kim, Mi Young Eo, Yun Ju Cho, Yeon Sook Kim, Suk Keun Lee

Erschienen in: European Archives of Oto-Rhino-Laryngology | Ausgabe 1/2017

Einloggen, um Zugang zu erhalten

Abstract

Both maxillary sinusitis (MS) and maxillary retention cyst (MRC) involve the maxillary sinus and show similar clinical features. Clinically, differentiating between MS and MRC is sometimes difficult in asymptomatic patients, despite their quite different pathogenic behaviors. To identify differential protein expressions in the secretory fluids of MS and MRC, 25 cases of asymptomatic MS and 15 cases of asymptomatic MRC were examined pathologically in this study. All patients underwent routine endoscopic sinus surgery or modified Caldwell-Luc procedure and the sinus mucosal specimens obtained during these procedures with the approval of the Institutional Review Board. Their secretory fluids were analyzed via immunoprecipitation-based high-performance liquid chromatography (IP-HPLC) using 25 types of antiserum, including inflammatory cytokines, antimicrobial proteins, and mucosal protective proteins. In the histological examinations, MS and MRC showed similar features in the secretory columnar epithelial lining and thick submucosal connective tissue, both of which contained few inflammatory cells infiltrates. The IP-HPLC analysis revealed that TNFα, IL-1, -8, MMP-3, -10, α1-antitrypsin, cathepsin C, lysozyme, lactoferrin, β-defensin-1, -3, LL-37, mucocidin, and mucin-1 were more intensely expressed in MS than in MRC; whereas IgA, cystatin A, and proline-rich proteins were more strongly expressed in MRC than in MS. These data indicate that the secretory fluid of MS is indicative of a more robust inflammatory reaction to certain bacteria compared to that of MRC, while the secretory fluid of MRC contains more abundant mucosal protective proteins compared to that of MS. Taken together, the IP-HPLC analysis of MS and MRC secretory fluid revealed that MRC showed a weaker inflammatory reaction but a stronger mucosal protective function than MS.
Literatur
1.
Zurück zum Zitat Park YW, Kim JH, Kim YH et al (2008) Antral polyp versus maxillary sinusitis. J Korean Assoc Maxillofac Plast Reconstr Surg 30:489–494 Park YW, Kim JH, Kim YH et al (2008) Antral polyp versus maxillary sinusitis. J Korean Assoc Maxillofac Plast Reconstr Surg 30:489–494
2.
Zurück zum Zitat Kim YS, An KY, Lee SK (2010) Pathological findings for mucous retention cyst in maxillary sinus. Korean J Oral Maxillofac Pathol 34:9–18 Kim YS, An KY, Lee SK (2010) Pathological findings for mucous retention cyst in maxillary sinus. Korean J Oral Maxillofac Pathol 34:9–18
3.
Zurück zum Zitat Donizeth-Rodrigues C, Fonseca-Da Silveira M, Goncalves-De Alencar AH et al (2013) Three-dimensional images contribute to the diagnosis of mucous retention cyst in maxillary sinus. Med Oral Patol Oral Cir Bucal 18:e151–e157CrossRefPubMed Donizeth-Rodrigues C, Fonseca-Da Silveira M, Goncalves-De Alencar AH et al (2013) Three-dimensional images contribute to the diagnosis of mucous retention cyst in maxillary sinus. Med Oral Patol Oral Cir Bucal 18:e151–e157CrossRefPubMed
4.
Zurück zum Zitat Benevides GN, Salgado GA Jr, Ferreira CR et al (2015) Bacterial sinusitis and its frightening complications: subdural empyema and Lemierre syndrome. Autopsy Case Rep 5:19–26CrossRef Benevides GN, Salgado GA Jr, Ferreira CR et al (2015) Bacterial sinusitis and its frightening complications: subdural empyema and Lemierre syndrome. Autopsy Case Rep 5:19–26CrossRef
5.
Zurück zum Zitat Banglawala SM, Schlosser RJ, Wenztel J et al (2016) Trends in chronic rhinosinusitis research in the past three decades. Int Forum Allergy Rhinol 6:46–51CrossRefPubMed Banglawala SM, Schlosser RJ, Wenztel J et al (2016) Trends in chronic rhinosinusitis research in the past three decades. Int Forum Allergy Rhinol 6:46–51CrossRefPubMed
6.
Zurück zum Zitat Gardner DG (1984) Pseudocysts and retention cysts of the maxillary sinus. Oral Surg Oral Med Oral Pathol 58:561–567CrossRefPubMed Gardner DG (1984) Pseudocysts and retention cysts of the maxillary sinus. Oral Surg Oral Med Oral Pathol 58:561–567CrossRefPubMed
7.
Zurück zum Zitat Habesoglu TE, Habesoglu M, Surmeli M et al (2010) Unilateral sinonasal symptoms. J Craniofac Surg 21:2019–2022CrossRefPubMed Habesoglu TE, Habesoglu M, Surmeli M et al (2010) Unilateral sinonasal symptoms. J Craniofac Surg 21:2019–2022CrossRefPubMed
8.
Zurück zum Zitat Marcal Vieira EM, de Morais S, de Musis CR et al (2015) Frequency of maxillary sinus mucous retention cysts in a central Brazilian population. J Dent (Shiraz) 16:169–174 Marcal Vieira EM, de Morais S, de Musis CR et al (2015) Frequency of maxillary sinus mucous retention cysts in a central Brazilian population. J Dent (Shiraz) 16:169–174
9.
Zurück zum Zitat Rodrigues CD, Freire GF, Silva LB et al (2009) Prevalence and risk factors of mucous retention cysts in a Brazilian population. Dentomaxillofac Radiol 38:480–483CrossRefPubMed Rodrigues CD, Freire GF, Silva LB et al (2009) Prevalence and risk factors of mucous retention cysts in a Brazilian population. Dentomaxillofac Radiol 38:480–483CrossRefPubMed
10.
Zurück zum Zitat Lee JT, Escobar OH, Anouseyan R et al (2014) Assessment of epithelial innate antimicrobial factors in sinus tissue from patients with and without chronic rhinosinusitis. Int Forum Allergy Rhinol 4:893–900CrossRefPubMedPubMedCentral Lee JT, Escobar OH, Anouseyan R et al (2014) Assessment of epithelial innate antimicrobial factors in sinus tissue from patients with and without chronic rhinosinusitis. Int Forum Allergy Rhinol 4:893–900CrossRefPubMedPubMedCentral
11.
Zurück zum Zitat Zustin J, Scheuer HA, Knecht R (2013) Enhanced podoplanin expression in chronic maxillary sinusitis. In Vivo 27:551–554PubMed Zustin J, Scheuer HA, Knecht R (2013) Enhanced podoplanin expression in chronic maxillary sinusitis. In Vivo 27:551–554PubMed
12.
Zurück zum Zitat Kim YS (2015) Protein expression changes induced by cisplatin in an oral cancer cell line as determined by immunoprecipitation-based high performance liquid chromatography. Korean J Oral Maxillofac Pathol 39:567–582CrossRef Kim YS (2015) Protein expression changes induced by cisplatin in an oral cancer cell line as determined by immunoprecipitation-based high performance liquid chromatography. Korean J Oral Maxillofac Pathol 39:567–582CrossRef
13.
Zurück zum Zitat Kim YS, Lee SK (2015) IP-HPLC analysis of human salivary protein complexes. Korean J Oral Maxillofac Pathol 39:615–622CrossRef Kim YS, Lee SK (2015) IP-HPLC analysis of human salivary protein complexes. Korean J Oral Maxillofac Pathol 39:615–622CrossRef
14.
Zurück zum Zitat Kim YS, Lee SK, Park SC et al (2005) Cloning and identification of a new antimicrobial protein, salvic, from human salivary gland. In: International symposium of Maxillofacial and Oral Regenerative Biology in Okayama, vol 1, pp 258–260 Kim YS, Lee SK, Park SC et al (2005) Cloning and identification of a new antimicrobial protein, salvic, from human salivary gland. In: International symposium of Maxillofacial and Oral Regenerative Biology in Okayama, vol 1, pp 258–260
15.
Zurück zum Zitat Kim YS, Lim JG, Lee SS et al (2006) Polyclonal multiple antibodies against proteins cross-linked on oral exfoliated squamous cells. Korean J Gerontol 16:164–170 Kim YS, Lim JG, Lee SS et al (2006) Polyclonal multiple antibodies against proteins cross-linked on oral exfoliated squamous cells. Korean J Gerontol 16:164–170
16.
Zurück zum Zitat Kim YS, Lee SK (2009) Aggregation of salivary mucin-1 and proline rich proteins by transglutaminase 4 in a huge sialolith with central nidus of bacterial colony. Korean J Oral Maxillofac Pathol 33:239–244 Kim YS, Lee SK (2009) Aggregation of salivary mucin-1 and proline rich proteins by transglutaminase 4 in a huge sialolith with central nidus of bacterial colony. Korean J Oral Maxillofac Pathol 33:239–244
17.
Zurück zum Zitat Daele J, Zicot AF (2000) Humoral immunodeficiency in recurrent upper respiratory tract infections: some basic, clinical and therapeutic features. Acta Otorhinolaryngol Belg 54:373–390PubMed Daele J, Zicot AF (2000) Humoral immunodeficiency in recurrent upper respiratory tract infections: some basic, clinical and therapeutic features. Acta Otorhinolaryngol Belg 54:373–390PubMed
18.
Zurück zum Zitat Xu JH, Dai WJ, Chen B et al (2015) Mucosal immunization with PsaA protein, using chitosan as a delivery system, increases protection against acute otitis media and invasive infection by Streptococcus pneumoniae. Scand J Immunol 81:177–185CrossRefPubMed Xu JH, Dai WJ, Chen B et al (2015) Mucosal immunization with PsaA protein, using chitosan as a delivery system, increases protection against acute otitis media and invasive infection by Streptococcus pneumoniae. Scand J Immunol 81:177–185CrossRefPubMed
19.
Zurück zum Zitat Carothers DG, Graham SM, Jia HP et al (2001) Production of beta-defensin antimicrobial peptides by maxillary sinus mucosa. Am J Rhinol 15:175–179CrossRefPubMed Carothers DG, Graham SM, Jia HP et al (2001) Production of beta-defensin antimicrobial peptides by maxillary sinus mucosa. Am J Rhinol 15:175–179CrossRefPubMed
20.
Zurück zum Zitat Kaliner MA (1992) Human nasal host defense and sinusitis. J Allergy Clin Immunol 90(3 Pt 2):424–430CrossRefPubMed Kaliner MA (1992) Human nasal host defense and sinusitis. J Allergy Clin Immunol 90(3 Pt 2):424–430CrossRefPubMed
21.
Zurück zum Zitat Stoeckelhuber M, Olzowy B, Ihler F et al (2014) Immunolocalization of antimicrobial and cytoskeletal components in the serous glands of human sinonasal mucosa. Histol Histopathol 29:1315–1324PubMed Stoeckelhuber M, Olzowy B, Ihler F et al (2014) Immunolocalization of antimicrobial and cytoskeletal components in the serous glands of human sinonasal mucosa. Histol Histopathol 29:1315–1324PubMed
22.
Zurück zum Zitat Woods CM, Lee VS, Hussey DJ et al (2012) Lysozyme expression is increased in the sinus mucosa of patients with chronic rhinosinusitis. Rhinology 50:147–156PubMed Woods CM, Lee VS, Hussey DJ et al (2012) Lysozyme expression is increased in the sinus mucosa of patients with chronic rhinosinusitis. Rhinology 50:147–156PubMed
23.
Zurück zum Zitat Jyonouchi H, Sun S, Kennedy CA et al (2000) Interferon gamma levels in the sinus, ear, and airway in a rabbit sinusitis model induced by Bacteroides inoculation. Arch Otolaryngol Head Neck Surg 126:529–532CrossRefPubMed Jyonouchi H, Sun S, Kennedy CA et al (2000) Interferon gamma levels in the sinus, ear, and airway in a rabbit sinusitis model induced by Bacteroides inoculation. Arch Otolaryngol Head Neck Surg 126:529–532CrossRefPubMed
24.
Zurück zum Zitat Elmorsy S, El-Naggar MM, Abdel-Aal SM et al (2010) Sinus aspirates in chronic rhinosinusitis: fungal colonization of paranasal sinuses, evaluation of ICAM-1 and IL-8 and studying of immunological effect of long-term macrolide therapy. Rhinology 48:312–317PubMed Elmorsy S, El-Naggar MM, Abdel-Aal SM et al (2010) Sinus aspirates in chronic rhinosinusitis: fungal colonization of paranasal sinuses, evaluation of ICAM-1 and IL-8 and studying of immunological effect of long-term macrolide therapy. Rhinology 48:312–317PubMed
25.
Zurück zum Zitat Tokushige E, Itoh K, Ushikai M et al (1994) Localization of IL-1 beta mRNA and cell adhesion molecules in the maxillary sinus mucosa of patients with chronic sinusitis. Laryngoscope 104:1245–1250CrossRefPubMed Tokushige E, Itoh K, Ushikai M et al (1994) Localization of IL-1 beta mRNA and cell adhesion molecules in the maxillary sinus mucosa of patients with chronic sinusitis. Laryngoscope 104:1245–1250CrossRefPubMed
26.
Zurück zum Zitat Detwiller KY, Smith TL, Mace JC et al (2013) Steroid-independent upregulation of matrix metalloproteinase 9 in chronic rhinosinusitis patients with radiographic evidence of osteitis. Int Forum Allergy Rhinol 3:364–368CrossRefPubMedPubMedCentral Detwiller KY, Smith TL, Mace JC et al (2013) Steroid-independent upregulation of matrix metalloproteinase 9 in chronic rhinosinusitis patients with radiographic evidence of osteitis. Int Forum Allergy Rhinol 3:364–368CrossRefPubMedPubMedCentral
27.
Zurück zum Zitat Hamaguchi Y, Ohi M, Sakakura Y et al (1986) Significance of lysosomal proteases; cathepsins B and H in maxillary mucosa and nasal polyp with non-atopic chronic inflammation. Rhinology 24:187–194PubMed Hamaguchi Y, Ohi M, Sakakura Y et al (1986) Significance of lysosomal proteases; cathepsins B and H in maxillary mucosa and nasal polyp with non-atopic chronic inflammation. Rhinology 24:187–194PubMed
28.
Zurück zum Zitat Kay NJ, Terry RM, Swinburne L (1986) Protease inhibitors in maxillary antral mucosa. J Laryngol Otol 100:289–290CrossRefPubMed Kay NJ, Terry RM, Swinburne L (1986) Protease inhibitors in maxillary antral mucosa. J Laryngol Otol 100:289–290CrossRefPubMed
29.
Zurück zum Zitat Lee CW, Kim TH, Lee HM et al (2009) Upregulation of elafin and cystatin C in the ethmoid sinus mucosa of patients with chronic sinusitis. Arch Otolaryngol Head Neck Surg 135:771–775CrossRefPubMed Lee CW, Kim TH, Lee HM et al (2009) Upregulation of elafin and cystatin C in the ethmoid sinus mucosa of patients with chronic sinusitis. Arch Otolaryngol Head Neck Surg 135:771–775CrossRefPubMed
30.
Zurück zum Zitat Woods CM, Hooper DN, Ooi EH et al (2012) Fungicidal activity of lysozyme is inhibited in vitro by commercial sinus irrigation solutions. Am J Rhinol Allergy 26:298–301CrossRefPubMed Woods CM, Hooper DN, Ooi EH et al (2012) Fungicidal activity of lysozyme is inhibited in vitro by commercial sinus irrigation solutions. Am J Rhinol Allergy 26:298–301CrossRefPubMed
31.
Zurück zum Zitat Woods CM, Hooper DN, Ooi EH et al (2011) Human lysozyme has fungicidal activity against nasal fungi. Am J Rhinol Allergy 25:236–240CrossRefPubMed Woods CM, Hooper DN, Ooi EH et al (2011) Human lysozyme has fungicidal activity against nasal fungi. Am J Rhinol Allergy 25:236–240CrossRefPubMed
32.
Zurück zum Zitat Seshadri S, Lin DC, Rosati M et al (2012) Reduced expression of antimicrobial PLUNC proteins in nasal polyp tissues of patients with chronic rhinosinusitis. Allergy 67:920–928CrossRefPubMedPubMedCentral Seshadri S, Lin DC, Rosati M et al (2012) Reduced expression of antimicrobial PLUNC proteins in nasal polyp tissues of patients with chronic rhinosinusitis. Allergy 67:920–928CrossRefPubMedPubMedCentral
33.
Zurück zum Zitat Claeys S, de Belder T, Holtappels G et al (2003) Human beta-defensins and toll-like receptors in the upper airway. Allergy 58:748–753CrossRefPubMed Claeys S, de Belder T, Holtappels G et al (2003) Human beta-defensins and toll-like receptors in the upper airway. Allergy 58:748–753CrossRefPubMed
34.
Zurück zum Zitat Chennupati SK, Chiu AG, Tamashiro E et al (2009) Effects of an LL-37-derived antimicrobial peptide in an animal model of biofilm Pseudomonas sinusitis. Am J Rhinol Allergy 23:46–51CrossRefPubMed Chennupati SK, Chiu AG, Tamashiro E et al (2009) Effects of an LL-37-derived antimicrobial peptide in an animal model of biofilm Pseudomonas sinusitis. Am J Rhinol Allergy 23:46–51CrossRefPubMed
35.
Zurück zum Zitat Takeuchi K, Yuta A, Sakakura Y (1995) MUC2 mucin gene expression in the nose and maxillary sinus. Am J Otolaryngol 16:391–395CrossRefPubMed Takeuchi K, Yuta A, Sakakura Y (1995) MUC2 mucin gene expression in the nose and maxillary sinus. Am J Otolaryngol 16:391–395CrossRefPubMed
36.
Zurück zum Zitat Sakurada K, Akutsu T, Watanabe K et al (2012) Identification of nasal blood by real-time RT-PCR. Leg Med (Tokyo) 14:201–204CrossRef Sakurada K, Akutsu T, Watanabe K et al (2012) Identification of nasal blood by real-time RT-PCR. Leg Med (Tokyo) 14:201–204CrossRef
37.
Zurück zum Zitat Carenfelt C (1977) Maxillary sinusitis. Effects of treatment on the local antibacterial defence. Acta Otolaryngol 84:440–445CrossRefPubMed Carenfelt C (1977) Maxillary sinusitis. Effects of treatment on the local antibacterial defence. Acta Otolaryngol 84:440–445CrossRefPubMed
38.
Zurück zum Zitat Tesfaigzi J, Th’ng J, Hotchkiss JA et al (1996) A small proline-rich protein, SPRR1, is upregulated early during tobacco smoke-induced squamous metaplasia in rat nasal epithelia. Am J Respir Cell Mol Biol 14:478–486CrossRefPubMed Tesfaigzi J, Th’ng J, Hotchkiss JA et al (1996) A small proline-rich protein, SPRR1, is upregulated early during tobacco smoke-induced squamous metaplasia in rat nasal epithelia. Am J Respir Cell Mol Biol 14:478–486CrossRefPubMed
39.
Zurück zum Zitat Warner TF, Azen EA (1984) Proline-rich proteins are present in serous cells of submucosal glands in the respiratory tract. Am Rev Respir Dis 130:115–118PubMed Warner TF, Azen EA (1984) Proline-rich proteins are present in serous cells of submucosal glands in the respiratory tract. Am Rev Respir Dis 130:115–118PubMed
40.
Zurück zum Zitat Takabayashi T, Kato A, Peters AT et al (2013) Increased expression of factor XIII-A in patients with chronic rhinosinusitis with nasal polyps. J Allergy ClinImmunol 132:584–592CrossRef Takabayashi T, Kato A, Peters AT et al (2013) Increased expression of factor XIII-A in patients with chronic rhinosinusitis with nasal polyps. J Allergy ClinImmunol 132:584–592CrossRef
41.
Zurück zum Zitat Albu S (2010) Symptomatic maxillary sinus retention cysts: should they be removed? Laryngoscope 120:1904–1909CrossRefPubMed Albu S (2010) Symptomatic maxillary sinus retention cysts: should they be removed? Laryngoscope 120:1904–1909CrossRefPubMed
Metadaten
Titel
Differential protein expression in the secretory fluids of maxillary sinusitis and maxillary retention cyst
verfasst von
Soung Min Kim
Mi Young Eo
Yun Ju Cho
Yeon Sook Kim
Suk Keun Lee
Publikationsdatum
15.07.2016
Verlag
Springer Berlin Heidelberg
Erschienen in
European Archives of Oto-Rhino-Laryngology / Ausgabe 1/2017
Print ISSN: 0937-4477
Elektronische ISSN: 1434-4726
DOI
https://doi.org/10.1007/s00405-016-4167-2

Weitere Artikel der Ausgabe 1/2017

European Archives of Oto-Rhino-Laryngology 1/2017 Zur Ausgabe

Update HNO

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.