Introduction
Experimental procedures
Cell lines and cell culture
Antibodies
Fluorescence microscopy
Flow cytometry analysis
Differentiation of iPS cells
Western blot
Results
Characterization of human iPS cells
Dose-dependent responses of human iPS and Tera-1 cells to Cr(VI)
Time-dependent response of human iPS, Tera-1, and BEAS-2B cells to Cr(VI)
Time-dependent response of human iPS cells, Tera-1, and BEAS-2B cells to H2O2
Time-dependent response of human iPS cells, Tera-1, and BEAS-2B cells to Dox
Cell type | Treatment | Total p53 | p-ATM | γH2AX | p-p53S15 | p-p53S20 | p-p53S392 |
---|---|---|---|---|---|---|---|
iPS cells | Cr(VI) | Up early down late | Biphasic induction | Strongly up late | Unchanged | Undetectable | Undetectable |
10 μM | |||||||
H2O2 | Slightly up early and down late | Biphasic induction | Strongly up late | Strongly up early and down late | Undetectable | Slightly up early and down late | |
100 μM | |||||||
Dox | Slightly up over time | Up over time | Up late | Up early and down late | Undetectable | Strongly up early and down late | |
250 nM | |||||||
Tera-1 | Cr(VI) | Slightly up over time | Up late | Strongly up late | Strongly up overtime | Undetectable | Strongly up over time |
10 μM | |||||||
H2O2 | Slightly up over time | Up early and down late | Undetectable | Unchanged | Up over time and down late | Up early and stays up | |
100 μM | |||||||
Dox | Significantly up over time | Significantly up early and down late | Strongly up at 8 h and down late | Strongly up late | Slightly up late | Strongly up over time | |
250 nM | |||||||
BEASE-2B | Cr(VI) | Unchanged | Slightly up over time | Strongly up late | Up over time | Up over time | Unchanged |
10 μM | |||||||
H2O2 | Unchanged | Largely unchanged | Undetectable | Largely unchanged | Largely unchanged | Largely unchanged | |
100 μM | |||||||
Dox | Unchanged | Up early and down late | Strongly up late | Strongly up early and down late | Strongly up early and down late | Unchanged up to 24 h and down at 48 h | |
250 nM |