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Erschienen in: Journal of Parasitic Diseases 3/2016

20.09.2014 | Original Article

Digenetic larvae in Schistosome snails from El Fayoum, Egypt with detection of Schistosoma mansoni in the snail by PCR

verfasst von: Shawky M Aboelhadid, Marwa Thabet, Dayhoum El-Basel, Ragaa Taha

Erschienen in: Journal of Parasitic Diseases | Ausgabe 3/2016

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Abstract

The present study aims to detect the digenetic larvae infections in Bulinus truncatus and Biomphalaria alexandrina snails and also PCR detection of Schistosoma mansoni infection. The snails were collected from different branches of Yousef canal and their derivatives in El Fayoum Governorate. The snails were investigated for infection through induction of cercarial shedding by exposure to light and crushing of the snails. The shed cercariae were S. mansoni, Pharyngeate longifurcate type I and Pharyngeate longifurcate type II from B. alexandrina, while that found in B. truncatus were Schitosoma haematobium and Xiphidiocercaria species cercariae. The seasonal prevalence of infection was discussed. Polymerase chain reaction was used for the detection of S. mansoni in the DNA from field collected infected and non infected snails. The results of PCR showed that the pool of B. alexandrina snails which shed S. mansoni cercariae in the laboratory, gave positive reaction in the samples. Pooled samples of field collected B. alexandrina that showed negative microscopic shedding of cercariae gave negative and positive PCR in a consecutive manner. Accordingly, a latent infection in the snail (negative microscopic) could be detected by using PCR.
Literatur
Zurück zum Zitat Abdel Ghany AF (1955) Studies on the life cycle of trematodes of egyptian domesticated animals, Ph.D. Thesis, Fac. Vet. Med., Cairo University Abdel Ghany AF (1955) Studies on the life cycle of trematodes of egyptian domesticated animals, Ph.D. Thesis, Fac. Vet. Med., Cairo University
Zurück zum Zitat Abdella MA, Heluy MB, Magdy TK (1999) Freshwater molluscs of Egypt, vol 10. Publication of National Biodiversity Unit, Cairo Abdella MA, Heluy MB, Magdy TK (1999) Freshwater molluscs of Egypt, vol 10. Publication of National Biodiversity Unit, Cairo
Zurück zum Zitat Aboelhadid, SM (2004) Biological studies on parasites transmitted from some freshwater snails to other hosts, Ph.D. Thesis, Faculty of Veterinary Medicine, Beni-Suef, Cairo University Aboelhadid, SM (2004) Biological studies on parasites transmitted from some freshwater snails to other hosts, Ph.D. Thesis, Faculty of Veterinary Medicine, Beni-Suef, Cairo University
Zurück zum Zitat Abou Basha LM, Salem A, Allam A, Loutfy NF, Bassioni HK (1989) Study on the morphology and identification of the various trematode infections detected in Biomphalaria alexandrina in the vicinity of Alexanderia. J Med Res Inst 10:1 Abou Basha LM, Salem A, Allam A, Loutfy NF, Bassioni HK (1989) Study on the morphology and identification of the various trematode infections detected in Biomphalaria alexandrina in the vicinity of Alexanderia. J Med Res Inst 10:1
Zurück zum Zitat Cable RM (1977) An illustrated laboratory manual of parasitology, 5th edn. Burgess publishing Company, Minneapolis Cable RM (1977) An illustrated laboratory manual of parasitology, 5th edn. Burgess publishing Company, Minneapolis
Zurück zum Zitat Chingwena G, Mukaratirwa S, Kristensen TK, Chimbari M (2002) Susceptibility of freshwater snails to the amphistome Calicophoron microbothrium and the influence of the species on susceptibility of Bulinus tropicus to Schistosoma haematobium and Schistosoma mattheei infections. J Parasitol 88:880–883CrossRefPubMed Chingwena G, Mukaratirwa S, Kristensen TK, Chimbari M (2002) Susceptibility of freshwater snails to the amphistome Calicophoron microbothrium and the influence of the species on susceptibility of Bulinus tropicus to Schistosoma haematobium and Schistosoma mattheei infections. J Parasitol 88:880–883CrossRefPubMed
Zurück zum Zitat Doumenge JP, Mott KE, Cheung C, Villenave D, Chapuis O, Perrin MF, Reaud TG (1987) Atlas of the global distribution of schistosomiasis. Talence, CEGET-CNRS. World Health Organisation, Geneva Doumenge JP, Mott KE, Cheung C, Villenave D, Chapuis O, Perrin MF, Reaud TG (1987) Atlas of the global distribution of schistosomiasis. Talence, CEGET-CNRS. World Health Organisation, Geneva
Zurück zum Zitat Fathalla A (1981) Egyptian freshwater snails and the larval trematode librated from it, Ph.D. Thesis Faculty of Medicine, Ain Shams University Fathalla A (1981) Egyptian freshwater snails and the larval trematode librated from it, Ph.D. Thesis Faculty of Medicine, Ain Shams University
Zurück zum Zitat Frandsen F, Christensen NQ (1984) An introductory guide to the identification of cercariae from freshwater snails with special reference to cercariae of trematode species of medical and veterinary importance. Acta Trop 41:181–202PubMed Frandsen F, Christensen NQ (1984) An introductory guide to the identification of cercariae from freshwater snails with special reference to cercariae of trematode species of medical and veterinary importance. Acta Trop 41:181–202PubMed
Zurück zum Zitat Hamburger J, He N, Xin XY, Ramzy RM, Jourdane J, Ruppel A (1998) A polymerase chain reaction assay for detecting snails infected with bilharzia parasites (Schistosoma mansoni) from very early prepatency. Am J Trop Med Hyg 59:872–876CrossRef Hamburger J, He N, Xin XY, Ramzy RM, Jourdane J, Ruppel A (1998) A polymerase chain reaction assay for detecting snails infected with bilharzia parasites (Schistosoma mansoni) from very early prepatency. Am J Trop Med Hyg 59:872–876CrossRef
Zurück zum Zitat Hanelt B, Adema CM, Mansour MH, Loker ES (1997) Detection of Schistosoma mansoni in Biomphalaria using nested PCR. J Parasitol 83:387–394CrossRefPubMed Hanelt B, Adema CM, Mansour MH, Loker ES (1997) Detection of Schistosoma mansoni in Biomphalaria using nested PCR. J Parasitol 83:387–394CrossRefPubMed
Zurück zum Zitat Hassan IM (1987) Studies on the role played by some snails in transmitting parasites to animals and man in Qena Province, Ph.D. Thesis, Qena, Faculty of Science, Assuit University Hassan IM (1987) Studies on the role played by some snails in transmitting parasites to animals and man in Qena Province, Ph.D. Thesis, Qena, Faculty of Science, Assuit University
Zurück zum Zitat Hertel J, Haberl B, Hamburguer JHW (2003) Description of a tandem repeated DNA sequence of Echinostoma caproni and methods for its detection in snail and plankton samples. Parasitology 126:443–449CrossRefPubMed Hertel J, Haberl B, Hamburguer JHW (2003) Description of a tandem repeated DNA sequence of Echinostoma caproni and methods for its detection in snail and plankton samples. Parasitology 126:443–449CrossRefPubMed
Zurück zum Zitat Jackson JH (1958) Bilharezia, a back ground of its epedemicity and control in Africa with particular reference to irrigation scheme. South Afr J Lab Clin Med 4:1–54 Jackson JH (1958) Bilharezia, a back ground of its epedemicity and control in Africa with particular reference to irrigation scheme. South Afr J Lab Clin Med 4:1–54
Zurück zum Zitat Khalifa RM, Saad AI, Maher NH (1997) Studies on some freshwater cercariae from Aswan-Egypt: Part I. J Egypt Ger Soc Zool 24:225–246 Khalifa RM, Saad AI, Maher NH (1997) Studies on some freshwater cercariae from Aswan-Egypt: Part I. J Egypt Ger Soc Zool 24:225–246
Zurück zum Zitat Korany HA (2011) Studies on some parasites infecting some Freshwater snails at Beni-Suef Governorate, M.Sc. thesis, Faculty of Science, Beni-Suef University, Egypt Korany HA (2011) Studies on some parasites infecting some Freshwater snails at Beni-Suef Governorate, M.Sc. thesis, Faculty of Science, Beni-Suef University, Egypt
Zurück zum Zitat Mandahl-Barth G (1958) Intermediate hosts of Schistosoma: African Biomphalaria and Bulins Genera. World Health Organization Monographs. Ser. No. 37 Mandahl-Barth G (1958) Intermediate hosts of Schistosoma: African Biomphalaria and Bulins Genera. World Health Organization Monographs. Ser. No. 37
Zurück zum Zitat Mandahl-Barth G (1962) Key to the identification of East and Central African freshwater snails of medical and veterinary importance. Bull World Health Org 27(1):135–150PubMed Mandahl-Barth G (1962) Key to the identification of East and Central African freshwater snails of medical and veterinary importance. Bull World Health Org 27(1):135–150PubMed
Zurück zum Zitat Melo FL, Vale-Gomes AL, Barbosa CS, Werkhauser RP, Abath FGC (2006) Development of molecular approaches for the identification of transmission sites of schistosomiasis. Trans R Soc Trop Med Hyg 100:1049–1055CrossRef Melo FL, Vale-Gomes AL, Barbosa CS, Werkhauser RP, Abath FGC (2006) Development of molecular approaches for the identification of transmission sites of schistosomiasis. Trans R Soc Trop Med Hyg 100:1049–1055CrossRef
Zurück zum Zitat Rifaat MA, Ismail IM, EI-Mahallawy MN, Schafiaa AS (1971) The use of non-schistosome antigens in the fluorescent antibody technique for the diagnosis of schistosomiasis. J Egypt Soc Parasitol I 2:103–109 Rifaat MA, Ismail IM, EI-Mahallawy MN, Schafiaa AS (1971) The use of non-schistosome antigens in the fluorescent antibody technique for the diagnosis of schistosomiasis. J Egypt Soc Parasitol I 2:103–109
Zurück zum Zitat Thiengo SC, Mattos AC, Boaventura FM, Loureiro MS, Santos SB, Fernandez MA (2004) Freshwater snails and schistosomiasis mansoni in the state of Rio de Janeiro, Brazil: V: Norte Fluminense Mesoregion. Mem Inst Oswaldo Cruz 99:99–103CrossRefPubMed Thiengo SC, Mattos AC, Boaventura FM, Loureiro MS, Santos SB, Fernandez MA (2004) Freshwater snails and schistosomiasis mansoni in the state of Rio de Janeiro, Brazil: V: Norte Fluminense Mesoregion. Mem Inst Oswaldo Cruz 99:99–103CrossRefPubMed
Zurück zum Zitat Vidigal THDA, Caldeira RL, Simpson AJG, Carvalho OS (2000) Further studies on the molecular systematics of Biomphalaria snails from Brazil. Mem Inst Oswaldo Cruz 95:57–66CrossRefPubMed Vidigal THDA, Caldeira RL, Simpson AJG, Carvalho OS (2000) Further studies on the molecular systematics of Biomphalaria snails from Brazil. Mem Inst Oswaldo Cruz 95:57–66CrossRefPubMed
Zurück zum Zitat Wanas MQA, Abou-Senna FM, Al-Shareef AMF (1993) Studies on larval digenetic trematodes of Xiphidiocercaria from some Egyptian freshwater snails. J Egypt Soc Parasitol 23:829–851PubMed Wanas MQA, Abou-Senna FM, Al-Shareef AMF (1993) Studies on larval digenetic trematodes of Xiphidiocercaria from some Egyptian freshwater snails. J Egypt Soc Parasitol 23:829–851PubMed
Zurück zum Zitat WHO (World Health Organization) (1993) The control of schistosomiasis. WHO Technical Report Series 830. Geneva WHO (World Health Organization) (1993) The control of schistosomiasis. WHO Technical Report Series 830. Geneva
Metadaten
Titel
Digenetic larvae in Schistosome snails from El Fayoum, Egypt with detection of Schistosoma mansoni in the snail by PCR
verfasst von
Shawky M Aboelhadid
Marwa Thabet
Dayhoum El-Basel
Ragaa Taha
Publikationsdatum
20.09.2014
Verlag
Springer India
Erschienen in
Journal of Parasitic Diseases / Ausgabe 3/2016
Print ISSN: 0971-7196
Elektronische ISSN: 0975-0703
DOI
https://doi.org/10.1007/s12639-014-0567-7

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