Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Klimawandel und Gesundheit Neues aus dem Markt COVID-19 Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Konkret geht es um den Diabetes-Patienten mit kardiovaskulären Erkrankungen oder Risiken, die Herzinsuffizienz sowie die kardiovaskuläre Primär- und Sekundärprävention. Sind Sie hier schon auf dem neuesten Stand? Unsere Experten beleuchten, wo und warum das bisherige Procedere geändert werden soll und was in der Praxis sinnvoll ist. Holen Sie sich Ihr Update und 2 CME-Punkte beim MMW-Webinar am 24. April von 17.30 bis 19 Uhr. 
Erweiterte Suche
Anmelden
nach oben
Cardiovascular Toxicology

17.08.2017

Diosmin Prevents Isoproterenol-Induced Heart Mitochondrial Oxidative Stress in Rats

verfasst von: S. Sharmila Queenthy, P. Stanely Mainzen Prince, Babu John

Erschienen in: Cardiovascular Toxicology | Ausgabe 2/2018

Einloggen, um Zugang zu erhalten

Abstract

Cardiac mitochondrial oxidative stress causes mitochondrial damage that plays an important role in the pathology of myocardial infarction. The preventive effects of diosmin on cardiac mitochondrial oxidative stress in isoproterenol-induced myocardial infarcted rats were evaluated. Rats were pretreated with diosmin (10 mg/kg body weight) daily for 10 days. Myocardial infarction was induced in rats by isoproterenol (100 mg/kg body weight) injection twice at an interval of 24 h (on 11th and 12th day). Isoproterenol-induced myocardial infarcted rats showed a significant increase in the levels of cardiac diagnostic markers, heart mitochondrial lipid peroxidation, calcium ion, and a significant decrease in the levels of heart mitochondrial glutathione peroxidase, reduced glutathione, glutathione-S-transferase, isocitrate, malate, α-ketoglutarate, and succinate dehydrogenases. Transmission electron microscopic findings revealed damaged mitochondria with loss of cristae, swelling, and vacuolation in isoproterenol-induced rats’ heart. Diosmin pretreatment showed significant preventive effects on all the biochemical parameters, and the structure of mitochondria was evaluated. Furthermore, the transmission electron microscopic study confirms the biochemical findings. The antioxidant and negative inotropic effects of diosmin inhibited cardiac mitochondrial oxidative stress and prevented mitochondrial damage in myocardial infarcted rats.
Literatur
1.
Rajadurai, M., & Stanely Mainzen Prince, P. (2007). Preventive effect of naringin on isoproterenol induced cardiotoxicity in Wistar rats: An in vivo and in vitro study. Toxicology, 232, 216–225.CrossRefPubMed
2.
Wang, J., Bo, H., Meng, X., Wu, Y., Bao, Y., & Li, Y. (2006). A simple and fast experimental model of myocardial infarction in the mouse. Texas Heart Institute Journal, 33, 290–293.PubMedPubMedCentral
3.
Rathore, N., John, S., Kale, M., & Bhatnagar, D. (1998). Lipid peroxidation and antioxidant enzymes in isoproterenol induced oxidative stress in rat tissues. Pharmacological Research, 38, 97–303.CrossRef
4.
Srivastava, S., Chandrasekar, B., Gu, Y., Luo, J., Hamid, T., Hill, B. G., et al. (2007). Down regulation of CuZn-superoxide dismutase contributes to β-adrenergic receptor-mediated oxidative stress in the heart. Cardiovascular Research, 74, 445–455.CrossRefPubMed
5.
Rajadurai, M., & Stanely Mainzen Prince, P. (2006). Preventive effect of naringin on lipid peroxides and antioxidants in isoproterenol-induced cardiotoxicity in Wistar rats: Biochemical and histopathological evidences. Toxicology, 228, 259–268.CrossRefPubMed
6.
Senthil Kumaran, K., & Stanely Mainzen Prince, P. (2010). Caffeic acid protects rat heart mitochondria against isoproterenol-induced oxidative damage. Cell Stress and Chaperones, 15, 791–806.CrossRef
7.
Ho, C. T., Osawa, T., Huang, M. T., & Rosen, R. T. (Eds.) (1994). Food phytochemicals and cancer preventions II. Teas, Spices and Herbs, ACS symposium series 547, American Chemical Society, Washington, DC.
8.
Sharmila Queenthy, S., & John, Babu. (2013). Diosmin exhibits anti-hyperlipidemic effects in isoproterenol induced myocardial infarcted rats. European Journal of Pharmacology, 718, 213–218.CrossRefPubMed
9.
Kuntz, S., Wenzel, U., & Daniel, H. (1999). Comparative analysis of the effects of flavonoids on proliferation, cytotoxicity and apoptosis in human colon cancer cell lines. European Journal of Nutrition, 38, 133–142.CrossRefPubMed
10.
Murray, A. J., Edwards, L. M., & Clarke, K. (2007). Mitochondria and heart failure. Current Opinion in Clinical Nutrition and Metabolic Care, 10, 704–711.CrossRefPubMed
11.
Stanely Mainzen Prince, P. (2011). A biochemical, electrocardiographic, electrophoretic, histopathological and in vitro study on the protective effects of (–) epicatechin in isoproterenol induced myocardial infarcted rats. European Journal of Pharmacology, 67, 195–201.
12.
Takasawa, M., Hayakawa, M., Sugiyama, S., Hattori, K., Ito, T., & Ozawa, T. (1993). Age-associated damage in mitochondrial function in rat hearts. Experimental Gerontology, 28, 269–280.CrossRefPubMed
13.
Fraga, C. G., Leibovitz, B. E., & Tappel, A. L. (1988). Lipid peroxidation measured as thiobarbituric acid reactive substances in tissue slices: Characterization and comparison with homogenate and microsomes. Free Radical Biology and Medicine, 4, 155–161.CrossRefPubMed
14.
Kakkar, P., Das, B., & Viswanathan, P. N. (1984). A modified spectrophotometric assay of superoxide dismutase. Indian Journal of Biochemistry and Biophysics, 21, 130–132.PubMed
15.
Rotruck, J. T., Pope, A. L., Ganther, H. E., Swanson, A. B., Hafeman, D. G., & Hoekstra, W. G. (1973). Selenium: Biochemical role as a component of glutathione peroxidase. Science, 179, 588–590.CrossRefPubMed
16.
Ellman, G. L. (1959). Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics, 82, 70–77.CrossRefPubMed
17.
Habig, W. H., & Jakoby, W. B. (1981). Assays for differentiation of glutathione-S-transferase. Methods in Enzymology, 77, 398–405.CrossRefPubMed
18.
King, J. (1965). Isocitrate dehydrogenase. In J. C. King & D. Van (Eds.), Practical clinical enzymology (p. 363). London: Nostrand Co.
19.
Mehler, A. H., Kornberg, A., Grisolia, S., & Ochoa, S. (1948). The enzymatic mechanims of oxidation-reductions between malate or isocitrate or pyruvate. Journal of Biological Chemistry, 174, 961–977.PubMed
20.
Reed, L. J., & Mukherjee, R. B. (1969). Alpha ketoglutarate dehydrogenase complex from Escherichia coli. In J. M. Lowenstein (Ed.), Methods in enzymology (pp. 53–61). London: Academic.
21.
Slater, E. C., & Jr Borner, W.D. (1952). The effect of fluoride on the succinic oxidase system. Biochemistry Journal, 52, 185–196.CrossRef
22.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin’s—Phenol reagent. Journal of Biological Chemistry, 193, 265–275.PubMed
23.
Benzie, F. F., & Strain, J. J. (1999). Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299, 15–23.CrossRefPubMed
24.
Sangeetha, T., & Darlin Quine, S. (2008). Protective effect of S-allyl cysteine sulphoxide (alliin) on glycoproteins and hematology in isoproterenol induced myocardial infarction in male Wistar rats. Journal of Applied Toxicology, 28, 710–716.CrossRefPubMed
25.
Stanely Mainzen Prince, P., Dhanasekar, K., & Rajkumar, S. (2011). Preventive effects of vanillic acid on lipids, Bax, Bcl-2 and myocardial infarct size on isoproterenol-induced myocardial infarcted rats: A biochemical and in vitro study. Cardiovascular Toxicology, 11, 58–66.CrossRefPubMed
26.
Thomes, P., Rajendran, M., Pasanban, B., & Rengasamy, R. (2010). Cardioprotective activity of Cladosiphon okamuranus fucoidan against isoproterenol induced myocardial infarction in rats. International Journal of Phytotherapy and Phytopharmacology, 18, 52–57.CrossRef
27.
Punithavathi, V. R., & Stanely Mainzen Prince, P. (2010). Pretreatment with a combination of quercetin and α-tocopherol ameliorates adenosine triphosphatases and lysosomal enzymes in myocardial infarcted rats. Life Sciences, 86, 178–184.CrossRefPubMed
28.
Saeed, S. A., & Ahmed, S. (2006). Role of cyclooxygenase-2 in myocardial infarction and ischemia. Journal of the College of Physicians and Surgeons Pakistan, 16, 324–328.PubMed
29.
Karthikeyan, K., Sarala Bai, B. R., & Niranjali Devaraj, S. (2007). Grape seed proanthocyanidins ameliorates isoproterenol-induced myocardial injury in rats by stabilizing mitochondrial and lysosomal enzymes: An in vivo study. Life Sciences, 81, 1615–1621.CrossRefPubMed
30.
Zhou, R., Xu, Q., Zheng, P., Yan, L., Zheng, J., & Dai, G. (2008). Cardioprotective effect of fluvastatin on isoproterenol-induced myocardial infarction in rat. European Journal of Pharmacology, 586, 244–250.CrossRefPubMed
31.
Patel, V., Upaganlawar, A., Zalawadia, R., & Balaraman, R. (2010). Cardioprotective effect of melatonin against isoproterenol induced myocardial infarction in rats: A biochemical, electrocardiographic and histoarchitectural evaluation. European Journal of Pharmacology, 644, 160–168.CrossRefPubMed
32.
Stanely Mainzen Prince, P. (2013). (–) Epicatechin prevents alterations in lysosomal glycohydrolases, cathepsins and reduces myocardial infarct size in isoproterenol-induced myocardial infarcted rats. European Journal of Pharmacology, 706, 63–69.CrossRef
33.
Halliwell, B., & Gutteridge, J. M. (1990). Role of free radicals and catalytic metal ions in human disease: An overview. Methods in Enzymology, 18, 61–85.
34.
Devika, P. T., & Stanely Mainzen Prince, P. (2008). (–) Epigallocatechin-gallate (EGCG) prevents mitochondrial damage in isoproterenol-induced cardiac toxicity in albino Wistar rats: A transmission electron microscopic and in vitro study. Pharmacological Research, 57, 351–357.CrossRefPubMed
35.
Hayes, J. D., & Pulford, D. J. (1995). The glutathione-S-transferase supergene family: Regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Critical Reviews in Biochemistry and Molecular Biology, 30, 445–600.CrossRefPubMed
36.
Sangeetha, T., & Darlin Quine, S. (2009). Preventive effect of S-allyl cysteine sulphoxide (Alliin) on mitochondrial dysfunction in normal and isoproterenol induced cardio toxicity in male Wistar rats: A histopathological study. Molecular and Cellular Biochemistry, 328, 1–8.CrossRefPubMed
37.
Stanely Mainzen Prince, P. (2013). (–) Epicatechin attenuates mitochondrial damage by enhancing mitochondrial multi-marker enzymes, adenosine triphosphate and lowering calcium in isoproterenol induced myocardial infarcted rats. Food and Chemical Toxicology, 53, 409–416.CrossRefPubMed
38.
Ma, Y. L., & Ma, C. (2007). Studies on pharmacokinetics of diosmin in rats. Zhongguo Zhongyao Zazhi, 32, 418–420.PubMed
Metadaten
Titel
Diosmin Prevents Isoproterenol-Induced Heart Mitochondrial Oxidative Stress in Rats
verfasst von
S. Sharmila Queenthy
P. Stanely Mainzen Prince
Babu John
Publikationsdatum
17.08.2017
Verlag
Springer US
Erschienen in
Cardiovascular Toxicology / Ausgabe 2/2018
Print ISSN: 1530-7905
Elektronische ISSN: 1559-0259
DOI
https://doi.org/10.1007/s12012-017-9422-2