Background
Methods
Human right atrial myocardium
ITEMS | Healthy controls | NYHA II | NYHA III | P |
---|---|---|---|---|
Male: Female (cases) | 4 : 0 | 8 : 21 | 19 : 25 | N/A |
Age (years) | 44.3 ± 6.70 | 45.8 ± 10.0 | 47.7 ± 9.77 | > 0.05 |
Left atrial dimensions (mm) | N/A | 53.7 ± 11.2 | 58.4 ± 13.2 | > 0.05 |
Mitral Valve involvement (N/%) | N/A | 29 (100%) | 44 (100%) | N/A |
Aortic valve involvement (N/%) | N/A | 9 (31.0%) | 20 (45.5%) | N/A |
Stenosis grade | N/A | 2.10 ± 0.72 | 2.52 ± 0.66 | 0.015* |
RNA isolation and real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR)
Gene | Primer Sequence |
---|---|
ICAM-1/F | CCCCGTTGCCTAAAAAGG |
ICAM-1/R | TTGCACATTGCTCAGTTCATACAC |
DNMT1/F | AAGAACGGCATCCTGTACCGAGTT |
DNMT1/R | TGCTGCCTTTGATGTAGTCGGAGT |
DNMT3a/F | TTTGAGTTCTACCGCCTCCTGCAT |
DNMT3a/R | GTGCAGCTGACACTTCTTTGGCAT |
DNMT3b/F | AGTGTGTGAGGAGTCCATTGCTGT |
DNMT3b/R | GCTTCCGCCAATCACCAAGTCAAA |
β-actin/F | GCACCACACCTTCTACAATGAGC |
β-actin/R | GGATAGCACAGCCTGGATAGCAAC |
Genomic DNA extraction and DNA methylation sequencing
Gene | Primer Sequence |
---|---|
ICAM-1/F | TTAGGGGGAGGGGTATTTTTTAGTG |
ICAM-1/R | TCGCTAACCGCTTCAACTCCGAAAT |