Background
Methods
Study design
Identification of isolates and phage-typing
Susceptibility to antimicrobial agents
Detection of SGI1
Statistical analysis
-
Univariate analysis:
-
Multivariate analysis:
Results
Proportion and characteristics of SGI1-positive nontyphoidal S. Enterica
Serovars | SGI1-positive n (%) | SGI1-negative n (%) |
---|---|---|
S. Typhimurium (n = 36) | 7 (19.4) | 29 (80.6) |
DT104 (n = 9) | 5 (55.6) | 4 (44.4) |
Non DT104 (n = 27) | 2 (7.4) | 25 (92.6) |
Non S. Typhimurium serovars (n = 64) | 5 (7.8) | 59 (92.2) |
0 | 19 (100) | |
S. Enteritidis (n = 14) | 0 | 14 (100) |
S. Paratyphi B var. Java (n = 2) | 0 | 2 (100) |
S. Dublin (n = 5) | 0 | 5 (100) |
Other serovarsa (n = 24) | 5b (20.8) | 19 (79.2) |
Phenotypesc
| SGI1-positive n (%) | SGI1-negative n (%) |
Pansusceptible (n = 21) | 0 (0) | 21 (100) |
Resistant ≥1 (n = 79) | 12 (15.2) | 67 (84.8) |
Including penta-resistant (ACSSuT)d (n = 9) | 6 (67) | 3 (33) |
Variables | SGI1-positive | SGI1-negative | p-valuec
|
---|---|---|---|
N | 12 | 88 | |
Age (years) | 13.9 (56.9) | 18.4 (54.2) |
p = 0.62 |
Sex ratio M/F | 5/7 | 42/46 |
p = 0.77 |
Immunosuppression | 2 (16.7) | 15 (17.0) |
p = 1.00 |
Invasive infection | 2 (16.7) | 13 (14.8) |
p = 1.00 |
Bacteremia | 2 (16.7) | 10 (11.4) |
p = 0.63 |
Sepsis | 2 (16.7) | 13 (14.8) |
p = 1.00 |
Fever or hypothermia | 5 (41.7) | 53 (63.9) |
p = 0.21 |
Missingb
| 0 | 5 | |
Inflammatory response | 11 (100) | 71 (96.0) |
p = 1.00 |
Missingb
| 1 | 14 | |
Bloody stool | 2 (16.7) | 20 (23.5) |
p = 0.73 |
Missingb
| 0 | 3 | |
Leukocytes in stool | 2 (16.7) | 36 (43.9) |
p = 0.11 |
Missingb
| 0 | 6 | |
Intravenous hydration required | 5 (45.5) | 41 (65.1) |
p = 0.31 |
Missingb
| 1 | 25 | |
Length of hospital stay in days | 2.5 (5) | 4 (6) |
p = 0.39 |
Infection with Serovar Typhimurium | 7 (58.3) | 29 (33.0) |
p = 0.11 |
DT104 phage type | 5 (41.6) | 4 (4.5) |
p = 0.001
|
Infection with S. enterica harboring at least 1 resistance | 12 (100) | 67 (76.1) |
p = 0.07 |
Infection with S. enterica harboring penta – resistance (ACSSuT)d
| 6 (50) | 3 (3.4) |
p < 0.001
|
SGI1 and severity of the illness
Impact of antimicrobial resistance on the severity of illness
Antibiotics | Percentage of resistance |
---|---|
Amoxicillin | 37 |
Amoxicillin –clavulanic acid | 17 |
Cefotaxime | 2 |
Nalidixic acid | 9 |
Ofloxacin | 9 |
Kanamycin | 3 |
Streptomycin | 49 |
Spectinomycin | 13 |
Sulphonamides | 52 |
Trimethoprim | 9 |
Chloramphenicol | 12 |
Doxycycline | 61 |
Variables | Infection with pansusceptible isolatea (n = 21) | Infection with isolate harboring at least 1 resistancea (n = 79) | Univariate analysis p-valueb
|
---|---|---|---|
Age (years) | 22.3 (53.7) | 15.8 (54) | 0.26 |
Sex ratio (H/F) | 12/9 | 35/44 | 0.28 |
Immunosuppression | 1 (4.8) | 16 (20.3) | 0.11 |
Invasive infection | 6 (28.6) | 9 (11.4) | 0.08 |
Bacteremia | 6 (28.6) | 6 (7.6) |
0.02
|
Sepsis | 6 (28.6) | 9 (11.4) | 0.08 |
Inflammatory response | 19 (100) | 63 (95.5) | 1.00 |
Missingc
| 2 | 13 | |
Fever or hypothermia | 13 (65) | 45 (60) | 0.80 |
Missingc
| 1 | 4 |
Risk factors to develop nontyphoidal S. enterica invasive infections
Variables (n: number of patients) | Median agea
| iqra
|
p-valueb
|
---|---|---|---|
Invasive infection | |||
No (n = 85) | 12.7 | 53.5 |
p = 0.03
|
Yes (n = 15) | 55.6 | 61.4 | |
Bacteremia | |||
No (n = 88) | 15.5 | 53.6 |
p = 0.08 |
Yes (n = 12) | 43.1 | 64.6 | |
Sepsis | |||
No (n = 85) | 12.7 | 48.8 |
p = 0.01
|
Yes (n = 15) | 57.1 | 46.1 | |
Fever or hypothermia | |||
No (n = 37) | 29 | 58 |
p = 0.24 |
Yes (n = 58) | 9.1 | 51.7 | |
Inflammatory response | |||
No (n = 3) | 2.6 | 1.7 |
p = 0.04
|
Yes (n = 82) | 24.3 | 56.6 |
Variables | Immmunosuppressiona
|
p-valuec
| |
---|---|---|---|
Yes (n = 17) | No (n = 83) | ||
Age (years) | 54.2 (35.7) | 9.7 (54.4) |
p = 0.02
|
Invasive infection | 7 (41.2) | 8 (9.6) |
p = 0.003
|
Bacteremia | 5 (29.4) | 7 (8.4) |
p = 0.03
|
Sepsis | 6 (35.3) | 9 (10.8) |
p = 0.02
|
Fever or hypothermia | 7 (50.0) | 51 (63.0) |
p = 0.39 |
Missingb
| 3 | 2 | |
Inflammatory response | 16 (100) | 66 (95.7) |
p = 1.00 |
Missingb
| 1 | 14 |