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Clinical and Translational Oncology

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01.08.2014 | Research Article

Double-strand breaks on F98 glioma rat cells induced by minibeam and broad-beam synchrotron radiation therapy

verfasst von: S. Gil, Y. Prezado, M. Sabés

Erschienen in: Clinical and Translational Oncology | Ausgabe 8/2014

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Abstract

Purpose

To assess the DNA damage induced by MBRT and BB radiations on glioma cells.

Methods

The analysis of fluorescent intensity emitted per nucleus was plotted versus DNA content 2 and 17 h after irradiations. At around cell-doubling time (17 h) after exposures, the remaining DNA radiation damage could be correlated with cellular death.

Results

A higher γH2AX IF intensity per cell could be detected 2 and 17 h after MBRT when compared with BB. 17 h after MBRT, misrepaired damaged cells remained arrested in both G₁ and G₂ phases.

Conclusions

A pronounced G₂ phase arrest was detected at 17 h after MBRT and BB. However, only after MBRT, a dose-dependent increasing number of damaged cells appeared arrested also in the G₁ phase, and a higher amount of cells more prone to undergo apoptosis were detected. The threshold dose required to enhance the effectiveness of both synchrotron radiation techniques was 12 Gy.

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Titel: Double-strand breaks on F98 glioma rat cells induced by minibeam and broad-beam synchrotron radiation therapy
verfasst von: S. Gil
Y. Prezado
M. Sabés
Publikationsdatum: 01.08.2014
Verlag: Springer Milan
Erschienen in: Clinical and Translational Oncology / Ausgabe 8/2014
Print ISSN: 1699-048X
Elektronische ISSN: 1699-3055
DOI: https://doi.org/10.1007/s12094-013-1134-x

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Double-strand breaks on F98 glioma rat cells induced by minibeam and broad-beam synchrotron radiation therapy

Abstract

Purpose

Methods

Results

Conclusions

Neu im Fachgebiet Onkologie

Prostatakarzinom: EU initiiert neues Screeningkonzept

Stereotaktische Radiatio schlägt konventionelle Bestrahlung

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HNO-Op. auch mit über 90?

Update Onkologie

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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