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01.12.2017 | Short Report | Ausgabe 1/2017 Open Access

Virology Journal 1/2017

Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein

Zeitschrift:
Virology Journal > Ausgabe 1/2017
Autoren:
Xinghong Gao, Renyong Jia, Mingshu Wang, Qiao Yang, Shun Chen, Mafeng Liu, Zhongqiong Yin, Anchun Cheng
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Electronic supplementary material

The online version of this article (doi:10.​1186/​s12985-017-0830-5) contains supplementary material, which is available to authorized users.

Abstract

Background

UL24 is a multifunctional protein that is conserved among alphaherpesviruses and is believed to play an important role in viral infection and replication.

Results

In this paper, to investigate putative UL24-binding proteins and to explore the functional mechanisms of DEV UL24, yeast two-hybrid (Y2H) was carried out, and further verified the interaction between UL24 and partners by co-immunoprecipitation and fluorescence microscopy experiments. Interaction partners of UL24 protein were screened by yeast two-hybrid (Y2H) with the cDNA library of DEV-CHv strain post-infection DEF cells. A novel partner, DEV UL54 protein, was discovered by Y2H screening and bioinformatic. Co-immunoprecipitation experiments suggested that DEV UL24 interacted with UL54 proteins. And distribution of a part of UL54 protein was changed from nucleus to cytoplasm in DF-1 cells of co-subcellular localization experiments which also showed that DEV UL24 interacted with UL54 proteins.

Conclusions

The interaction between the DEV UL24 and UL54 proteins was discovered for the first time. Thus, DEV UL54 protein as a novel partner interacted with DEV UL24 protein.
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