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20.06.2018 | Urology - Original Paper

Dynamic assessment of human sperm DNA damage I: the effect of seminal plasma-sperm co-incubation after ejaculation

verfasst von: Eva Tvrdá, Francisca Arroyo, Jaime Gosálvez

Erschienen in: International Urology and Nephrology | Ausgabe 8/2018

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Abstract

Aim

The purpose of the study was to evaluate the impact of seminal plasma in human ejaculates on the sperm DNA quality and DNA longevity.

Methods

Semen samples for this study were obtained from 20 donors with a normal spermiogram. Following centrifugation, the sperm pellet was resuspended either in the seminal plasma proceeding from its respective donor, or in an equal amount of PBS, adjusting the concentration to 50 × 10⁶/ml. Each set of samples was incubated at 37 °C for 24 h and the sperm DNA damage was assessed using the chromatin-dispersion test following 0, 2, 6, and 24 h of incubation.

Results

Sperm DNA fragmentation (SDF) did not differ between the two experimental conditions at T0; however, Kaplan–Meier estimates to test for survival showed an statistical significant increase of SDF in the seminal plasma group when compared to the PBS group following all timeframes (p 0.000). With respect to sperm DNA longevity, the most dramatic loss of sperm DNA quality occurred during the first 2 h of incubation, with the rate of SDF (rSDF) in the seminal plasma being 2.1 more intense than in PBS. Interestingly, the rSDF was found to vary significantly between individuals, which was confirmed with significant correlations in all rSDF timeframes (rSDF T0–2, p 0.049; rSDF T2–6, p 0.000; rSDF T6–24: p 0.000).

Conclusions

Co-incubation of semen samples in seminal plasma after ejaculation increases iatrogenic sperm damage. This effect is statistically significant after 2 h of co-incubation. Subsequently, for ART purposes seminal plasma must be quickly removed after ejaculation–liquefaction, to avoid a higher susceptibility of sperm DNA towards fragmentation.

Ajduk A, Zernicka-Goetz M (2013) Quality control of embryo development. Mol Asp Med 34(5):903–918. https://doi.org/10.1016/j.mam.2013.03.001 CrossRef

Simon L, Murphy K, Shamsi MB, Liu L, Emery B, Aston KI, Hotaling J, Carrell DT (2014) Paternal influence of sperm DNA integrity on early embryonic development. Hum Reprod 29(11):2402–2412. https://doi.org/10.1093/humrep/deu228 CrossRefPubMed

Alvarez Sedó C, Bilinski M, Lorenzi D, Uriondo H, Noblía F, Longobucco V, Lagar EV, Nodar F (2017) Effect of sperm DNA fragmentation on embryo development: clinical and biological aspects. JBRA Assist Reprod 21(4):343–350. https://doi.org/10.5935/1518-0557.20170061 PubMedCrossRef

Bhattacharya SM (2008) Association of various sperm parameters with unexplained repeated early pregnancy loss—which is most important? Int Urol Nephrol 40(2):391–395CrossRefPubMed

Gosálvez J, Johnston S, López-Fernández C, Gosálbez A, Arroyo F, Fernández JL, Álvarez JG (2014) Sperm fractions obtained following density gradient centrifugation in human ejaculates show differences in sperm DNA longevity. Asian Pac J Reprod 3(2):116–120. https://doi.org/10.1016/S2305-0500(14)60014-2 CrossRef

Esteves SC, Sharma RK, Gosálvez J, Agarwal A (2014) A translational medicine appraisal of specialized andrology testing in unexplained male infertility. Int Urol Nephrol 46(6):1037–1052. https://doi.org/10.1007/s11255-014-0715-0 CrossRefPubMed

López G, Lafuente R, Checa MA, Carreras R, Brassesco M (2013) Diagnostic value of sperm DNA fragmentation and sperm high-magnification for predicting outcome of assisted reproduction treatment. Asian J Androl 15(6):790–794. https://doi.org/10.1038/aja.2013.81 CrossRefPubMedPubMedCentral

Maxwell WM, de Graaf SP, Ghaoui Rel-H, Evans G (2007) Seminal plasma effects on sperm handling and female fertility. Soc Reprod Fertil Suppl 64:13–38PubMed

Boitrelle F, Albert M, Theillac C, Ferfouri F, Bergere M, Vialard F, Wainer R, Bailly M, Selva J (2012) Cryopreservation of human spermatozoa decreases the number of motile normal spermatozoa, induces nuclear vacuolization and chromatin decondensation. J Androl 33(6):1371–1378. https://doi.org/10.2164/jandrol.112.016980 CrossRefPubMed

10.

Martínez-Soto JC, Landeras J, Gadea J (2013) Spermatozoa and seminal plasma fatty acids as predictors of cryopreservation success. Andrology 1(3):365–375. https://doi.org/10.1111/j.2047-2927.2012.00040.x CrossRefPubMed

11.

Fabozzi G, Starita MF, Rega E, Alteri A, Colicchia A, Piscitelli C, Giannini P (2016) Evaluation of the efficiency of two different freezing media and two different protocols to preserve human spermatozoa from cryoinjury. Int J Reprod Med 2016:6059757. https://doi.org/10.1155/2016/6059757 CrossRefPubMedPubMedCentral

12.

Tvrdá E, López-Fernández C, Sánchez-Martín P, Gosálvez J (2018) Sperm DNA fragmentation in donors and normozoospermic patients attending for a first spermiogram: static and dynamic assessment. Andrologia. https://doi.org/10.1111/and.12986 PubMedCrossRef

13.

Bedford JM (2015) The functions—or not—of seminal plasma? Biol Reprod 92(1):1–3. https://doi.org/10.1095/biolreprod.114.126045 CrossRef

14.

Crawford G, Ray A, Gudi A, Shah A, Homburg R (2015) The role of seminal plasma for improved outcomes during in vitro fertilization treatment: review of the literature and meta-analysis. Hum Reprod Update 21(2):275–284. https://doi.org/10.1093/humupd/dmu052 CrossRefPubMed

15.

de la Torre J, Sánchez-Martín P, Gosálvez J, Crespo F (2017) Equivalent seminal characteristics in human and stallion at first and second ejaculated fractions. Andrologia 49(8):e12708. https://doi.org/10.1111/and.12708 CrossRef

16.

Intasqui P, Antoniassi MP, Camargo M, Nichi M, Carvalho VM, Cardozo KH, Zylbersztejn DS, Bertolla RP (2015) Differences in the seminal plasma proteome are associated with oxidative stress levels in men with normal semen parameters. Fertil Steril 104(2):292–301. https://doi.org/10.1016/j.fertnstert.2015.04.037 CrossRefPubMed

17.

Guz J, Gackowski D, Foksinski M, Rozalski R, Zarakowska E, Siomek A, Szpila A, Kotzbach M, Kotzbach R, Olinski R (2013) Comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men. PLoS ONE 8(7):e68490. https://doi.org/10.1371/journal.pone.0068490 CrossRefPubMedPubMedCentral

18.

Hosen MB, Islam MR, Begum F, Kabir Y, Howlader MZ (2015) Oxidative stress induced sperm DNA damage, a possible reason for male infertility. Iran J Reprod Med 13(9):525–532PubMedPubMedCentral

19.

Yamauchi Y, Ajduk A, Riel JM, Ward MA (2007) Ejaculated and epididymal mouse spermatozoa are different in their susceptibility to nuclease-dependent DNA damage and in their nuclease activity. Biol Reprod 77:636–647CrossRefPubMed

20.

Fernandez-Encinas A, García-Peiró A, Ribas-Maynou J, Abad C, Amengual MJ, Navarro J, Benet J (2016) Characterization of nuclease activity in human seminal plasma and its relationship to semen parameters, sperm DNA fragmentation and male infertility. J Urol 195(1):213–219. https://doi.org/10.1016/j.juro.2015.07.089 CrossRefPubMed

21.

Cooper TG, Noonan E, von Eckardstein S, Auger J, Baker HW, Behre HM, Haugen TB, Kruger T, Wang C, Mbizvo MT, Vogelsong KM (2010) World Health Organization reference values for human semen characteristics. Hum Reprod Update 16:231–245. https://doi.org/10.1093/humupd/dmp048 CrossRefPubMed

22.

Gosálvez J, Caballero P, López-Fernández C, Ortega L, Guijarro JA, Fernández JL, Johnston SD, Nuñez-Calonge R (2013) Can DNA fragmentation of neat or swim-up spermatozoa be used to predict pregnancy following ICSI of fertile oocyte donors? Asian J Androl 15(6):812–818. https://doi.org/10.1038/aja.2013.74 CrossRefPubMedPubMedCentral

23.

Zhao J, Zhang Q, Wang Y, Li Y (2014) Whether sperm deoxyribonucleic acid fragmentation has an effect on pregnancy and miscarriage after in vitro fertilization/intracytoplasmic sperm injection: a systematic review and meta-analysis. Fertil Steril 102(4):998–1005.e8. https://doi.org/10.1016/j.fertnstert.2014.06.033 CrossRefPubMed

24.

Zhang Z, Zhu L, Jiang H, Chen H, Chen Y, Dai Y (2015) Sperm DNA fragmentation index and pregnancy outcome after IVF or ICSI: a meta-analysis. J Assist Reprod Genet 32(1):17–26. https://doi.org/10.1007/s10815-014-0374-1 CrossRefPubMed

25.

Kussler APS, Pimentel AM, Alcoba DD, Liu IP, Brum IS, Capp E, Corleta HVE (2014) Mechanical processing of hyperviscous semen specimens can negatively affect sperm DNA fragmentation. Int Urol Nephrol 46(4):737–742. https://doi.org/10.1007/s11255-013-0578-9 CrossRefPubMed

26.

Esteves SC, Gosálvez J, López-Fernández C, Núñez-Calonge R, Caballero P, Agarwal A, Fernández JL (2015) Diagnostic accuracy of sperm DNA degradation index (DDSi) as a potential noninvasive biomarker to identify men with varicocele-associated infertility. Int Urol Nephrol 47(9):1471–1477. https://doi.org/10.1007/s11255-015-1053-6 CrossRefPubMed

27.

Ben WX, Fu MT, Mao LK, Ming ZW, Xiong WW (1997) Effects of various concentrations of native seminal plasma in cryoprotectant on viability of human sperm. Arch Androl 39(3):211–216CrossRefPubMed

28.

Kobayashi M, Hori T, Kawakami E (2013) Changes in prostaglandin E2 levels in seminal plasma during ejaculation and the effect of exogenous prostaglandin E2 on semen volume in the dog. J Vet Med Sci 75(9):1249–1252. https://doi.org/10.1292/jvms.13-0053 CrossRefPubMed

29.

Akcay E, Reilas T, Andersson M, Katila T (2006) Effect of seminal plasma fractions on stallion sperm survival after cooled storage. J Vet Med A 53(9):481–485. https://doi.org/10.1111/j.1439-0442.2006.00882.x CrossRef

30.

Benchaib M, Braun V, Lornage J, Hadj S, Salle B, Lejeune H, Guérin JF (2003) Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum Reprod 18(5):1023–1028. https://doi.org/10.1093/humrep/deg228 CrossRefPubMed

31.

Manjunath P, Bergeron A, Lefebvre J, Fan J (2007) Seminal plasma proteins: functions and interaction with protective agents during semen preservation. Soc Reprod Fertil Suppl 65:217–228PubMed

32.

Brachtchenko J, Moskovstev S, Swanson S, Shlush E, Kan-Ool L, Sharma P, Baratz AY, Glass K, Librach CL (2015) Significant correlation between sperm DNA damage and achieving a pregnancy in 1847 non-IVF fertility treatment cycles. Fertil Steril 104(3):e297CrossRef

33.

Wdowiak A, Bojar I (2016) Relationship between pregnancy, embryo development, and sperm deoxyribonucleic acid fragmentation dynamics. Saudi J Biol Sci 23(5):598–606. https://doi.org/10.1016/j.sjbs.2015.08.001 CrossRefPubMed

34.

Har-Vardi I, Mali R, Breietman M, Sonin Y, Albotiano S, Levitas E, Potashnik G, Priel E (2007) DNA topoisomerases I and II in human mature sperm cells: characterization and unique properties. Hum Reprod 22(8):2183–2189. https://doi.org/10.1093/humrep/dem170 CrossRefPubMed

35.

Har-Vardi I, Breitman M, Potashnik G, Priel E (1999) Characterization of DNA topoisomerases in human sperm cells. Hum Reprod 14(Suppl 3):33. https://doi.org/10.1093/humrep/14.Suppl_3.33 CrossRef

36.

Shaman JA, Prisztoka R, Ward WS (2006) Topoisomerase IIB and an extracellular nuclease interact to digest sperm DNA in an apoptotic-like manner. Biol Reprod 75(5):741–748. https://doi.org/10.1095/biolreprod.106.055178 CrossRefPubMed

37.

Sotolongo B, Huang TT, Isenberger E, Ward WS (2005) An endogenous nuclease in hamster, mouse, and human spermatozoa cleaves DNA into loop-sized fragments. J Androl 26(2):272–280. https://doi.org/10.1002/j.1939-4640.2005.tb01095.x CrossRefPubMed

38.

Knuth UA, Kühne J, Bals-Pratsch M, Nieschlag E (1988) Intra-individual variation of sperm velocity, linearity, lateral head displacement and beat frequency in healthy volunteers. Andrologia 20(3):243–248. https://doi.org/10.1111/j.1439-0272.1988.tb01065.x CrossRefPubMed

39.

Auger J, Eustache F, Ducot B, Blandin T, Daudin M, Diaz I, Matribi SE, Gony B, Keskes L, Kolbezen M, Lamarte A, Lornage J, Nomal N, Pitaval G, Simon O, Virant-Klun I, Spira A, Jouannet P (2000) Intra- and inter-individual variability in human sperm concentration, motility and vitality assessment during a workshop involving ten laboratories. Hum Rep 15(11):2360–2368. https://doi.org/10.1093/humrep/15.11.2360 CrossRef

40.

Dere E, Huse S, Hwang K, Sigman M, Boekelheide K (2016) Intra- and inter-individual differences in human sperm DNA methylation. Andrology 4(5):832–842. https://doi.org/10.1111/andr.12170 CrossRefPubMedPubMedCentral

Titel: Dynamic assessment of human sperm DNA damage I: the effect of seminal plasma-sperm co-incubation after ejaculation
verfasst von: Eva Tvrdá
Francisca Arroyo
Jaime Gosálvez
Publikationsdatum: 20.06.2018
Verlag: Springer Netherlands
Erschienen in: International Urology and Nephrology / Ausgabe 8/2018
Print ISSN: 0301-1623
Elektronische ISSN: 1573-2584
DOI: https://doi.org/10.1007/s11255-018-1915-9

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Dynamic assessment of human sperm DNA damage I: the effect of seminal plasma-sperm co-incubation after ejaculation

Abstract

Aim

Methods

Results

Conclusions

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Conclusions

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Leitlinien kompakt für die Innere Medizin

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Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Metformin rückt in den Hintergrund

Update Innere Medizin