Serum paraoxonase 1 (PON1), an enzyme associated with high – density lipoproteins (HDL) particles, inhibits the oxidation of serum lipoproteins and cell membranes. PON1 activity is lower in patients with atherosclerosis and in inflammatory diseases. The systemic inflammatory response provoked during cardiopulmonary bypass grafting may contribute to the development of postoperative complications. The aim of the present study was to estimate the dynamic changes in paraoxonase 1 (PON1) activity towards paraoxon and phenyl acetate during and after coronary artery surgery.
Twenty six patients with coronary heart disease undergoing coronary artery bypass grafting (CABG) were enrolled into the study. Venous blood samples were obtained preoperatively, after aortic clumping, after the end of operation, at 6, 18, 30 and 48 h after operation. Paraoxonase activity was measured spectrophotometrically in 50 mM glycine/NaOH buffer (pH 10.5) containing 1.0 mM paraoxon, and 1.0 mM CaCl2. Arylesterase activity was measured in 20 mM TrisCl buffer (pH 8.0) containing 1 mM phenyl acetate and 1 mM CaCl2.
PON1 activity toward paraoxon and phenyl acetate significantly decreased after aorta cross clumping and increased directly after operation. PON1 activity towards paraoxon in preoperative period and PON1 activity towards phenyl acetate in seventh stage of experiment tended to inversely correlate with the occurrence of postoperative complications.
The paraoxonase 1 plasma activity is markedly reduced during CABG surgery.
Mackness B, Quarck R, Verreth W, et al. Human paraoxonase −1 overexpression inhibits atherosclerosis in a mouse model of a metabolic syndrome. Arteriosc Thromb Vasc Biol. 2006;26:1545–50. CrossRef
Durmaz T, Keles T, Ayhan H, et al. Diminished serum paraoxonase activity in patients with coronary artery calcification. Kardiol Pol. 2014;72:831–838.
van Himbergen TM, van der Schouw YT, Voorbij HAM, et al. Paraoxonase1 (PON1) and the risk for coronary heart disease and myocardial infarction in a general population of Dutch women. Atherosclerosis. 2008;198:408–14. CrossRef
Paparella D, Yau TM, Young E. Cardiopulmonary bypass induced inflammation: pathophysiology and treatment. An update. Eur J Cardio- th Surg. 2002;21:232–44. CrossRef
LaDu BN. Human serum paraoxonase/arylesterase. In: Kalow W, editor. Pharmacogenetics of drug metabolism. New York: Pergamon Press; 1992. p. 51–91.
Aviram M, Billecke S, Sorenson R, et al. Paraoxonase active site is required for protection against LDL oxidation involves its free sulfhydryl group and is different from that required for its arylesterase/paraoxonase activities: selective action of human paraoxonase Q and R. Arterioscer Thromb Vasc Biol. 1998;18:1617–24. CrossRef
Sanghera DK, Saha N, Aston CE, et al. DNA polymorphism in two paraoxonase genes (PON1 and PON2) are associated with the risk od coronary heart disease. Am J Hum Genet. 1998;314:410–8.
Anitkainen M, Muromtki S, Syvnne M, et al. The Gln–Arg 192 polymorphism of the human paraoxonase gene is not associated with the risk of coronary heart disease in Finns. J Clin Invest. 1996;191:883–5. CrossRef
Mackness B, Turkie W, Mackness M. Paraoxonase −1 (PON1) promoter region polymorphisms, serum PON1 status and coronary heart disease. Arch Med Sci. 2013;1:8–13. CrossRef
Kunt AS, Selek S, Celik H, et al. Decrease of total antioxidant capacity during coronary artery bypass surgery. Mt Sinai J Med. 2006;73:777–83. PubMed
Hatemi AC, Ceviker K, Togut A, et al. Oxidant status following cardiac surgery with phosphorylcholine-coated extracorporeal circulation systems. Oxidative Med Cell Longev. 2016;2016:3932092. CrossRef
Das, Vasisht Nm Das L et al: Correlation between total antioxidant staus and lipid peroxidation in hypercholesterolemia. Current Science. 2000;78:486–487,
Maxkness B, Hunt R, Durrington PN. Mackness MI: ncreased immunolocalization of paraoxonase, clusterin, and apolipoprotein A-I in the human artery wall with the progression of atherosclerosis. Arterioscler Thromb Vasc Biol. 1997;17:1233–8. CrossRef
Arrol S, Mackness MI, Durrington PN. High-density lipoprotein associated enzymes and the prevention of low-density lipoprotein oxidation. Eur J Lab Med. 1996;4:33–8.
Sato M, Ohkawa R, Yoshimoto A. Effects of serum amyloid a on the structure and antioxidant ability of high density lipoprotein. Biosci Rep. 2016;36 doi: 10.1042/BRS20160075.
Kunnen S, Van Eck M. Lecithin: cholesterol acyltransferase: old friend or foe in atheroclerosis? J Lipid Res. 2012;53(1783):1799.
Kumon Y, Nakauchi Y, Kidawara K, et al. A longitudinal analysis of alteration in lecithin-cholesterol acyltransferase and paraoxonase activities following laparoscopic cholecystectomy relative to other parameters of HDL function and the acute phase response. Scand J Immunol. 1998;48:419–24. CrossRefPubMed
WenJun D. Qiang Ji, YunQing Shi et al: predictors of low cardiac out put syndrome after isolated coronary artery bypass grafting. Int Heart J. 2015;56:144–9. CrossRef
- Dynamic changes of paraoxonase 1 activity towards paroxon and phenyl acetate during coronary artery surgery
- BioMed Central
Neu im Fachgebiet Kardiologie
Mail Icon II