E-cadherin and periostin in early detection and progression of diabetic nephropathy: epithelial-to-mesenchymal transition
- 18.05.2019
- Original article
Erschienen in:
- Verfasst von
- Nada M. Qamar El-Dawla
- Al-Aliaa M. Sallam
- Mohamed H. El-Hefnawy
- Hala O. El-Mesallamy
- Erschienen in
- Clinical and Experimental Nephrology | Ausgabe 8/2019
Abstract
Background
Diabetic nephropathy (DN) is a severe complication of diabetes mellitus (DM). Many mechanisms are involved in its development; one of these mechanisms is epithelial-to-mesenchymal transition (EMT). During EMT, losing of the epithelial biomarkers like E-cadherin and increasing of mesenchymal biomarkers like periostin are very characteristic.
Methods
The study included 19 healthy controls and 71 DN patients categorized according to their urinary albumin-to-creatinine ratio (UACR) into 19 normoalbuminuric (UACR < 30 mg/g), 37 microalbuminuric (UACR 30–300 mg/g), and 15 macroalbuminuric (UACR > 300 mg/g) patients. Fasting plasma glucose (FPG), glycated hemoglobin (HbA1C%), serum creatinine (Cr), and urea were measured. E-cadherin and periostin were measured by ELISA and compared among groups.
Results
Concerning E-cadherin levels, in comparison to control group, there were significantly decreased in all groups (0.94, 0.52, and 0.14 ng/mL in normoalbuminuria, microalbuminuria, and macroalbuminuria groups; respectively). For periostin levels, nonsignificant increase in normoalbuminuria (0.32 ng/mL) than control group (0.3 ng/mL) was observed. There was a significant increase in other groups with the highest values in macroalbuminuria group (1.66 ng/mL). E-cadherin and periostin were correlated with each other (r = − 0.353, P < 0.001). UACR was negatively correlated with E-cadherin and positively correlated with periostin. ROC curve analyses showed that the AUC to diagnose established microalbuminuria using E-cadherin was 0.998 (95% CI 0. 932–1), and using periostin was 0.833 (95% CI 0.709–0.919).
Conclusion
Serum E-cadherin and periostin could be considered as reliable biomarkers involved in DN pathogenesis and linked to its stages.
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- Titel
- E-cadherin and periostin in early detection and progression of diabetic nephropathy: epithelial-to-mesenchymal transition
- Verfasst von
-
Nada M. Qamar El-Dawla
Al-Aliaa M. Sallam
Mohamed H. El-Hefnawy
Hala O. El-Mesallamy
- Publikationsdatum
- 18.05.2019
- Verlag
- Springer Singapore
- Erschienen in
-
Clinical and Experimental Nephrology / Ausgabe 8/2019
Print ISSN: 1342-1751
Elektronische ISSN: 1437-7799 - DOI
- https://doi.org/10.1007/s10157-019-01744-3
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