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Erschienen in: European Journal of Nutrition 7/2019

03.10.2018 | Original Contribution

Effect of an egg ovalbumin-derived protein hydrolysate on blood pressure and cardiovascular risk in adults with a mildly elevated blood pressure: a randomized placebo-controlled crossover trial

verfasst von: Alice J. Lucey, Clara Heneghan, Edmund Manning, Paul A. Kroon, Máiréad E. Kiely

Erschienen in: European Journal of Nutrition | Ausgabe 7/2019

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Abstract

Purpose

While animal and in vitro data demonstrate vasodilatory effects of egg white-derived peptides, human studies are lacking. We investigated for the first time the effects of an egg ovalbumin-derived protein hydrolysate on blood pressure (BP) and cardiovascular risk.

Methods

A double-blind, placebo-controlled randomized crossover trial was implemented in 75 adults aged 50–70 years with systolic BP (130–≤ 150 mmHg). Participants were randomized to an egg ovalbumin-derived protein hydrolysate (3 g/day) or placebo (3 g/day). Participants completed two 6-week periods separated by a 3-week washout.

Results

Data from 65 participants with a mean systolic BP (135.1 ± 11 mmHg) were included. Mean office and central BP and arterial stiffness (assessed by carotid-femoral pulse wave velocity (cfPWV) or pulse wave analysis (PWA)) did not change over time and no significant differences were observed between the egg protein hydrolysate and placebo groups (P > 0.05). Similarly, no significant effects of this egg ovalbumin-derived protein hydrolysate on blood lipid and glucose concentrations (P > 0.05) were observed.

Conclusion

This is the first dietary intervention to investigate the effects of egg ovalbumin-derived protein hydrolysates on cardiovascular risk in humans. Despite promising findings from animal and in vitro studies, this RCT does not support the hypothesis that consumption of an egg ovalbumin-derived protein hydrolysate for 6 weeks in adults with a high-normal BP results in a reduction in BP or the modification of cardiovascular risk.
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Literatur
1.
Zurück zum Zitat Mozaffarian D, Benjamin EJ, Go AS, Arnett DK et al (2016) Heart disease and stroke statistics—2016 update. Rep Am Heart Assoc 133:e38–e360 Mozaffarian D, Benjamin EJ, Go AS, Arnett DK et al (2016) Heart disease and stroke statistics—2016 update. Rep Am Heart Assoc 133:e38–e360
2.
Zurück zum Zitat Landsberg L, Aronne LJ, Beilin LJ, Burke V et al (2013) Obesity-related hypertension: pathogenesis, cardiovascular risk, and treatment: a position paper of the obesity society and the American society of hypertension. J Clin Hypertens 15:14–33CrossRef Landsberg L, Aronne LJ, Beilin LJ, Burke V et al (2013) Obesity-related hypertension: pathogenesis, cardiovascular risk, and treatment: a position paper of the obesity society and the American society of hypertension. J Clin Hypertens 15:14–33CrossRef
3.
Zurück zum Zitat Appel LJ, Moore TJ, Obarzanek E, Vollmer WM et al (1997) A clinical trial of the effects of dietary patterns on blood pressure. New Engl J Med 336:1117–1124CrossRef Appel LJ, Moore TJ, Obarzanek E, Vollmer WM et al (1997) A clinical trial of the effects of dietary patterns on blood pressure. New Engl J Med 336:1117–1124CrossRef
4.
Zurück zum Zitat Saneei P, Salehi-Abargouei A, Esmaillzadeh A, Azadbakht L (2014) Influence of dietary approaches to stop hypertension (DASH) diet on blood pressure: a systematic review and meta-analysis on randomized controlled trials. Nutr Metab Cardiovas 24:1253–1261CrossRef Saneei P, Salehi-Abargouei A, Esmaillzadeh A, Azadbakht L (2014) Influence of dietary approaches to stop hypertension (DASH) diet on blood pressure: a systematic review and meta-analysis on randomized controlled trials. Nutr Metab Cardiovas 24:1253–1261CrossRef
5.
Zurück zum Zitat Hernández-Ledesma B, Del Mar Contreras M, Recio I (2011) Antihypertensive peptides: Production, bioavailability and incorporation into foods. Adv Colloid Interface Sci 165:23–35CrossRef Hernández-Ledesma B, Del Mar Contreras M, Recio I (2011) Antihypertensive peptides: Production, bioavailability and incorporation into foods. Adv Colloid Interface Sci 165:23–35CrossRef
6.
Zurück zum Zitat Cicero AFG, Fogacci F, Colletti A (2017) Potential role of bioactive peptides in prevention and treatment of chronic diseases: a narrative review. Br J Pharmacol 174:1378–1394CrossRef Cicero AFG, Fogacci F, Colletti A (2017) Potential role of bioactive peptides in prevention and treatment of chronic diseases: a narrative review. Br J Pharmacol 174:1378–1394CrossRef
7.
Zurück zum Zitat Cicero AFG, Gerocarni B, Laghi L, Borghi C (2011) Blood pressure lowering effect of lactotripeptides assumed as functional foods: a meta-analysis of current available clinical trials. J Hum Hypertens 25:425–436CrossRef Cicero AFG, Gerocarni B, Laghi L, Borghi C (2011) Blood pressure lowering effect of lactotripeptides assumed as functional foods: a meta-analysis of current available clinical trials. J Hum Hypertens 25:425–436CrossRef
9.
Zurück zum Zitat Miguel M, Manso MA, Martín-Álvarez PJ, Aleixandre A et al (2007) Angiotensin-converting enzyme activity in plasma and tissues of spontaneously hypertensive rats after the short- and long-term intake of hydrolysed egg white. Mol Nutr Food Res 51:555–563CrossRef Miguel M, Manso MA, Martín-Álvarez PJ, Aleixandre A et al (2007) Angiotensin-converting enzyme activity in plasma and tissues of spontaneously hypertensive rats after the short- and long-term intake of hydrolysed egg white. Mol Nutr Food Res 51:555–563CrossRef
10.
Zurück zum Zitat Miguel M, López-Fandiño R, Ramos M, Aleixandre A (2006) Long-term intake of egg white hydrolysate attenuates the development of hypertension in spontaneously hypertensive rats. Life Sci 78:2960–2966CrossRef Miguel M, López-Fandiño R, Ramos M, Aleixandre A (2006) Long-term intake of egg white hydrolysate attenuates the development of hypertension in spontaneously hypertensive rats. Life Sci 78:2960–2966CrossRef
11.
Zurück zum Zitat Garcia-Redondo AB, Roque FR, Miguel M, López-Fandiño R et al (2011) Vascular effects of egg white-derived peptides in resistance arteries from rats. Structure-activity relationships. J Sci Food Agr 90:1988–1993 Garcia-Redondo AB, Roque FR, Miguel M, López-Fandiño R et al (2011) Vascular effects of egg white-derived peptides in resistance arteries from rats. Structure-activity relationships. J Sci Food Agr 90:1988–1993
12.
Zurück zum Zitat Miguel M, López-Fandiño R, Ramos M, Aleixandre A (2005) Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats. Br J Nutr 94:731–737CrossRef Miguel M, López-Fandiño R, Ramos M, Aleixandre A (2005) Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats. Br J Nutr 94:731–737CrossRef
13.
Zurück zum Zitat Plat J, Severins N, Morrison S, Mensink RP (2017) Effects of NWT-03, an egg-protein hydrolysate, on blood pressure in normotensive and mild hypertensive men and women: a dose-finding study. Br J Nutr 117:942–950CrossRef Plat J, Severins N, Morrison S, Mensink RP (2017) Effects of NWT-03, an egg-protein hydrolysate, on blood pressure in normotensive and mild hypertensive men and women: a dose-finding study. Br J Nutr 117:942–950CrossRef
14.
Zurück zum Zitat Cicero AF, Rosticci M, Veronesi M, Bacchelli S et al (2010) Hemodynamic effects of lactotripeptides from casein hydrolysate in Mediterranean normotensive subjects and patients with high-normal blood pressure: a randomized, double-blind, crossover clinical trial. J Med Food 13:1363–1368CrossRef Cicero AF, Rosticci M, Veronesi M, Bacchelli S et al (2010) Hemodynamic effects of lactotripeptides from casein hydrolysate in Mediterranean normotensive subjects and patients with high-normal blood pressure: a randomized, double-blind, crossover clinical trial. J Med Food 13:1363–1368CrossRef
15.
Zurück zum Zitat Van Mierlo LA, Koning MMG, Van Zander KD, Draijer R (2009) Lactotripeptides do not lower ambulatory blood pressure in untreated whites: results from 2 controlled multicenter crossover studies. Am J Clin Nutr 89:617–623CrossRef Van Mierlo LA, Koning MMG, Van Zander KD, Draijer R (2009) Lactotripeptides do not lower ambulatory blood pressure in untreated whites: results from 2 controlled multicenter crossover studies. Am J Clin Nutr 89:617–623CrossRef
16.
Zurück zum Zitat Miguel M, Recio I, Gomez-Ruiz JA, Ramos M, Lopez-Fandino R (2004) Angiotensin I-converting enzyme inhibitory activity of peptides derived from egg white proteins by enzymatic hydrolysis. J Food Prot 67:1914–1920CrossRef Miguel M, Recio I, Gomez-Ruiz JA, Ramos M, Lopez-Fandino R (2004) Angiotensin I-converting enzyme inhibitory activity of peptides derived from egg white proteins by enzymatic hydrolysis. J Food Prot 67:1914–1920CrossRef
17.
Zurück zum Zitat Davalos A, Miguel M, Bartolome B, Lopez-Fandino R (2004) Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis. J Food Prot 67:1939–1944CrossRef Davalos A, Miguel M, Bartolome B, Lopez-Fandino R (2004) Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis. J Food Prot 67:1939–1944CrossRef
18.
Zurück zum Zitat Grootaert CJG, Matthijs B, Pitart J, Baggerman G et al (2017) Quantification of egg ovalbumin hydrolysate-derived anti-hypertensive peptides in an in vitro model combining luminal digestion with intestinal Caco-2 cell transport. Food Res Int 99:513–541CrossRef Grootaert CJG, Matthijs B, Pitart J, Baggerman G et al (2017) Quantification of egg ovalbumin hydrolysate-derived anti-hypertensive peptides in an in vitro model combining luminal digestion with intestinal Caco-2 cell transport. Food Res Int 99:513–541CrossRef
19.
Zurück zum Zitat Miguel M, Aleixandre A (2006) Antihypertensive peptides derived from egg proteins. J Nutr 136:1457–1460CrossRef Miguel M, Aleixandre A (2006) Antihypertensive peptides derived from egg proteins. J Nutr 136:1457–1460CrossRef
20.
Zurück zum Zitat Miguel M, Manso M, Aleixandre A, Alonso MJ et al (2007) Vascular effects, angiotensin I-converting enzyme (ACE)-inhibitory activity, and antihypertensive properties of peptides derived from egg white. J Agr Food Chem 55:10615–10621CrossRef Miguel M, Manso M, Aleixandre A, Alonso MJ et al (2007) Vascular effects, angiotensin I-converting enzyme (ACE)-inhibitory activity, and antihypertensive properties of peptides derived from egg white. J Agr Food Chem 55:10615–10621CrossRef
21.
Zurück zum Zitat Mancia G, Fagard R, Narkiewicz K, Redon J et al (2014) 2013 ESH/ESC practice guidelines for the management of arterial hypertension. Blood Press 23:3–16CrossRef Mancia G, Fagard R, Narkiewicz K, Redon J et al (2014) 2013 ESH/ESC practice guidelines for the management of arterial hypertension. Blood Press 23:3–16CrossRef
22.
Zurück zum Zitat Laurent S, Marais L, Boutouyrie P (2016) The noninvasive assessment of vascular aging. Can J Cardiol 32:669–679CrossRef Laurent S, Marais L, Boutouyrie P (2016) The noninvasive assessment of vascular aging. Can J Cardiol 32:669–679CrossRef
23.
Zurück zum Zitat Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18:499–502 Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18:499–502
24.
Zurück zum Zitat Cicero AFG, Aubin F, Azais-Braesco V, Borghi C (2013) Do the lactotripeptides isoleucine–proline–proline and valine–proline–proline reduce systolic blood pressure in European subjects? A meta-analysis of randomized controlled trials. Am J Hypertens 26:442–449CrossRef Cicero AFG, Aubin F, Azais-Braesco V, Borghi C (2013) Do the lactotripeptides isoleucine–proline–proline and valine–proline–proline reduce systolic blood pressure in European subjects? A meta-analysis of randomized controlled trials. Am J Hypertens 26:442–449CrossRef
25.
Zurück zum Zitat Fekete ÁA, Givens DI, Lovegrove JA (2015) Casein-derived lactotripeptides reduce systolic and diastolic blood pressure in a meta-analysis of randomised clinical trials. Nutrients 7:659–681CrossRef Fekete ÁA, Givens DI, Lovegrove JA (2015) Casein-derived lactotripeptides reduce systolic and diastolic blood pressure in a meta-analysis of randomised clinical trials. Nutrients 7:659–681CrossRef
26.
Zurück zum Zitat Fekete ÁA, Givens DI, Lovegrove JA (2016) Can milk proteins be a useful tool in the management of cardiometabolic health? An updated review of human intervention trials. Proc Nutr Soc 75:328–341CrossRef Fekete ÁA, Givens DI, Lovegrove JA (2016) Can milk proteins be a useful tool in the management of cardiometabolic health? An updated review of human intervention trials. Proc Nutr Soc 75:328–341CrossRef
27.
Zurück zum Zitat Boelsma E, Kloek J (2010) IPP-rich milk protein hydrolysate lowers blood pressure in subjects with stage 1 hypertension, a randomized controlled trial. Nutr J 9:52CrossRef Boelsma E, Kloek J (2010) IPP-rich milk protein hydrolysate lowers blood pressure in subjects with stage 1 hypertension, a randomized controlled trial. Nutr J 9:52CrossRef
28.
Zurück zum Zitat Cicero AFG, Colletti A, Rosticci M, Cagnati M et al (2016) Effect of lactotripeptides (isoleucine–proline–proline/valine–proline–proline) on blood pressure and arterial stiffness changes in subjects with suboptimal blood pressure control and metabolic syndrome: a double-blind, randomized, crossover clinical trial. Metab Syndr Rel Disord 14:161–166CrossRef Cicero AFG, Colletti A, Rosticci M, Cagnati M et al (2016) Effect of lactotripeptides (isoleucine–proline–proline/valine–proline–proline) on blood pressure and arterial stiffness changes in subjects with suboptimal blood pressure control and metabolic syndrome: a double-blind, randomized, crossover clinical trial. Metab Syndr Rel Disord 14:161–166CrossRef
29.
Zurück zum Zitat Seppo L, Jauhiainen T, Poussa T, Korpela R (2003) A fermented milk high in bioactive peptides has a blood pressure-lowering effect in hypertensive subjects. Am J Clin Nutr 77:326–330CrossRef Seppo L, Jauhiainen T, Poussa T, Korpela R (2003) A fermented milk high in bioactive peptides has a blood pressure-lowering effect in hypertensive subjects. Am J Clin Nutr 77:326–330CrossRef
30.
Zurück zum Zitat Turpeinen AM, Ehlers PI, Kivimäki AS, Järvenpää S et al (2011) Ile–Pro–Pro and Val–Pro–Pro tripeptide-containing milk product has acute blood pressure lowering effects in mildly hypertensive subjects. Clin Exp Hypertens 33:388–396CrossRef Turpeinen AM, Ehlers PI, Kivimäki AS, Järvenpää S et al (2011) Ile–Pro–Pro and Val–Pro–Pro tripeptide-containing milk product has acute blood pressure lowering effects in mildly hypertensive subjects. Clin Exp Hypertens 33:388–396CrossRef
31.
Zurück zum Zitat McEniery CM, Cockcroft JR, Roman MJ, Franklin SS et al (2014) Central blood pressure: current evidence and clinical importance. Eur Heart J 35:1719–1725CrossRef McEniery CM, Cockcroft JR, Roman MJ, Franklin SS et al (2014) Central blood pressure: current evidence and clinical importance. Eur Heart J 35:1719–1725CrossRef
32.
Zurück zum Zitat Roman MJ, Devereux RB, Kizer JR, Okin PM et al (2009) High central pulse pressure is independently associated with adverse cardiovascular outcome: the strong heart study. J Am Coll Cardiol 54:1730–1734CrossRef Roman MJ, Devereux RB, Kizer JR, Okin PM et al (2009) High central pulse pressure is independently associated with adverse cardiovascular outcome: the strong heart study. J Am Coll Cardiol 54:1730–1734CrossRef
33.
Zurück zum Zitat Cicero AFG, Rosticci M, Gerocarni B, Bacchelli S et al (2011) Lactotripeptides effect on office and 24-h ambulatory blood pressure, blood pressure stress response, pulse wave velocity and cardiac output in patients with high-normal blood pressure or first-degree hypertension: a randomized double-blind clinical trial. Hypertens Res 34:1035–1040CrossRef Cicero AFG, Rosticci M, Gerocarni B, Bacchelli S et al (2011) Lactotripeptides effect on office and 24-h ambulatory blood pressure, blood pressure stress response, pulse wave velocity and cardiac output in patients with high-normal blood pressure or first-degree hypertension: a randomized double-blind clinical trial. Hypertens Res 34:1035–1040CrossRef
34.
Zurück zum Zitat Jauhiainen T, Ronnback M, Vapaatalo H, Wuolle K et al (2010) Long-term intervention with Lactobacillus helveticus fermented milk reduces augmentation index in hypertensive subjects. Eur J Clin Nutr 64:424–431CrossRef Jauhiainen T, Ronnback M, Vapaatalo H, Wuolle K et al (2010) Long-term intervention with Lactobacillus helveticus fermented milk reduces augmentation index in hypertensive subjects. Eur J Clin Nutr 64:424–431CrossRef
35.
Zurück zum Zitat Miguel M, Alonso MJ, Salaices M, Aleixandre A et al (2007) Antihypertensive, ACE-inhibitory and vasodilator properties of an egg white hydrolysate: effect of a simulated intestinal digestion. Food Chem 104:163–168CrossRef Miguel M, Alonso MJ, Salaices M, Aleixandre A et al (2007) Antihypertensive, ACE-inhibitory and vasodilator properties of an egg white hydrolysate: effect of a simulated intestinal digestion. Food Chem 104:163–168CrossRef
36.
Zurück zum Zitat Miguel M, Alvarez Y, López-Fandiño R, Alonso MJ et al (2007) Vasodilator effects of peptides derived from egg white proteins. Regul Peptides 140:131–135CrossRef Miguel M, Alvarez Y, López-Fandiño R, Alonso MJ et al (2007) Vasodilator effects of peptides derived from egg white proteins. Regul Peptides 140:131–135CrossRef
37.
Zurück zum Zitat Manso MA, Miguel M, Even J, Hernández R et al (2008) Effect of the long-term intake of an egg white hydrolysate on the oxidative status and blood lipid profile of spontaneously hypertensive rats. Food Chem 109:361–367CrossRef Manso MA, Miguel M, Even J, Hernández R et al (2008) Effect of the long-term intake of an egg white hydrolysate on the oxidative status and blood lipid profile of spontaneously hypertensive rats. Food Chem 109:361–367CrossRef
38.
Zurück zum Zitat Liu YF, Oey I, Bremer P, Carne A, Silcock P (2017) Bioactive peptides derived from egg proteins: A review. Crit Rev Food Sci 13:1–23 Liu YF, Oey I, Bremer P, Carne A, Silcock P (2017) Bioactive peptides derived from egg proteins: A review. Crit Rev Food Sci 13:1–23
39.
Zurück zum Zitat Sato K, Urado D (2015) in Detection and identification of food-derived peptides in human blood: food-derived short chain peptidomes in human blood, in Genomics, proteomics and metabolomics in nutraceuticals and functional foods: Second Edition, pp 441–452 Sato K, Urado D (2015) in Detection and identification of food-derived peptides in human blood: food-derived short chain peptidomes in human blood, in Genomics, proteomics and metabolomics in nutraceuticals and functional foods: Second Edition, pp 441–452
40.
Zurück zum Zitat Rosendorff C, Black CP, Cannon BJ, Gersh BJ, Gore J, Izzo JL, Kaplan NM, O'Connor CM, O'Gara PT, Oparil S, American Heart Association Council for High Blood Pressure Research, American Heart Association Council on Clinical Cardiology, American Heart Association Council on Epidemiology and Prevention (2007) Treatment of hypertension in the prevention and management of ischemic heart disease: a scientific statement from the American Heart Association council for high blood pressure research and the councils on clinical cardiology and epidemiology and prevention. Circulation 115:2761–2788CrossRef Rosendorff C, Black CP, Cannon BJ, Gersh BJ, Gore J, Izzo JL, Kaplan NM, O'Connor CM, O'Gara PT, Oparil S, American Heart Association Council for High Blood Pressure Research, American Heart Association Council on Clinical Cardiology, American Heart Association Council on Epidemiology and Prevention (2007) Treatment of hypertension in the prevention and management of ischemic heart disease: a scientific statement from the American Heart Association council for high blood pressure research and the councils on clinical cardiology and epidemiology and prevention. Circulation 115:2761–2788CrossRef
Metadaten
Titel
Effect of an egg ovalbumin-derived protein hydrolysate on blood pressure and cardiovascular risk in adults with a mildly elevated blood pressure: a randomized placebo-controlled crossover trial
verfasst von
Alice J. Lucey
Clara Heneghan
Edmund Manning
Paul A. Kroon
Máiréad E. Kiely
Publikationsdatum
03.10.2018
Verlag
Springer Berlin Heidelberg
Erschienen in
European Journal of Nutrition / Ausgabe 7/2019
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-018-1832-9

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