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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Molecular Cancer 1/2012

Effect of cancer-associated mutations in the PlexinB1 gene

Zeitschrift:
Molecular Cancer > Ausgabe 1/2012
Autoren:
Chun Zhou, Oscar Gee-Wan Wong, John R Masters, Magali Williamson
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1476-4598-11-11) contains supplementary material, which is available to authorized users.
Chun Zhou, Oscar Gee-Wan Wong contributed equally to this work.

Competing interests

A patent has been filed by M.W. and J.R.M. for mutations.

Authors' contributions

CZ and OW carried out the molecular genetic studies. JM participated in its design and coordination. MW conceived of the study, coordinated it and drafted the manuscript. All authors read and approved the final manuscript.

Abstract

Background

Semaphorins act as chemotactic cues for cell movement via their transmembrane receptors, plexins. Somatic missense mutations in the plexinB1 gene coupled with overexpression of the protein frequently occur in prostate tumours, indicating a role for plexinB1 in the pathogenesis of prostate cancer.

Results

Two specific mutations found in prostate cancer enhance RhoD binding and one other mutation results in loss of inhibition of Rac-dependent Pak1 phosphorylation and lamellipodia formation and in impairment of trafficking of plexinB1 to the membrane. None of the three characterised mutations affect PDZRhoGEF binding, RhoA activity, the interaction of plexinB1with the oncogenes ErbB2 or c-Met or ErbB2 phosphorylation. The mutations have the net effect of increasing cell motility by blocking plexinB1-mediated inhibition of Rac while enhancing the interaction with RhoD, an anti-migratory factor.

Conclusions

PlexinB1 mutations block plexinB1-mediated signalling pathways that inhibit cell motility.
Zusatzmaterial
Authors’ original file for figure 1
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Authors’ original file for figure 2
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Authors’ original file for figure 3
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Authors’ original file for figure 4
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Literatur
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