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09.01.2020 | Original Article Open Access

Effect of cannabinoid-serotonin interactions in the regulation of neuropeptide Y1 receptors expression in rats: the role of CB1 and 5-HT_2C receptor

Zeitschrift:: Comparative Clinical Pathology

Autoren:: Mohammad Reza Khansari, Negar Panahi, Saeid Hosseinzadeh, Morteza Zendehdel

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Abstract

Neuropeptide Y (NPY) is involved in a diversity of critical functions such as circadian rhythms, energy homeostasis, and appetite regulation in the hypothalamus. It has identified as a crucial participant in adjusting energy intake and energy storage as fat via central neuropeptide Y1 receptor (NPY1R), leading to obesity and metabolic disorders. The present study was expected to investigate the interaction between 2-AG (CB1R agonist), m-CPP (5HT_2CR agonist), SB-242084 (5HT_2CR antagonist), and SR-141716A (CB1R antagonist) by mediating through the NPY1R for treating or preventing obesity, metabolic disorders, and other abnormalities. The expression level of NPY1R mRNA has studied on the rat brain by real-time quantitative PCR assay. Based on our findings, intracerebroventricular (ICV) injection of combined 2-AG (1 μg) + m-CPP (2.5 μg) has antagonistic interaction in the expression of the NPY1R gene (P < 0.001). Moreover, the ICV co-injection of SB-242084 (3 μg) + SR-141716A (1 μg) has antagonistic interaction in the NPY1R gene expression (P < 0.001). Co-administration of 2-AG (1 μg) + SB-242084 (3 μg) amplified NPY1R gene expression (P < 0.001), while the ICV co-injection of m-CPP (2.5 μg) + SR-141716A (1 μg) decreased NPY1R gene expression in the hypothalamus (P < 0.001). These results revealed the interference in cannabinoid and serotonergic systems via CB1 and 5HT_2C receptors in the expression of NPY1R mRNA in the hypothalamic area of rats.

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Titel: Effect of cannabinoid-serotonin interactions in the regulation of neuropeptide Y1 receptors expression in rats: the role of CB1 and 5-HT2C receptor
Autoren:: Mohammad Reza Khansari
Negar Panahi
Saeid Hosseinzadeh
Morteza Zendehdel
Publikationsdatum: 09.01.2020
DOI: https://doi.org/10.1007/s00580-019-03081-z
Verlag: Springer London
Zeitschrift: Comparative Clinical Pathology
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X

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01.02.2020 | Forensische Begutachtung | CME | Ausgabe 1/2020

Effect of cannabinoid-serotonin interactions in the regulation of neuropeptide Y1 receptors expression in rats: the role of CB1 and 5-HT_2C receptor

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