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01.12.2012 | Research | Ausgabe 1/2012 Open Access

World Journal of Surgical Oncology 1/2012

Effect of L-arginine supplement on liver regeneration after partial hepatectomy in rats

Zeitschrift:
World Journal of Surgical Oncology > Ausgabe 1/2012
Autoren:
Tsuyoshi Kurokawa, Jun An, Koji Tsunekawa, Yoshiharu Shimomura, Shunsuke Kazama, Naohisa Ishikawa, Toshiaki Nonami, Satoru Sugiyama
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1477-7819-10-99) contains supplementary material, which is available to authorized users.
Presented by Tsuyoshi Kurokawa

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

TK carried out design of the study and drafted manuscript. JA carried out experimental procedures and data analysis. KT, YS, SK carried out data analysis and reviewed the paper. NI, TN and SS guaranteed the whole study. All authors read and approved the final manuscript.

Abstract

Background

Nitric oxide (NO) has been reported to be a key mediator in hepatocyte proliferation during liver regeneration. NO is the oxidative metabolite of L-arginine, and is produced by a family of enzymes, collective termed nitric oxide synthase (NOS). Thus, administration of L-arginine might enhance liver regeneration after a hepatectomy. Another amino acid, L-glutamine, which plays an important role in catabolic states and is a crucial factor in various cellular and organ functions, is widely known to enhance liver regeneration experimentally. Thus, the present study was undertaken to evaluate the effects of an L-arginine supplement on liver regeneration, and to compared this with supplementation with L-glutamine and L-alanine (the latter as a negative control), using a rat partial hepatectomy model.

Methods

Before and after a 70% hepatectomy, rats received one of three amino acid solutions (L-arginine, L-glutamine, or L-alanine). The effects on liver regeneration of the administered solutions were examined by assessment of restituted liver mass, staining for proliferating cell nuclear antigen (PCNA), and total RNA and DNA content 24 and 72 hours after the operation.

Results

At 72 hours after the hepatectomy, the restituted liver mass, the PCNA labeling index and the DNA quantity were all significantly higher in the L-arginine and L-glutamine groups than in the control. There were no significant differences in those parameters between the L-arginine and L-glutamine groups, nor were any significant differences found between the L-alanine group and the control.

Conclusion

Oral supplements of L-arginine and L-glutamine enhanced liver regeneration after hepatectomy in rats, suggesting that an oral arginine supplement can clinically improve recovery after a major liver resection.
Zusatzmaterial
Authors’ original file for figure 1
12957_2012_1048_MOESM1_ESM.pdf
Authors’ original file for figure 2
12957_2012_1048_MOESM2_ESM.pdf
Authors’ original file for figure 3
12957_2012_1048_MOESM3_ESM.pdf
Literatur
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