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11.10.2018 | Original Article

Effect of NaF, AmF, KF gels and NaF toothpaste combined with a saliva substitute on dentin lesions in vitro

verfasst von: Carolin Walther, Manana Kreibohm, Sebastian Paris, Hendrik Meyer-Lueckel, Peter Tschoppe, Richard Johannes Wierichs

Erschienen in: Clinical Oral Investigations | Ausgabe 5/2019

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Abstract

Objective

The aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany.

Methods

In each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (ΔZ_B). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F⁻ [pH = 6.04] (NaF-gel₁), 12.500 ppm F⁻ [pH = 7.34] (NaF-gel₂), 12.500 ppm F⁻ [pH = 5.82] (AmF-gel), 1450 ppm F⁻ [pH = 7.35] (KF-gel), and 5000 ppm F⁻ [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔZ_E1/ΔΔZ_E2) were calculated between values before and after pH cycling.

Results

Mean (95%CI) ΔZ_B was 3851 (3762;3939) vol% × μm. Except for NaF-gel₂ and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel₂ induced a significant gain in mineral content (p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔZ_E1 and for ΔΔZ_E2 (p < 0.05, Bonferroni post hoc test). However, only NaF-gel₂ and NaF-TP induced remineralization.

Conclusion

Under the in vitro conditions chosen, all fluoride agents could significantly hamper the adverse effects of a demineralizing saliva substitute.

Clinical significance

In combination with a demineralizing saliva substitute, slight mineral gain was only observed for neutral NaF-gel₂ and 5000 ppm F⁻ toothpaste.

Ahadian H, Yassaei S, Bouzarjomehri F, Ghaffari Targhi M, Kheirollahi K (2017) Oral complications of the Oromaxillofacial area radiotherapy. Asian Pac J Cancer Prev 18:721–725. https://doi.org/10.22034/APJCP.2017.18.3.721 CrossRefPubMedPubMedCentral

Atkinson JC, Grisius M, Massey W (2005) Salivary hypofunction and xerostomia: diagnosis and treatment. Dent Clin N Am 49:309–326. https://doi.org/10.1016/j.cden.2004.10.002 CrossRefPubMed

Almstahl A, Wikstrom M (2003) Electrolytes in stimulated whole saliva in individuals with hyposalivation of different origins. Arch Oral Biol 48:337–344CrossRefPubMed

Tschoppe P, Wolgin M, Pischon N, Kielbassa AM (2010) Etiologic factors of hyposalivation and consequences for oral health. Quintessence Int 41:321–333PubMed

Vissink A, Jansma J, Spijkervet FK, Burlage FR, Coppes RP (2003) Oral sequelae of head and neck radiotherapy. Crit Rev Oral Biol Med 14:199–212CrossRefPubMed

Kielbassa AM, Hinkelbein W, Hellwig E, Meyer-Luckel H (2006) Radiation-related damage to dentition. Lancet Oncol 7:326–335. https://doi.org/10.1016/S1470-2045(06)70658-1 CrossRefPubMed

Vissink A, Burlage FR, Spijkervet FK, Jansma J, Coppes RP (2003) Prevention and treatment of the consequences of head and neck radiotherapy. Crit Rev Oral Biol Med 14:213–225CrossRefPubMed

Meyer-Lueckel H, Colfen H, Verch A, Tschoppe P (2010) Effects of carboxymethyl cellulose-based saliva substitutes with varying degrees of saturation with respect to calcium phosphates on artificial enamel lesions. Caries Res 44:127–134. https://doi.org/10.1159/000302901 CrossRefPubMed

Zimmermann JS, Niehoff P, Wilhelm R, Schneider R, Kovacs G, Kimmig B (1998) Prevention and therapy of acute radiation-related morbidity of the skin and mucosa. II, recommendations of the literature. Strahlenther Onkol 174:193–199CrossRefPubMed

10.

Momm F, Volegova-Neher NJ, Schulte-Monting J, Guttenberger R (2005) Different saliva substitutes for treatment of xerostomia following radiotherapy. A prospective crossover study. Strahlenther Onkol 181:231–236. https://doi.org/10.1007/s00066-005-1333-7 CrossRefPubMed

11.

Zandim-Barcelos DL, Kielbassa AM, Sampaio JE, Tschoppe P (2015) Saliva substitutes in combination with high-fluoride gel on dentin remineralization. Clin Oral Investig 19:289–297. https://doi.org/10.1007/s00784-014-1264-8 CrossRefPubMed

12.

Tschoppe P, Meyer-Lueckel H (2012) Effects of regular and highly fluoridated toothpastes in combination with saliva substitutes on artificial enamel caries lesions differing in mineral content. Arch Oral Biol 57:931–939. https://doi.org/10.1016/j.archoralbio.2012.02.010 CrossRefPubMed

13.

Marinho VC, Worthington HV, Walsh T and Chong LY (2015) Fluoride gels for preventing dental caries in children and adolescents. Cochrane Database Syst Rev:CD002280. doi: https://doi.org/10.1002/14651858.CD002280.pub2

14.

Nieuw Amerongen AV, Veerman EC (2003) Current therapies for xerostomia and salivary gland hypofunction associated with cancer therapies. Support Care Cancer 11:226–231. https://doi.org/10.1007/s00520-002-0409-5 CrossRefPubMed

15.

Tschoppe P, Meyer-Lueckel H (2011) Mineral distribution of artificial dentinal caries lesions after treatment with fluoride agents in combination with saliva substitutes. Arch Oral Biol 56:775–784. https://doi.org/10.1016/j.archoralbio.2011.01.002 CrossRefPubMed

16.

Tschoppe P, Siegel A, Meyer-Lueckel H (2010) Saliva substitutes in combination with highly concentrated fluorides and brushing: in vitro effects on enamel subsurface lesions. Caries Res 44:571–578. https://doi.org/10.1159/000321656 CrossRefPubMed

17.

Zandim DL, Tschoppe P, Sampaio JE, Kielbassa AM (2011) Effect of saliva substitutes in combination with fluorides on remineralization of subsurface dentin lesions. Support Care Cancer 19:1143–1149. https://doi.org/10.1007/s00520-010-0924-8 CrossRefPubMed

18.

Wierichs RJ, Zelck H, Doerfer CE, Appel P, Paris S, Esteves-Oliveira M, Meyer-Lueckel H (2017) Effects of dentifrices differing in fluoride compounds on artificial enamel caries lesions in vitro. Odontology 105:36–45. https://doi.org/10.1007/s10266-016-0233-x CrossRefPubMed

19.

Arends J and ten Bosch JJ (1992) Demineralization and remineralization evaluation techniques. J Dent Res 71 Spec No:924–8

20.

Wierichs RJ, Lausch J, Meyer-Lueckel H, Esteves-Oliveira M (2016) Re- and demineralization characteristics of enamel depending on baseline mineral loss and lesion depth in situ. Caries Res 50:141–150. https://doi.org/10.1159/000444537 CrossRefPubMed

21.

Wierichs RJ, Stausberg S, Lausch J, Meyer-Lueckel H, Esteves-Oliveira M (2018) Caries-preventive effect of NaF, NaF plus TCP, NaF plus CPP-ACP, and SDF varnishes on sound dentin and artificial dentin caries in vitro. Caries Res 52:199–211. https://doi.org/10.1159/000484483 CrossRefPubMed

22.

Meyer-Lueckel H, Wierichs RJ, Gninka B, Heldmann P, Dorfer CE, Paris S (2015) The effect of various model parameters on enamel caries lesions in a dose-response model in situ. J Dent 43:1261–1267. https://doi.org/10.1016/j.jdent.2015.08.003 CrossRefPubMed

23.

Esteves-Oliveira M, Santos NM, Meyer-Lueckel H, Wierichs RJ, Rodrigues JA (2017) Caries-preventive effect of anti-erosive and nano-hydroxyapatite-containing toothpastes in vitro. Clin Oral Investig 21:291–300. https://doi.org/10.1007/s00784-016-1789-0 CrossRefPubMed

24.

Wierichs RJ, Westphal S, Lausch J, Meyer-Lueckel H, Esteves-Oliveira M (2018) Influence of highly concentrated fluoride dentifrices on remineralization characteristics of enamel in vitro. Clin Oral Investig 22:2325–2334. https://doi.org/10.1007/s00784-018-2333-1 CrossRefPubMed

25.

Wierichs RJ, Kogel J, Lausch J, Esteves-Oliveira M, Meyer-Lueckel H (2017) Effects of self-assembling peptide P11-4, fluorides, and caries infiltration on artificial enamel caries lesions in vitro. Caries Res 51:451–459. https://doi.org/10.1159/000477215 CrossRefPubMed

26.

ten Cate JM, Exterkate RA, Buijs MJ (2006) The relative efficacy of fluoride toothpastes assessed with pH cycling. Caries Res 40:136–141. https://doi.org/10.1159/000091060 CrossRefPubMed

27.

ten Cate JM, Buijs MJ, Miller CC, Exterkate RA (2008) Elevated fluoride products enhance remineralization of advanced enamel lesions. J Dent Res 87:943–947. https://doi.org/10.1177/154405910808701019 CrossRefPubMed

28.

Duckworth RM, Gao XJ (2006) Plaque as a reservoir for active ingredients. Monogr Oral Sci 19:132–149. https://doi.org/10.1159/000090589 CrossRefPubMed

29.

Wegehaupt FJ, Attin T (2010) The role of fluoride and casein phosphopeptide/amorphous calcium phosphate in the prevention of erosive/abrasive wear in an in vitro model using hydrochloric acid. Caries Res 44:358–363. https://doi.org/10.1159/000316542 CrossRefPubMed

30.

Yamazaki H, Margolis HC (2008) Enhanced enamel remineralization under acidic conditions in vitro. J Dent Res 87:569–574. https://doi.org/10.1177/154405910808700612 CrossRefPubMed

31.

Brighenti FL, Delbem AC, Buzalaf MA, Oliveira FA, Ribeiro DB, Sassaki KT (2006) In vitro evaluation of acidified toothpastes with low fluoride content. Caries Res 40:239–244. https://doi.org/10.1159/000092232 CrossRefPubMed

32.

Alves KM, Pessan JP, Brighenti FL, Franco KS, Oliveira FA, Buzalaf MA, Sassaki KT, Delbem AC (2007) In vitro evaluation of the effectiveness of acidic fluoride dentifrices. Caries Res 41:263–267. https://doi.org/10.1159/000101915 CrossRefPubMed

33.

Backfolk K, Lagerge S, Rosenholm JB, Eklund D (2002) Aspects on the interaction between sodium carboxymethylcellulose and calcium carbonate and the relationship to specific site adsorption. J Colloid Interface Sci 248:5–12. https://doi.org/10.1006/jcis.2001.8195 CrossRefPubMed

34.

Altenburger MJ, Schirrmeister JF, Wrbas KT, Klasser M, Hellwig E (2008) Fluoride uptake and remineralisation of enamel lesions after weekly application of differently concentrated fluoride gels. Caries Res 42:312–318. https://doi.org/10.1159/000148164 CrossRefPubMed

35.

Lennon AM, Pfeffer M, Buchalla W, Becker K, Lennon S, Attin T (2006) Effect of a casein/calcium phosphate-containing tooth cream and fluoride on enamel erosion in vitro. Caries Res 40:154–157. https://doi.org/10.1159/000091063 CrossRefPubMed

36.

Lagerweij MD, ten Cate JM (2006) Acid susceptibility at various depths of pH-cycled enamel and dentine specimens. Caries Res 40:33–37. https://doi.org/10.1159/000088903 CrossRefPubMed

37.

Brown WE, Gregory TM, Chow LC (1977) Effects of fluoride on enamel solubility and cariostasis. Caries Res 11(Suppl 1):118–141CrossRefPubMed

38.

Lippert F, Juthani K (2015) Fluoride dose-response of human and bovine enamel artificial caries lesions under pH-cycling conditions. Clin Oral Investig 19:1947–1954. https://doi.org/10.1007/s00784-015-1436-1 CrossRefPubMed

Titel: Effect of NaF, AmF, KF gels and NaF toothpaste combined with a saliva substitute on dentin lesions in vitro
verfasst von: Carolin Walther
Manana Kreibohm
Sebastian Paris
Hendrik Meyer-Lueckel
Peter Tschoppe
Richard Johannes Wierichs
Publikationsdatum: 11.10.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 5/2019
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2687-4

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Effect of NaF, AmF, KF gels and NaF toothpaste combined with a saliva substitute on dentin lesions in vitro

Abstract

Objective

Methods

Results

Conclusion

Clinical significance

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Abstract

Objective

Methods

Results

Conclusion

Clinical significance

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