Effect of storage time on transforming growth factor beta 1 and vascular endothelial growth factor subunit A concentration in canine platelet-rich fibrin

Platelet-rich fibrin (PRF) has gained more interests as an alternative modality for periodontal therapy due to its anti-inflammatory effect from platelet cytokines embedded in fibrin network. One of the factors that affect efficacy of PRF is the concentration of the incorporated cytokines. However, there is inadequate information regarding PRF preservation. Thus, this study was conducted to build on the knowledge in canine study regarding cytokine concentration and also the effect of storage condition. Transforming growth factor beta 1 (TGF-β1) and vascular endothelial growth factor subunit A (VEGF-A) in canine’s blood (n = 10) using advanced PRF plus technique were determined by means of enzyme-linked immunosorbent assay (ELISA) at 3 different time points; immediately (baseline), 7 days after storage at − 20 °C, and 14 days after storage at − 20 °C. Data on concentration at each time points were compared. The result revealed a pattern of time-dependent decay; TGF-β1 decreased from 171.50 ng/ml (at baseline) to 125.8 ng/ml (day 7) and 88.9 ng/ml (day 14), VEGF-A decreased from 88.21 pg/ml (at baseline) to 70.82 pg/ml (day 7) and 63.44 pg/ml (day 14). These findings indicated that PRF provided the highest cytokine concentration while used promptly after preparation.