Background
Inflammation of the central nervous system (CNS) during multiple sclerosis (MS) or the animal model experimental autoimmune encephalomyelitis (EAE) is believed to be mediated by autoreactive T cells that are primed in the periphery and infiltrate into the CNS through the blood-brain barrier (BBB) [
1,
2]. Among T cells, predominantly, IFN-γ-producing Th1 cells and IL-17-producing Th17 cells are key players in the pathogenesis of MS and EAE [
3,
4]. Much of our understanding has been obtained from adoptive transfer experiments in rodent EAE models, which suggest that both Th1 and Th17 cells can mediate the disease in the CNS albeit with a varying degree of severity [
5‐
8]. Due to the complexity of the neuroinflammatory process, there is only little knowledge available on the individual contribution of pathogenic Th1 and Th17 cells in regulating glial cell function in the CNS.
Of the CNS-resident cells, microglia and astrocytes are known to play a central role in regulating the neuroinflammatory process [
9‐
12]. Infiltrating effector T cells are in constant crosstalk with resident glial cells, and recently, we have demonstrated that effector molecules secreted by Th1 cells but not Th17 cells influence the phenotype and function of microglia [
13]. This was rather puzzling, considering the fact that Th17 cells are highly pathogenic and microglia are highly immunoreactive cell types in the CNS.
Only little is known about the crosstalk between T cells and astrocytes, the major glial cell type of the brain. Previously, astrocytes were considered to be involved in the structural framework of neural tissue while their immunoreactive properties have been underestimated. Furthermore, their intimate association with the BBB makes them one of the first glial types to encounter cells infiltrating into the CNS [
14]. Subsequently, astrocyte activation regulates microglial recruitment and leukocyte trafficking in the CNS during neuroinflammatory conditions [
15,
16]. In the past two decades, the contribution of astrocytes to pro- and anti-inflammatory processes in the CNS has gained prominence [
17‐
20]. The importance of astrocytes in regulating CNS inflammation has been demonstrated by several studies which employed strategies such as ablation of reactive astrocytosis or blocking of selected receptor signaling specifically on astrocytes [
10,
12,
21‐
23]. Upon activation, astrocytes upregulate major histocompatibility class (MHC)-I, MHC-II and co-stimulatory molecules on their surface, which highlights their ability to interact and present antigens to T cells [
24‐
26]. Nevertheless, there is only limited knowledge about how the effector molecules released by inflammatory Th1 and Th17 cells regulate the function of astrocytes. In this study, we identified astrocytes as targets of Th17 cells in the CNS and studied the ability of effector molecules released by Th1 and Th17 cells to influence their phenotype and function.
Methods
Mice
C57BL/6 mice were obtained from Charles River (Sulzfeld, Germany) and housed under specific pathogen-free conditions in the central animal facility of Hannover Medical School (MHH), Germany. α4
flox/flox
has been described previously [
27].
CD4 Cre mice were obtained from The Jackson Laboratory (Bar Harbor, ME, USA). CD4
+ T cell-conditional α4 integrin-deficient (α4
−/−) mice were generated by crossing α4
flox/flox
mice with
CD4 Cre mice [
28]. For EAE experiments, we obtained glial fibrillary acidic protein (GFAP) HSV-thymidine kinase (TK) mice on C57BL/6 background from The Jackson Laboratory (Bar Harbor, ME, USA) and the control wild-type C57BL/6 mice in this case were from Taconic (Taconic Europe, Ejby, Denmark). Animal experiments were performed according to international guidelines on the use of laboratory animals [
29].
Experimental autoimmune encephalomyelitis
EAE was induced in GFAP HSV-TK mice, α4
−/− mice, and control B6 mice by immunization with MOG
35–55 peptide in complete Freund’s adjuvant, as described previously [
12,
28]. Mice were immunized at two subcutaneous sites and received a total of 100 μg peptide and 200 or 250 μg adjuvant. Additionally, 15 ng/g or 200 ng pertussis toxin was administered i.p. on days 0 and 2. GFAP HSV-TK and respective controls mice were scored on a 6-point scale as follows: 0, no symptoms; 0.5, partial loss of tail tonus; 1, complete loss of tail tonus; 2, difficulty to right, 3, paresis in one or both hind legs; 4, paralysis in one or both hind legs; 5, front limb paresis; and 6, moribund. All mice used in the experiments were sacrificed 7 days after the onset of clinical symptoms. Ataxic EAE in α4
−/− mice was scored, by four clinical subtests and categories of ledge walking, hindlimb clasp, gait ataxia, and kyphosis with a maximum of 3 points in each category, resulting in a potential maximum score of 12 points [
30]. Clinical signs of classical EAE in respective wild-type controls mice were assessed as reported [
31].
Antibodies and reagents
Antibodies specific for mouse, anti-CD4 PerCP Cy5.5 (clone: RM 4.5), anti-CD8 APC (clone 53.6.7), anti-CD11c APC (clone: N418), anti-IFN-γ APC (clone: XMG1.2), anti-CD62L APC eFluor 780 (clone: MEL-14), anti-F4/80 APC (clone: BM8), anti-CD3 (unconjugated, clone: 145-2C11), anti-CD28 (unconjugated, clone: 37.51), and fixable viability dye eFluor 506 were purchased from eBioscience (Frankfurt, Germany). Antibodies to anti-CD25 APC (clone: PC61), anti-IL-17A Pacific Blue (clone: TC11-18H10.1), anti-CD11b PE (clone: M1/70), anti-B220 APC (clone RA3-6B2), and anti-IL-10 FITC (JES5-16E3) were purchased from BioLegend (San Diego, CA, USA). Unconjugated, anti-IFN-γ (clone: XMG1.2), and anti-IL-4 (clone: 11B11) and anti-IL-2 (clone JES6-1A12) were obtained from Bio X Cell (NH, USA). Recombinant murine IL-6, IL-1β, granulocyte macrophage colony-stimulating factor (GM-CSF), and porcine TGF-β1 were purchased from R&D Systems (Wiesbaden-Nordenstadt, Germany) whereas recombinant murine IFN-γ, TNF-α, and IL-12p70 were from PeproTech (Hamburg, Germany). Recombinant murine IL-17A was obtained from BioLegend (San Diego, CA, USA).
In vitro differentiation of Th1 and Th17 cells
Naïve CD4
+CD25
− cells were differentiated in vitro into Th1 and Th17 cells as previously described [
13] with slight modifications. Briefly, after enrichment of CD4
+ T cells from the spleen and lymph nodes of C57BL/6 mice using CD4
+ T cell enrichment kit (BD Biosciences), naïve CD4
+CD62L
hiCD25
− cells were sort purified using MoFlo (Beckman Coulter) or FACSAria (BD Biosciences). Cells (5.0 × 10
5/ml) were stimulated with plate-bound anti-CD3 (2 μg/ml) and anti-CD28 (2 μg/ml) in 12-well plates (Corning Life Science, Acton, MA, USA) in complete Iscove’s Modified Dulbecco’s Medium (IMDM, 10% FCS, 1 mM sodium pyruvate, 50 μM β-mercaptoethanol, 25 mM HEPES, and non-essential amino acids) supplemented with either Th1-polarizing factors IL-12 (20 ng/ml) and anti-IL-4 (10 μg/ml) or with Th17-polarizing factors TGF-β1 (2 ng/ml), IL-6 (30 ng/ml), TNF-α (20 ng/ml), IL-1β (10 ng/ml), anti-IL-2 (10 μg/ml), and anti-IFN-γ (10 μg/ml). After 6 days of culture, Th1 and Th17 cells were harvested and restimulated in 12-well plates coated with anti-CD3 and anti-CD28 antibodies for 6 h. Supernatants devoid of cells were collected and stored at − 80 °C until further use.
Primary mouse mixed glial cultures
Primary cultures of mixed glial cells were prepared from brains of postnatal 1–3-day-old C57BL/6 mice as described [
13]. Briefly, the brains were freed from meninges and digested enzymatically with 0.1% trypsin (Sigma-Aldrich) and 0.25% DNase (Roche, Mannheim, Germany). Single cell suspensions obtained from the digested brains were seeded into poly-
l-lysine-coated T-75-mm
2 culture flasks in complete DMEM (DMEM +
l-glutamine + 4.5 g/L
d-glucose; Gibco
®, Darmstadt, Germany) supplemented with 10% fetal calf serum (FCS), 50 U/ml penicillin, and 50 μg/ml streptomycin (all from Biochrom AG, Berlin, Germany). Microglia were harvested from the confluent cultures by shaking the culture flasks at 37 °C for 40 min at 180 rpm in an orbital shaker. Remaining microglia and proliferating oligodendrocyte precursor cells were eliminated by overnight shaking at 37 °C and 170 rpm in an orbital shaker, followed by cytosine arabinoside (AraC; 100 μM; Sigma-Aldrich) treatment for 3 days. Astrocytes were harvested from the culture flasks by mild trypsinization and were replated into six-well plates at a density of 3 × 10
5 cells per well. Confluent astrocyte cultures were shaken at 37 °C and 180 rpm in an orbital shaker for 4 h to eliminate any remaining contaminating microglia. Astrocytes obtained in this way were referred to as highly enriched as they only had < 3% of microglial contamination (CD11b
+ cells).
Stimulation of astrocytes with Th1- and Th17-derived supernatants and recombinant cytokines
Confluent astrocyte cultures as obtained in the above procedure were treated for 16 h with Th1- or Th17-derived culture supernatants diluted with an equal volume of complete DMEM. In all experiments, medium controls refer to T cell culture medium (complete IMDM) collected, frozen, and diluted with an equal volume of complete DMEM just before the treatment of astrocytes. In some experiments, astrocytes were treated for 16 h with recombinant murine IFN-γ (50 ng/ml), TNF-α (10 ng/ml), GM-CSF (5 ng/ml), and IL-17A (50 ng/ml) either individually or in combination with others.
Reverse transcription polymerase chain reaction
RNA was isolated from the cell pellet using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. Equal amounts of RNA (750–1000 ng) were subsequently transcribed into complementary DNA (cDNA) with the High-Capacity cDNA Reverse Transcription Kit (No. 4368814; Applied Biosystems
®; Life Technologies GmbH, Darmstadt, Germany). For gene expression analysis, quantitative real-time PCR was performed using the StepOne™ Real-Time PCR System and appropriate TaqMan probes (Applied Biosystems, see Additional file
1). The ΔΔCt method was applied to determine differences in the expression between astrocytes treated with medium and Th1 and Th17 supernatants. For determination of expression of retinoic acid orphan receptor c (
Rorc) messenger RNA (mRNA) from the spinal cord, 1 μg of RNA was used for cDNA synthesis. Changes in mRNA expression levels were calculated after normalization to hypoxanthine phosphoribosyltransferase (
Hprt1) and glyceraldehyde 3-phosphate dehydrogenase (
Gapdh).
ELISA
Supernatants of astrocytes cultured in the medium and Th1 and Th17 supernatants, respectively, were collected and stored at − 80 °C until further use. MCP-1/C-C chemokine ligand 2 (CCL2) (R&D Systems), CCL20 (R&D Systems), and IL-6 (Thermo Fisher Scientific) were measured in the culture supernatants using enzyme-linked immunosorbent assay (ELISA) kits for mouse and carried out according to the manufacturer’s instructions. A standard curve was generated as instructed using the standards provided in the kit. The standard curve was calculated by a computer-generated four-parameter log (4-PL) fit curve.
Histology
Animals were sacrificed at the peak of the disease and perfused with cold PBS followed by 4% paraformaldehyde fixation (pH 7.4). The brain and spinal cord were prepared separately, embedded in Tissue-Tek (Sakura), and cryopreserved in liquid nitrogen. Twelve-micrometer-thick coronal cortical brain, sagittal brainstem, and transverse lumbar spinal cord sections were prepared using a cryotome. For immunofluorescence, sections were thawed and air-dried and, following 5-min rehydration with PBS, were stained with primary rabbit polyclonal anti-GFAP (Dako) or polyclonal rabbit Iba1 (Wako) antibodies in 0.1% Triton PBS for 2 h at room temperature (RT). After thorough washing, sections were incubated with Alexa Fluor 488- and Alexa Fluor 555-conjugated goat-anti-rabbit secondary antibodies, respectively, in 0.1% Triton PBS for 1 h at RT. After washing, slides were mounted with DAPI in Mowiol. Images were taken using a microscope (Olympus BX41) with camera.
Transmigration of microglia
Astrocytes at 5 × 104 cells per well were plated into the lower chamber of 24-well Transwell plates and cultured until they reached confluence. Confluent astrocyte cultures were treated with medium and Th1 and Th17 supernatants (diluted with an equal volume of DMEM 10% FCS) for 12 h. Following this, the cultures were washed to remove the stimuli; fresh complete DMEM was added and incubated for additional 4 h. Microglia harvested after shaking the mixed glial cultures were added to the Transwell inserts (8.0 μm pore, 24-well format; Costar®, Corning, NY, USA) at 7 × 104 cells per insert, and the inserts were placed into the chambers containing astrocytes. After 2 h of incubation at 37 °C, microglia on the upper side of the insert were removed by using a cotton swab. The filters were then fixed with 4% PFA, stained with DAPI (1:2000), and mounted onto a glass slide with Mowiol. The number of cells that had transmigrated to the lower side of the membrane was counted (ten random fields/filter) at × 20 magnification using an Olympus BX41 fluorescence microscope.
Transendothelial migration of T cells
Primary C57BL/6 brain microvascular endothelial cells (BMECs) and reagents needed for culturing them were purchased from Cell Biologics (Chicago, USA). Cells used for these experiments were from passages P5–P9. Astrocytes at 3 × 105 cells per well were cultured in six-well plates in complete DMEM until they reached confluence. In parallel, BMECs (2.5 × 105 cells per insert) were cultured on Transwell inserts (3.0 μm pore, polycarbonate membrane, six-well format; Costar®, Corning, NY, USA) coated with gelatin-based coating solution until they formed a confluent monolayer. Astrocytes were treated with medium and Th1- and Th17-derived supernatants (diluted in equal volumes of complete DMEM) for 12 h and then washed thoroughly to remove the T cell supernatants. Inserts with BMEC monolayers were transferred to the chamber containing astrocytes and co-cultured for an additional period of 4 h. To study T cell migration, a total of 2 × 105 Th1 and Th17 cells that were restimulated on anti-CD3/anti-CD28-coated plates for 6 h were added on top of the BMEC monolayers. After 12 h, inserts were removed; culture medium in the lower chamber containing transmigrated T cell was collected, stained with anti-mouse CD4 PerCP Cy5.5, and analyzed on FACSCalibur (BD Biosciences). Each sample was acquired completely, and the cell counts in the CD4+ gate were used to assess the transendothelial migration of T cells.
Phagocytosis assay
Uptake of latex beads by microglia was measured as described [
32] with slight modifications. Briefly, 7.5 × 10
4 microglia were seeded on to astrocytes that were previously treated with medium and Th1- and Th17-derived supernatants. Th1- and Th17-derived supernatants were washed off before adding microglia. After 12 h of co-culture with astrocytes, 10
7 Fluoresbrite™ YG carboxylate microspheres (1 μm; Polysciences, Warrington, USA) were added to the cells and incubated at 37 °C for 1 h. In parallel, cells were also incubated with beads on ice and this served as negative (4 °C) control. After thoroughly washing away non-phagocytosed beads with ice-cold PBS, cells were harvested and stained with anti-mouse CD11b APC (M1-70), and phagocytosis was measured on a flow cytometer (FACSCalibur; BD Biosciences). Shift in mean fluorescence intensity (MFI) resulting from uptake of fluorescent beads was used as a measure to assess phagocytosis. Active phagocytosis was calculated by subtracting the MFI measured in 4 °C controls from the MFI measured in samples incubated at 37 °C.
Statistical analysis
All statistical analyses were conducted using GraphPad Prism 5.0 (GraphPad Software). All data are expressed as group mean ± SEM. All experiments were performed multiple times (n ≥ 4), and the data obtained was analyzed using the Kruskal-Wallis test unless otherwise stated. For analyzing EAE data, we used the Mann-Whitney test. Results were considered statistically significant at p < 0.05.
Discussion
Previously, we have demonstrated that only effector molecules released by Th1 cells had direct influence on microglia, whereas effector molecules of Th17 cells show no direct effects on microglia [
13]. In this study, we identified astrocytes as one of the targets of Th17 effectors. We observed that during EAE, infiltration of Th17 cells alone was sufficient to induce astrogliosis in the brain. Furthermore, we could demonstrate that factors derived from Th1 and Th17 cells acted on astrocytes and triggered a pro-inflammatory cytokine and chemokine response that assisted the recruitment of microglia and transendothelial migration of Th17 cells.
The current knowledge on Th1 and Th17 cells in MS pathogenesis has come mainly from EAE models where individual antigen-specific Th1 and Th17 cells were adoptively transferred into the mice. However, plasticity associated with adoptively transferred T cells is a major limitation in understanding the contributions of specific effectors in driving the neuroinflammation [
34‐
36]. Migration of effector Th1 and Th17 cells into the CNS is assisted by a distinct set of chemokine receptors and integrins. While the integrin VLA4 (α4β1) is indispensable for Th1 migration, Th17 cells most likely depend on C-C chemokine receptor 6 (CCR6) and LFA-1 [
28,
37]. Earlier work has shown that interfering with specific integrins on CD4
+ T lymphocytes can modulate CNS infiltration of Th1 and Th17 cells [
28]. Conditional knockout of α4 integrin in CD4 T lymphocytes (α4
−/−) causes an atypical EAE in mice with predominant infiltration of Th17 cells and not Th1 cells into the brainstem, cerebellum, and forebrain [
28]. Assessment of glial reactions in wild-type and α4
−/− mice that were subjected to EAE provided us the information of potential targets of Th17 cells in the CNS. Here, astrogliosis and microgliosis were more pronounced in the lumbar spinal cord sections of wild-type mice and less prominent in α4
−/− mice. This could be explained by the fact that infiltration of both Th1 and Th17 cells into the spinal cord is drastically impaired in α4
−/− mice [
28]. In contrast, marked infiltration of Th17 cells but not Th1 cells was detected in the cerebellum and brainstem of α4
−/− mice [
28]. Analyzing the glial reaction of the cerebellum, we detected comparable astrogliosis, whereas the density of microglia (Iba1
+) was reduced and its phenotype was strikingly different in α4
−/− mice despite pronounced infiltrates of Th17 cells. These findings strongly suggest that Th17 cells and their effector molecules are capable of activating astrocytes whereas microglia are less responsive to these cells.
We further studied if effectors of Th1 and Th17 cells had any direct influence on astrocyte activation. Astrocytes are an important source of neurotrophic factors, and downregulating their expression can trigger neurodegeneration [
38]. Here, we observed that Th1-derived factors significantly downregulated the expression of key neurotrophic factors like NGF, BDNF, and CNTF in astrocytes. Similarly, expression of IGF-1, a growth factor involved in the protection of neurons against oxidative stress, is also downregulated, suggesting that effectors of Th1 cells trigger neurodegeneration by suppressing the production of neurotrophic factor by astrocytes. In contrast, Th17-derived factors had no influence on the expression of neurotrophic factors in astrocytes.
Existing evidence suggests that IFN-γ and IL-17, the key cytokines secreted by Th1 and Th17 cells, respectively, are capable of regulating astrocyte function [
25,
39‐
41]. Both Th1- and Th17-derived factors acted on astrocytes and induced a strong pro-inflammatory response where expression of IL-1β, IL-6, and NOS2 mRNA was upregulated by several folds. In addition, we observed a nearly twofold reduction in the expression of the anti-inflammatory factor IL-10 in astrocytes treated with Th1 and Th17 supernatants. Therefore, we believe that effector molecules secreted by Th1 and Th17 cells suppress an anti-inflammatory response and trigger a potent pro-inflammatory response in astrocytes. Although we have previously characterized Th1 and Th17 supernatants in terms of their cytokine profile [
13], it is not known which effector molecules were responsible for driving astrocyte activation. It is noteworthy that while IFN-γ remains the major effector of Th1 cells, astrocyte activation is increased several folds when it is combined with other factors such as TNF and GM-CSF (see Additional file
3). IL-17 is the only major effector detected in our Th17 supernatants along with little amounts of TNF-α. Nevertheless, IL-17 alone had no impact on astrocytes. Interestingly, IL-17 appears to synergize with TNF-α, since we observed increased expression of IL-6 and CCL20 mRNA only when astrocytes were treated with a combination of these cytokines (see Additional file
3). Few studies in the past have reported such synergy between IL-17 and TNF-α on other cell types [
42,
43].
We also observed that Th1-derived supernatants largely enhanced the mRNA expression of CCL2, CXCL10, and CXCL12, whereas CCL20 expression was highly upregulated in astrocytes treated with Th17-derived supernatants. This is an indication that effector molecules of Th1 and Th17 cells induce a selective chemokine response by astrocytes. Chemokines and their receptors act as amplifiers of neuroinflammation by assisting recruitment of immune cells from the periphery and microglia to the inflammatory foci [
44]. We have previously shown that astrocytes are essential for recruitment of microglia for myelin clearance during cuprizone-induced demyelination and remyelination [
21]. Similarly, we observed that astrocytes treated with Th1- or Th17-derived supernatants enhanced microglial migration towards astrocytes. Interestingly, only microglia that migrated towards Th1-treated astrocytes show enhanced phagocytosis. Microglial phagocytosis can have beneficial and detrimental effects in the CNS and can be differentially regulated by several factors [
45]. One such factor, TNF-α is known to enhance the phagocytic activity of microglia [
46] and we have observed that only astrocytes treated with Th1-derived supernatants show enhanced expression of TNF-α.
CCL20 is constitutively expressed by the cells in the choroid plexus and is considered to be the gateway for T cells into the CNS [
37,
47]. A few studies suggest that CCR6, a receptor for CCL20, is expressed specifically on Th17 and regulatory T cells and not on Th1 cells [
37,
48]. Our own experience suggests that CCR6 is also expressed on Th1 cells [
49]. Nonetheless, we hypothesized that Th1 and Th17 cells might activate astrocytes and play a role in the recruitment of a second wave of Th1 and Th17 cells. We first corroborated this hypothesis using an in vitro model where we tested transendothelial migration of activated Th1 and Th17 cells towards astrocytes treated with Th1 and Th17 supernatants. Th1 cells crossed the endothelial barrier and were not dependent on the activation of the astrocytes. However, increased transendothelial migration of Th17 cells was observed only in response to astrocytes treated with either Th1- or Th17-derived supernatants.
Previously, we and others have shown that ablation of reactive astrocytes exacerbated clinical signs of EAE [
12,
23]. In this model, we detected relatively lower expression of
Rorc mRNA in the spinal cord of mice where astrocytes were depleted at the onset EAE, thus supporting our above findings that astrocytes are crucial for recruitment of Th17 cells into the CNS. Although we observe reduced Th17 signal and more severe EAE in the absence of reactive astrocytes, it must be remembered that astrocytes are active components of the BBB where they form the
glia limitans and control the trafficking of all cells through the BBB. Compromising the BBB by depleting astrocytes leads to excess of myeloid infiltrates into the CNS, thus triggering severe neuroinflammation, and this would override the effects mediated by Th1 or Th17 cells in this model.