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01.12.2020 | Original Article

Effects of age and sex on osteocalcin and bone-specific alkaline phosphatase—reference intervals and confounders for two bone formation markers

verfasst von: Sarah Seberg Diemar, Line Tang Møllehave, Nadia Quardon, Louise Lylloff, Betina Heinsbæk Thuesen, Allan Linneberg, Niklas Rye Jørgensen

Erschienen in: Archives of Osteoporosis | Ausgabe 1/2020

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Abstract

Summary

Bone formation markers bone-specific alkaline phosphatase and osteocalcin are used in many clinical situations. Therefore, we calculated reference intervals for the two markers and investigated how they are influenced by several factors including sex and age. Furthermore, we established clinically relevant reference intervals for the two markers.

Objective

The bone turnover markers (BTMs), bone-specific alkaline phosphatase (bone ALP), and osteocalcin (OC), are frequently measured formation markers. The purpose of this study was to establish reference intervals (RIs) for the two BTMs in a general adult Danish population.

Methods

Bone ALP and OC were measured on the iSYS (IDS Plc) automatic analyzer in samples from the Danish Health2006 5-year follow-up study on serum from 2308 participants (54% women, age range 24–76). Participants with self-reported diagnosis of osteoporosis or receiving hormonal replacement were excluded from analyses while participants on hormonal contraceptives were included.

Results

The geometric mean and 95%RI for bone ALP were 13.9 μg/L (7.6–25.6) for men and 13.8 μg/L (7.0–27.4) for women, while for OC 16.0 μg/L (7.5–34.4) for men and 18.6 μg/L (8.1–42.9) for women. Levels of bone ALP increased with increasing age (β 1.004, p < 0.001), while female sex had no effect. OC levels decreased with increasing age (β 0.998, p = 0.009) and increased with female sex (β 1.104, p < 0.001). Based on our findings, we propose for bone ALP and OC three clinical RIs for men based on age and three clinical RI for women based on age and menopausal status.

Conclusion

The RI for bone ALP and OC varies with age and sex and the BTMs are influenced differently by the two factors. Consequently, the need for establishing valid RIs is of great importance before the full potential of BTM can be used in clinical practice.

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Titel: Effects of age and sex on osteocalcin and bone-specific alkaline phosphatase—reference intervals and confounders for two bone formation markers
verfasst von: Sarah Seberg Diemar
Line Tang Møllehave
Nadia Quardon
Louise Lylloff
Betina Heinsbæk Thuesen
Allan Linneberg
Niklas Rye Jørgensen
Publikationsdatum: 01.12.2020
Verlag: Springer London
Erschienen in: Archives of Osteoporosis / Ausgabe 1/2020
Print ISSN: 1862-3522
Elektronische ISSN: 1862-3514
DOI: https://doi.org/10.1007/s11657-020-00715-6

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Springer Medizin

Effects of age and sex on osteocalcin and bone-specific alkaline phosphatase—reference intervals and confounders for two bone formation markers

Abstract

Summary

Objective

Methods

Results

Conclusion

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Springer Medizin

Abstract

Summary

Objective

Methods

Results

Conclusion

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