Background
Diabetic nephropathy (DN) is a major microvascular complication of diabetes mellitus, as well as the leading cause of end-stage renal disease [
1]. A pathological change associated with DN is the accumulation of normal and abnormal extracellular matrix components (ECM) in renal glomeruli and interstitium [
2]. TGFβ is a secreted protein that plays a critical role in renal fibrosis and the accumulation of ECM [
3], and intraperitoneal injections of TGFβ alone were sufficient to initiate a prominent renal fibrotic response [
4]. Further, TGFβ isoforms and their receptors were upregulated in both experimental models of DN, as well as in human DN [
5,
6]. We chose to focus on TGFβ1 because it is the most abundantly expressed isoform in the kidney, and has been most closely linked to the pathophysiology of DN [
6]. Additionally, it was reported that TGFβ1 was stimulated by high glucose levels, and was chiefly expressed in renal tubular epithelial cells in diabetic mice [
7].
TGFβ1 binds to the TGFβR-IΙ, which in turn recruits the binding of TGFβR-I to form a heterotetramer. TGFβR-I subsequently phosphorylates the Smad proteins [
8,
9], after which the activated Smad2/Smad3 associates with Smad4, and the complex translocates to the nucleus where it is involved in regulating transcriptional responses on target genes [
10]. Ultimately, the predominant effect of TGFβ is to promote ECM accumulation.
Astragalus (
Astragalus membranaceus) has long been known in traditional Chinese medicine as an immune-modulating herb. In clinical practice, administration of astragalus has achieved widespread use in the treatment of diabetes, and in the treatment of kidney abnormalities caused by diabetes [
11,
12]. Polysaccharoses, astragaloside, isoflavones, and saponin glycosides are the primary astragalus extracts [
13]. Recent studies have demonstrated that astragalus has an antifibrotic effect in a rat model, and can inhibit the expression of TGFβ1, reduce ECM synthesis, and block tubular epithelial-to-mesenchymal transition (EMT) processes [
14,
15]. Results of a meta-analysis revealed that astragalus injection had therapeutic effects in DN patients, including reduced urine protein and improved renal function [
16]. Astragaloside IV, one of the main active ingredients of astragalus, was shown to ameliorate podocyte apoptosis, prevent acute kidney injury, and attenuate glycated albumin-induced EMT in renal proximal tubular cells [
17,
18].
Hence, understanding the mechanisms underlying the administration of astragalus as a treatment for DN is essential for its application in clinical therapy. To this end, we employed a DN model to investigate the effects of astragalus injection on the TGFβ/Smad pathway.
Methods
Chemicals and reagents
Astragalus injection was purchased from the Chengdu di’aojiuhong pharmaceutical factory, Chengdu, China.
Experimental animals and treatment
All experiments were performed in accordance with the guidelines on Ethical Standards for investigations in animals, and the study was approved by the Beijing University of Chinese Medicine animal research committee. Sixteen male KKAy mice (9–11 weeks of age) weighing 25–28 g were used in the current experiments. Eight male C57BL/6J mice (9–11 weeks of age) weighing 23–25 g were used as age-matched controls. All mice were purchased from the Animal Center of the Chinese Academy of Medical Science (Beijing, China), and were raised in the Clinical Institute of China-Japan Friendship Hospital (Beijing, China). During the conduct of the experimental protocol, the KKAy mice were allowed access to high-fat diet (HFD) and water ad libitum. To serve as a control, the C57BL/6J mice were fed a normal diet and allowed ad libitum access to water.
At 14 weeks of age, blood samples were obtained from the tail vein of the mice for the purpose of measuring blood glucose. A mouse with blood glucose levels above 13.9 mM was considered diabetic. The KKAy mice were randomly divided into the model group (MG, n = 8), and the treatment group (TG, n = 8), with an equivalent distribution of average body weights and blood glucose levels between the two groups. The C57BL/6J mice were used as the control group (CG, n = 8). The treatment group received daily intraperitoneal injections of astragalus (0.03 ml/10 g.d), while the model group received an injection of an equivalent volume of saline. The mice were housed individually in plastic cages with ad libitum access to food and water throughout the experimental periods.
Weekly body weight measurements were conducted and no differences were detected among the groups. Blood samples for the determination of blood glucose levels were taken from the tip of the tail every four weeks using the BREEZE2 Blood Glucose Test Strips (Bayer HealthCare, USA). At 24 weeks of age, all the mice were deprived of food pellets for 10 h, and blood was collected from the orbital plexus. Samples were kept on ice for 1 h, and the plasma was separated by centrifugation at 2000 rpm for 15 min at 4°C. Plasma samples were subsequently stored at -20°C until analysis. A portion of the kidney tissues collected were excised and frozen instantly in liquid nitrogen for the polymerase chain reaction (PCR) and western blotting assays. The remaining tissue was fixed in 4% buffered paraformaldehyde for hematoxylin and eosin (HE) staining and Masson staining.
Biochemical analysis
Mice were euthanized after the collection of blood samples at 24 weeks of age. Blood urea nitrogen (BUN) and plasma creatinine (CREA) levels were measured by an Automated Biochemical Analyzer (Hitachi, Japan).
Urine albumin analysis
At 24 weeks, mice were transferred to metabolic cages for 24 h for the purpose of collecting urine samples. Urine albumin concentrations were determined using an automatic biochemistry analyzer (DADE Xpand, USA). Albuminuria in mice was expressed as milligrams (mg) per 24 h.
Renal histological analysis
Kidney sections were fixed in 4% buffered paraformaldehyde, embedded in paraffin, and cut into 4-μm-thick sections which were prepared for HE and Masson staining.
Analysis of TGFβ1, TGFβR-I, Smad3, and Smad7 mRNA expressions by Reverse transcriptase-PCR
Total RNA was extracted from the kidney samples using Trizol (Invitrogen, CA, USA), and the total RNA concentration and purity were determined by measuring the OD260 and OD280 ratio. RNA was reverse-transcribed using GoScript Reverse Transcription System (Promega, USA), following the manufacturer protocol. Primers for PCR (Table
1) were designed and synthesized by Sangon Biotech Co., Ltd (Shanghai, China). PCR reactions were performed in a thermal cycler (Bio-Rad Laboratory, USA) using the following conditions: 95°C for 5 min followed by 36 cycles of 95°C for 30 s, 50°C for 30 s, 72°C for 40 s, and 72°C for 8 min. The PCR products were analyzed by gel electrophoresis using a 1% agarose gel. The quantity of specific mRNA was normalized as a ratio to the amount of β-actin mRNA.
Table 1
PCR sequences and PCR products
TGF-β1 | 493 bp | TCCCTCAACCTCAAATTATTCA | GCGGTCCACCATTAGCAC |
TGFβ-RI | 172 bp | GGCGAAGGCATTACAGTGTT | TGCACATACAAATGGCCTGT |
Smad7 | 309 bp | ACAGAAAGTGCGGAGCAAGAT | CTGATGAACTGGCGGGTGTAG |
Smad3 | 232 bp | GGGCCAACAAGTCAACAAGT | CTGGCTGGCTAAGGAGTGAC |
β-actin | 243 bp | GAAATCGTGCGTGACATTAAGG | CACGTCACACTTCATGATGGAG |
Western blot analysis for TGFβ1, TGFβR-I, Smad3/7 and p-Smad3
The lysates were clarified by centrifugation and the supernatants collected. Protein concentrations were determined using the bicinchoninic acid assay (BCA) Protein Assay (Applygen, Beijing, China). Equivalent amounts of tissue protein (80 μg) were resolved on SDS polyacrylamide gels, and transferred by electroblotting to polyvinylidene difluoride (PVDF) membranes. The membranes were blocked in 5% (W/V) nonfat milk at room temperature for 1 h, and then incubated overnight at 4°C with the primary antibody against Smad3 (dilution 1: 200, Santa Cruz, CA, USA), Smad7 (dilution 1:200, Santa Cruz, CA, USA), p-Smad3 (dilution 1:1000, Epitomics, CA, USA), and β-actin (dilution 1: 1000, Santa Cruz, CA, USA). After washing in a buffer containing Tris-buffered saline (TBS), with 0.1% Tween, the membranes were incubated with horseradish peroxidase (HRP)-linked anti-mouse secondary antibody at a dilution of 1:3000. Following washing in 0.1% Tween TBS buffer, the immunolabeled proteins were detected by enhanced chemiluminescense reagents (Applygen, Beijing, China). The density of the detected bands was analyzed by Quantity One software.
Statistical analysis
Numerical data were expressed as means ± standard deviation (SD) of at least three independent experiments. Differences in group means were examined using analysis of variance (ANOVA), and resulting P values < 0.05 were considered statistically significant.
Discussion
Diabetic nephropathy, one of the most frequent chronic microvascular complications of diabetes mellitus, is the leading cause of end-stage kidney failure [
19,
20]. The main pathological changes manifest in DN are accumulation of ECM, degeneration of tubular epithelial cells, atrophy, and even the disappearance of some tubules, thickening of basal membranes, and infiltration of inflammatory cells in the mesenchyme [
21,
22]. The KKAy mouse, a well-established model of type-2 diabetes, was produced by transferring the yellow obese gene (Ay allele) into the KK/Ta mouse [
23]. In a previous study, KKAy mice developed obesity, hyperglycemia, and albuminuria by 14-weeks of age. Furthermore, HE staining demonstrated thickening of the glomerular basement membrane, and vacuolar degeneration in the renal tubular epithelial cells. Further, Masson staining of histological sections revealed obvious glomerular sclerosis and interstitial fibrosis in the KKAy mouse, which was consistent with findings in previous studies [
22].
The Chinese herb astragalus is an effective medical prescription used clinically for the treatment of DN [
16,
24]. All of the major constituents of astragalus have been shown to differentially lower high blood glucose levels and improve impaired glucose tolerance in models of type 2 diabetes [
25,
26]. In the current study, injection of astragalus for 10 weeks produced a mild hypoglycemic effect as suggested in our data above. However, glycemic control using insulin was demonstrated to ameliorate DN in STZ-DM rats [
27], which suggested that astragalus administration produces other renoprotective mechanisms beyond sugar lowering effects. Diabetic albuminuria is an early hallmark of DN, and is always associated with the development of characteristic histopathology features. Results including aggravated kidney injuries such as albuminuria, glomerular sclerosis, and interstitial fibrosis in KKAy mice further supported this notion. However, treatment with astragalus attenuated theses changes in diabetic kidneys. The idea that injections of astragalus can improve renal function through inhibiting the EMT process and subsequent collagen production may provide further support of these results [
28]. Moreover, it was found that levels of serum creatinine and blood urea nitrogen were significantly elevated in the KKAy mouse. Furthermore, the treatment group exhibited milder symptoms compared to the model group following the injection of astragalus, which indicated that astragalus administration could ameliorate the deterioration of renal function. Taken together, administration of astragalus may be appropriate for controlling blood glucose levels and body weight. Specifically, astragalus can reverse changes in renal histopathology and attenuate albuminuria, which could lead to improvement in renal function. Thus, there is a need to investigate the molecular mechanisms of astragalus administration for the treatment of DN.
We likewise aimed to investigate the mechanism of astragalus administration as a treatment for DN by focusing on the TGFβ/Smad pathway. TGFβ, a multifunctional cytokine that leads to renal fibrosis, plays a crucial role in the pathogenesis of DN. Interestingly, the administration of TGFβ neutralizing antibodies significantly reduced renal fibrosis [
9]. In our previous studies, we concluded using immunohistochemistry that TGFβ1 protein levels were elevated in diabetic kidneys of the KKAy mice. Furthermore, we also found that TGFβ1 was mainly expressed in the cytoplasm of the renal tubular epithelial cells, and was rarely expressed in glomeruli [
29]. In the current study, the amount of TGFβ1 expression was higher in the model group than in the normal group, which was in accord with previously reported findings [
30,
31]. Additionally, treatment with astragalus significantly reduced TGFβ1 mRNA expression, which suggested that astragalus may play a role in the down-regulation of TGFβ1 at the transcriptional level. Our western blot analyses confirmed that TGFβR-I proteins were up-regulated in the model group; however, increased TGFβR-I expression was not significant at mRNA levels. This phenomenon may be explained by the possibility that the mRNA of TGFβR-I is more prone to degradation, due to its unstable nature.
One of our aims in the current experiment was to investigate the effects of astragalus administration on Smad3 and Smad7. Smad7 is one of the inhibitory Smads, and is known to block the TGFβ signaling pathway by inhibiting Smad2/3 phosphorylation, and thus exerting its anti-fibrotic effect [
32]. Increasing evidence has indicated that disruption of Smad7 may accelerate renal fibrosis [
33,
34]. In our studies, the KKAy mice that exhibited the down-regulation of Smad7 developed more severe renal dysfunction, which provided further confirmation of the effect of Smad7 disruption on renal fibrosis. In addition to the inhibitory Smads, there are receptor-regulated Smads that can transduce the TGFβ signal, such as Smad2 and Smad3. Smad3 is a critical downstream mediator responsible for renal fibrosis that has been shown to function in the diabetes-induced up-regulation of fibronectin and α3 (IV) collagen, and that may play a critical role in the early phase of DN [
35,
36]. It has been well documented that Smad3-deficient mice are protected from renal fibrosis by a reduction in EMT, collagen deposition, and the expression of profibrotic TGFβ target genes [
37,
38]. As indicated by our results, the expression of Smad3 and phosphorylated Smad3 (pSmad3) were increased in the kidneys of diabetic mice. Furthermore, the fact that the administration of astragalus suppressed the expression and phosphorylation of Smad3, and promoted the expression of Smad7, which resulted in improved renal conditions, provides further evidence for the effectiveness of astragalus in the treatment of DN.
Conclusions
In conclusion, we believe that DN is caused by an imbalance in the TGFβ/Smad pathway, and that as a result, fibronectin (FN) increases and assembles, which in turn leads to glomerular sclerosis and interstitial fibrosis. Consistent with our theory, the astragalus injection appeared to alleviate DN by suppressing Smad3 and p-Smad3, as well as the expression of TGFβR-I. Concurrently, astragalus also exerts its effect by reducing the mRNA level of TGFβ1, and promoting Smad7 expression. Collectively, these results demonstrated that astragalus administration could be a potential treatment for DN, and that astragalus could ameliorate the outcomes associated with DN by hindering the TGFβ/Smad pathway.
Competing interests
The authors have declared that there is no conflict of interest.
Authors’ contributions
YN carried out the animal experiments, performed the statistical analysis and drafted the manuscript. YY participated in the western blot assay. WS involved in the extraction of RNA and RT-PCR, XC and DJ participated in the HE staining and discussion of the experiment. SL and QW formulated the original ideas and working hypothesis. QW is the owner of the research grant and revised the draft of manuscript. All authors read and approved the final manuscript.