The online version of this article (doi:10.1186/1476-9255-9-9) contains supplementary material, which is available to authorized users.
Seung-Hyung Kim and Young-Cheol Lee contributed equally to this work.
SH Kim, BK Kim, and YC Lee participated in the design of the study, data analyses, and manuscript preparation. SH Kim, BK Kim, and YC Lee conducted the assays and analyses. All authors read and approved the final manuscript.
Allergic asthma is a chronic inflammatory lung disease that is characterized by airway hyperresponsiveness (AHR) to allergens, airway oedema, increased mucus secretion, excess production of T helper-2 (Th2) cytokines, and eosinophil accumulation in the lungs. Corni fructus (CF) is a fruit of Cornus officinalis Sieb. Et. Zucc. (Cornaceae) and has been used in traditional Korean medicine as an anti-inflammatory, analgesic, and diuretic agent. To investigate the anti-asthmatic effects of CF and their underlying mechanism, we examined the influence of CF on the development of pulmonary eosinophilic inflammation and airway hyperresponsiveness in a mouse model of allergic asthma.
In this study, BALB/c mice were systemically sensitized to ovalbumin (OVA) by intraperitoneal (i.p.), intratracheal (i.t.) injections and intranasal (i.n.) inhalation of OVA. We investigated the effect of CF on airway hyperresponsiveness, pulmonary eosinophilic infiltration, various immune cell phenotypes, Th2 cytokine production, and OVA-specific immunoglobulin E (IgE) production.
The CF-treated groups showed suppressed eosinophil infiltration, allergic airway inflammation, and AHR via reduced production of interleuin (IL) -5, IL-13, and OVA-specific IgE.
Our data suggest that the therapeutic effects of CF in asthma are mediated by reduced production of Th2 cytokines (IL-5), eotaxin, and OVA-specific IgE and reduced eosinophil infiltration.
Authors’ original file for figure 112950_2011_226_MOESM1_ESM.jpeg
Authors’ original file for figure 212950_2011_226_MOESM2_ESM.jpeg
Authors’ original file for figure 312950_2011_226_MOESM3_ESM.jpeg
Authors’ original file for figure 412950_2011_226_MOESM4_ESM.jpeg
Authors’ original file for figure 512950_2011_226_MOESM5_ESM.jpeg
Authors’ original file for figure 612950_2011_226_MOESM6_ESM.jpeg
Authors’ original file for figure 712950_2011_226_MOESM7_ESM.pdf
Authors’ original file for figure 812950_2011_226_MOESM8_ESM.pdf
Authors’ original file for figure 912950_2011_226_MOESM9_ESM.pdf
Seo YB, Kil GJ, Lee YG, Lee YC: Study on the effects of Corni fructus about the anti-allergic action. The Korea Journal of Herbology. 2002, 17 (1): 1-12.
Du WF, Wang MY, Cai BC: Effect of polysaccharides in crude and processed Cornus officinalis on the immunologic function of mice with immunosuppression induced. Zhong Yao Cai. 2008, 31 (5): 715-717. PubMed
Jang IM: Treatise on Asian Herbal Medicines. 2003, Haksulpyunsu-kwan in Research Institute of Natural Products of Seoul National University: Seoul, 477:
Price KR, Johnson IT, Fenwick GR: The chemistry and biological significance of saponins in foods and feeding stuffs. Crit Rev Food Sci Nutr. 1987, 26: 127-135.
Mahato SB, Sarkar SK, Poddar G: Triterpenoid saponins. Phytochemistry. 1988, 27: 3037-3067. 10.1016/0031-9422(88)80001-3. CrossRef
Wang B, Jiang Z: Study of oleanolic acid. Chin Pharm J. 1992, 27: 393-397.
Yamahara J, Mibu H, Sawada T, Fujimura H, Takino S, Yoshikawa M, Kitagawa I: Biologically active principles of crude drugs. Antidiabetic principles of corni fructus in experimental diabetes induced by streptozotocin. Yakugaku Zasshi. 1981, 101: 86-90. PubMed
Qian DS, Zhu YF, Zhu Q: Effect of alcohol extract of Cornus officinalis Sieb. et Zucc. on GLUT4 expression in skeletal muscle in type 2 (non-insulin-dependent) diabetic mellitus rats. China J Chin Mater Med. 2001, 26: 859-862.
Jang SM, Yee ST, Choi J, Choi MS, Do GM, Jeon SM, Yeo J, Kim MJ, Seo KI, Lee MK: Ursolic acid enhances the cellular immune system and pancreatic beta-cell function in streptozotocin-induced diabetic mice fed a high-fat diet. Int Immunopharmacol. 2009, 9: 113-119. 10.1016/j.intimp.2008.10.013. CrossRefPubMed
Gleich GJ, Frigas E, Loegering DA, Wassom DL, Steinmuller D: Cytotoxic properties of the eosinophil major basic protein. J Immunol. 1979, 123: 2925-2927. PubMed
Kitamura M, Nakajima T, Imai T, Harada S, Combadiere C, Tiffany HL, Murphy PM, Yoshie O: Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3. J Biol Chem. 1996, 271: 7725-7730. 10.1074/jbc.271.13.7725. CrossRef
Umland SP, Wan Y, Shortall J, Shah H, Jakway J, Garlisi CG, Tian F, Egan RW, Billah MM: Receptor reserve analysis of the human CCR3 receptor in eosinophils and CCR3-transfected cells. J Leukoc Biol. 2000, 67 (3): 441-447. PubMed
Kyriakis JM, Avruch J: Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation. Physiol Rev. 2001, 81: 807-869. PubMed
Duan W, Chan JH, McKay K, Crosby JR, Choo HH, Leung BP, Karras JG, Wong WS: Inhaled p38alpha mitogen-activated protein kinase antisense oligonucleotide attenuates asthma in mice. Am J Respir Crit Care Med. 2005, 71: 571-578. CrossRef
Chen CC, Wang JK: p38 but not p44/42 mitogen-activated protein kinase is required for nitric oxide synthase induction mediated by lipopolysaccharide in RAW 264.7 macrophages. Mol Pharmacol. 1999, 55 (3): 481-488. PubMed
Cho K, Lee HJ, Lee SY, Woo H, Lee MN, Seok JH, Lee CJ: Oleanolic acid and ursolic acid derived from Cornus officinalis Sieb. et Zucc. suppress epidermal growth factor- and phorbol ester-induced MUC5AC mucin production and gene expression from human airway epithelial cells. Phytother Res. 2011, 25 (5): 760-764. CrossRefPubMed
- Effects of Corni fructus on ovalbumin-induced airway inflammation and airway hyper-responsiveness in a mouse model of allergic asthma
- BioMed Central
Neu im Fachgebiet Innere Medizin
Meistgelesene Bücher aus der Inneren Medizin
e.Med Kampagnen-Visual, Mail Icon II